A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the pho...A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the phosphordiester model compound,BDNPP promoted by the Ce2(III)-L complex was investigated by UV-Vis methods at 25,37℃,and pH 7.20.The result showed that the Ce(Ⅲ) complex could hydrolyze the phosphordiester effectively,with the rate constants as k=626.7 and 821.3(mol/L)-1 min-1,and half lives as 127.65 and 97.41min,reapectively under 25 and 37℃.The investigation on the interaction between the complex and CT DNA indicated that the absorbance of CT DNA was increased and the maximum peak(λmax=260 nm) blue-shifted,while the intensity of fluorescence spectra of EB-DNA was gradually weakened.展开更多
文摘A new complex of Ce2L·6H2O(here L=2,6-bis[bis(carboxylatomethyl)amino]methylg-4-chlorophenolate and abbreviated as Cl-HXTA) was synthezied and characterized by IR and EA.Kinetic study on the hydrolysis of the phosphordiester model compound,BDNPP promoted by the Ce2(III)-L complex was investigated by UV-Vis methods at 25,37℃,and pH 7.20.The result showed that the Ce(Ⅲ) complex could hydrolyze the phosphordiester effectively,with the rate constants as k=626.7 and 821.3(mol/L)-1 min-1,and half lives as 127.65 and 97.41min,reapectively under 25 and 37℃.The investigation on the interaction between the complex and CT DNA indicated that the absorbance of CT DNA was increased and the maximum peak(λmax=260 nm) blue-shifted,while the intensity of fluorescence spectra of EB-DNA was gradually weakened.
