The provirus DNA sequence of porcine endogenous retrovirus (PERV) distributed in the pig genome is the major obstacle that restricts the swine as the organ donors in xenotransplantation, and the copy number of PERV va...The provirus DNA sequence of porcine endogenous retrovirus (PERV) distributed in the pig genome is the major obstacle that restricts the swine as the organ donors in xenotransplantation, and the copy number of PERV varies greatly among different breeds and individuals. In the experiment, 67 healthy, female Chinese Experimental Mini-Pigs (CEMPs) aged at 3—6 months were selected from the Animal Husbandry Station of China Agricultural University, the copy number of PERV and types of envelope protein gene (env) were then investigated by means of PCR analysis and Southern blotting. It is showed that the distribution of types of envelope protein gene in Landrace and CEMPs makes little difference, but the proportion of individuals carrying two types of envelope protein gene (env-A and env-B, which is de- noted as env-AB) is much larger than those which carry only one type of envelope protein gene (env-A or env-B). Meanwhile, two endogenous retrovirus free pigs were found for the first time during our research, and the copy number of others is relatively low, which is about 10 to 20. All the results illuminate the genetic diversity of indigenous pig breeds in China and the potential of CEMPs to serve as organ donors in xenotransplantation.展开更多
Foreland basins on the western edge of Ordos are characterized by typical loess geomorphologic features. Its surface is severely cut, and its underground geological structures are complicated.Making use of the advanta...Foreland basins on the western edge of Ordos are characterized by typical loess geomorphologic features. Its surface is severely cut, and its underground geological structures are complicated.Making use of the advantage of CEMP in exploration in frontland as well as successful multi-year exploration experiences in West China, we first employed the CEMP method to conduct exploration on the western edge of the Ordos basin and its surrounding areas. The practices proved that rich and reliable geological results have been achieved with the CEMP method in complicated areas covered with loess. This can provide important evidence for researching the contact relationship among basins and mountains, structural feature of target layers and predict favourable areas.展开更多
文摘The provirus DNA sequence of porcine endogenous retrovirus (PERV) distributed in the pig genome is the major obstacle that restricts the swine as the organ donors in xenotransplantation, and the copy number of PERV varies greatly among different breeds and individuals. In the experiment, 67 healthy, female Chinese Experimental Mini-Pigs (CEMPs) aged at 3—6 months were selected from the Animal Husbandry Station of China Agricultural University, the copy number of PERV and types of envelope protein gene (env) were then investigated by means of PCR analysis and Southern blotting. It is showed that the distribution of types of envelope protein gene in Landrace and CEMPs makes little difference, but the proportion of individuals carrying two types of envelope protein gene (env-A and env-B, which is de- noted as env-AB) is much larger than those which carry only one type of envelope protein gene (env-A or env-B). Meanwhile, two endogenous retrovirus free pigs were found for the first time during our research, and the copy number of others is relatively low, which is about 10 to 20. All the results illuminate the genetic diversity of indigenous pig breeds in China and the potential of CEMPs to serve as organ donors in xenotransplantation.
文摘Foreland basins on the western edge of Ordos are characterized by typical loess geomorphologic features. Its surface is severely cut, and its underground geological structures are complicated.Making use of the advantage of CEMP in exploration in frontland as well as successful multi-year exploration experiences in West China, we first employed the CEMP method to conduct exploration on the western edge of the Ordos basin and its surrounding areas. The practices proved that rich and reliable geological results have been achieved with the CEMP method in complicated areas covered with loess. This can provide important evidence for researching the contact relationship among basins and mountains, structural feature of target layers and predict favourable areas.
