[ Objective] This study aimed to investigate the effects of 5-aminolevulinic acid （ALA） on the content of total flavonoids and relative expression levels of chalcoue sythase （CHS） and chalcone isomerase （CHI） ge...[ Objective] This study aimed to investigate the effects of 5-aminolevulinic acid （ALA） on the content of total flavonoids and relative expression levels of chalcoue sythase （CHS） and chalcone isomerase （CHI） genes in young apples to determine the appropriate ALA concentration and processing time. [Method] Before thinning, young apples were treated with 0 （ CK）, 100,200,300 and 400 mg/L ALA. At 12 d after ALA treatment, the content of total flavonoids in young apples was determined by ultraviolet spectrophotometry. The relative expression levels of CHS and CHI genes in young apples were determined by qPCR. [ Result ] When ALA concentration was 0 - 300 mg/L, the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples were improved with the increase of ALA concentration. As ALA concentration rose to 400 rag/L, various indicators showed a downward trend. Moreover, the content of total flavouoids and relative expression levels of CHS and CHI genes in young apples treated with different concentrations of ALA were improved significantly, which reached the maximum at 9 d and declined since 12 d. [ Conclusion] Compared with CK, spraying young apples with 300 mg/L ALA at 6 -9 d before thinning was conducive to improving the content of flavanoids in thinned young apples.展开更多
基金Supported by Earmarked Fund for Modern Agro-industry Technology Research System of China(GK661001)
文摘[ Objective] This study aimed to investigate the effects of 5-aminolevulinic acid （ALA） on the content of total flavonoids and relative expression levels of chalcoue sythase （CHS） and chalcone isomerase （CHI） genes in young apples to determine the appropriate ALA concentration and processing time. [Method] Before thinning, young apples were treated with 0 （ CK）, 100,200,300 and 400 mg/L ALA. At 12 d after ALA treatment, the content of total flavonoids in young apples was determined by ultraviolet spectrophotometry. The relative expression levels of CHS and CHI genes in young apples were determined by qPCR. [ Result ] When ALA concentration was 0 - 300 mg/L, the content of total flavonoids and relative expression levels of CHS and CHI genes in young apples were improved with the increase of ALA concentration. As ALA concentration rose to 400 rag/L, various indicators showed a downward trend. Moreover, the content of total flavouoids and relative expression levels of CHS and CHI genes in young apples treated with different concentrations of ALA were improved significantly, which reached the maximum at 9 d and declined since 12 d. [ Conclusion] Compared with CK, spraying young apples with 300 mg/L ALA at 6 -9 d before thinning was conducive to improving the content of flavanoids in thinned young apples.
