Background: Uveal melanoma (UVM) is the most common primary intraocular tumor in adults. However, identification of the effective biomarker for the diagnosis and treatment of UVM remains to be explored. Calcium and in...Background: Uveal melanoma (UVM) is the most common primary intraocular tumor in adults. However, identification of the effective biomarker for the diagnosis and treatment of UVM remains to be explored. Calcium and integrin-binding protein 1 (CIB1) is emerging as an important factor in tumor progression. Purpose: To determine the contribution of CIB1 in the diagnosis of UVM. Method: Immunohistochemical staining is used to detect the CIB1 expression level, while Gene Expression Profiling Interactive Analysis 2 (GEPIA2) and UALCAN online tools were used to analyze patient survival and CIB1 correlation genes in UVM. Integrative analysis using STRING and GeneMANIA predicted the correlated genes with CIB1 in UVM. Results: CIB1 expression level in UVM was significantly enhanced when compared with that in paracancerous tissues. A higher CIB1 expression level resulted in a significantly worse disease-free survival as well as overall survival. Moreover, the survival probability of patients was associated with body weight and gender of the patients with UVM. The correlated genes with CIB1 in UVM, and the similarity of the genes in UVM expression and survival heatmap were verified. Furthermore, Gene ontology enrichment analysis revealed that CIB1 and its correlated genes are significantly enriched in ITGA2B-ITGB3-CIB1 complex, regulation of intracellular protein transport and regulation of ion transport. Conclusions: Our novel findings suggested that CIB1 might be a potential diagnostic predictor for UVM, and might contribute to the potential strategy for UVM treatment by targeting CIB1.展开更多
本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长...本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长cDNA。将其克隆到pMD19-T载体,并进行测序分析。将该基因编码区重组于融合表达质粒pET32a中,构建了重组原核表达载体(pET32a-CIB1)。将其转化到BL21(DE3)pLysS宿主菌中,用IPTG进行诱导表达。结果表明,克隆的CIB1基因cDNA与GenBank上登录的牛、猪、猕猴、人、小鼠、大鼠、黑猩猩等动物该基因序列的同源性达90%以上,编码氨基酸的同源性在93%以上,并且该序列包含有完整的开放阅读框,大小为576 bp。实现了高效特异性融合表达,表达产物的分子质量约为38 ku。本研究结果为进一步研究CIB1蛋白功能打下良好的基础。展开更多
目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,1...目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,176_191 del 16,235del C,299_300 del AT)、GJB3(C538T,G547A)、mt DNA 12S r RNA(A1555G,C1494T)和SLC26A4(IVS7_2A>G,A2168G)致聋基因突变,对照组采用150名健康人,外周静脉采集EDTA抗凝管血液成分,提取基因组DNA,通过PCR扩增含CIB2基因196C>T,272T>C和297 C>G的基因片段,扩增产物直接测序鉴定其基因突变的位点.结果在337名耳聋学生和150名健康人群中均未检测到CIB2基因196C>T,272T>C和297 C>G突变.结论 CIB2基因196C>T,272T>C和297C>G并非是云南省非综合征型耳聋患者携带的基因突变热点,为该地区确定聋病基因筛查谱提供重要依据.展开更多
文摘Background: Uveal melanoma (UVM) is the most common primary intraocular tumor in adults. However, identification of the effective biomarker for the diagnosis and treatment of UVM remains to be explored. Calcium and integrin-binding protein 1 (CIB1) is emerging as an important factor in tumor progression. Purpose: To determine the contribution of CIB1 in the diagnosis of UVM. Method: Immunohistochemical staining is used to detect the CIB1 expression level, while Gene Expression Profiling Interactive Analysis 2 (GEPIA2) and UALCAN online tools were used to analyze patient survival and CIB1 correlation genes in UVM. Integrative analysis using STRING and GeneMANIA predicted the correlated genes with CIB1 in UVM. Results: CIB1 expression level in UVM was significantly enhanced when compared with that in paracancerous tissues. A higher CIB1 expression level resulted in a significantly worse disease-free survival as well as overall survival. Moreover, the survival probability of patients was associated with body weight and gender of the patients with UVM. The correlated genes with CIB1 in UVM, and the similarity of the genes in UVM expression and survival heatmap were verified. Furthermore, Gene ontology enrichment analysis revealed that CIB1 and its correlated genes are significantly enriched in ITGA2B-ITGB3-CIB1 complex, regulation of intracellular protein transport and regulation of ion transport. Conclusions: Our novel findings suggested that CIB1 might be a potential diagnostic predictor for UVM, and might contribute to the potential strategy for UVM treatment by targeting CIB1.
文摘本研究旨在克隆小尾寒羊钙粘和蛋白1(Calcium and integrin binding protein 1,CIB1)基因cDNA全长,并在原核载体中表达。参照已发表的CIB1基因的核苷酸序列,设计了1对特异性引物,采用RT-PCR法,从小尾寒羊睾丸组织中扩增获得CIB1基因全长cDNA。将其克隆到pMD19-T载体,并进行测序分析。将该基因编码区重组于融合表达质粒pET32a中,构建了重组原核表达载体(pET32a-CIB1)。将其转化到BL21(DE3)pLysS宿主菌中,用IPTG进行诱导表达。结果表明,克隆的CIB1基因cDNA与GenBank上登录的牛、猪、猕猴、人、小鼠、大鼠、黑猩猩等动物该基因序列的同源性达90%以上,编码氨基酸的同源性在93%以上,并且该序列包含有完整的开放阅读框,大小为576 bp。实现了高效特异性融合表达,表达产物的分子质量约为38 ku。本研究结果为进一步研究CIB1蛋白功能打下良好的基础。
文摘目的探讨云南省部分地区特教学校聋生携带钙整合素结合蛋白2(calcium-and integrin-binding protein 2,CIB2)基因196C>T,272T>C和297 C>G突变的频率.方法实验组选取337名非综合征型耳聋学生,已明确其全部未携带GJB2(35 del G,176_191 del 16,235del C,299_300 del AT)、GJB3(C538T,G547A)、mt DNA 12S r RNA(A1555G,C1494T)和SLC26A4(IVS7_2A>G,A2168G)致聋基因突变,对照组采用150名健康人,外周静脉采集EDTA抗凝管血液成分,提取基因组DNA,通过PCR扩增含CIB2基因196C>T,272T>C和297 C>G的基因片段,扩增产物直接测序鉴定其基因突变的位点.结果在337名耳聋学生和150名健康人群中均未检测到CIB2基因196C>T,272T>C和297 C>G突变.结论 CIB2基因196C>T,272T>C和297C>G并非是云南省非综合征型耳聋患者携带的基因突变热点,为该地区确定聋病基因筛查谱提供重要依据.
