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DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤
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作者 邓永 唐腾骞 +1 位作者 张庆仪 张雷达 《第三军医大学学报》 CAS CSCD 北大核心 2020年第24期2375-2381,共7页
目的探讨氯离子通道2型(type 2 chloride channel,ClC2)及其阻断剂4,4-二异硫氰基芪-2,2’-二磺酸(4,4’-diisothiocyanostilbene-2,2’-disulfonic acid,DIDS)在肝缺血再灌注损伤中的作用。方法成年SD大鼠以经典法建立肝缺血再灌注模... 目的探讨氯离子通道2型(type 2 chloride channel,ClC2)及其阻断剂4,4-二异硫氰基芪-2,2’-二磺酸(4,4’-diisothiocyanostilbene-2,2’-disulfonic acid,DIDS)在肝缺血再灌注损伤中的作用。方法成年SD大鼠以经典法建立肝缺血再灌注模型。采用q-PCR技术对缺血再灌注后肝组织中ClC2的表达进行检测,采用肝功能血生化分析、活性氧浓度检测、ELISA及流式细胞技术观察缺血再灌注及应用DIDS后对肝功能、氧自由基水平、炎性细胞因子及细胞周期等方面的影响。结果缺血再灌注后肝组织中ClC2 mRNA表达显著升高(P<0.05),并伴随有活性氧浓度升高(P<0.05)、谷丙及谷草转氨酶升高(P<0.05)和炎性细胞因子浓度升高。术后即时使用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度、活性氧水平及炎性因子表达方面均有所逆转(P<0.05),术后8 h应用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度及iNOS(一氧化氮合酶)表达方面有显著差异(P<0.05),而预用DIDS 1 h组相比缺血再灌注组未观察到统计学差异。结论DIDS可以对肝缺血再灌注损伤引起的肝实质损害、炎性反应及氧化应激起保护作用。 展开更多
关键词 肝脏 缺血再灌注损伤 DIDS clc2
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Numerical simulation of stratified turbulent two-phase flow in aquatic environment
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作者 Shen, Yong-Ming Wang, Ya-Ling Liang, Chuan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2000年第2期24-28,共5页
The two fluid model of stratified turbulent two phase flow in aquatic environment is developed in this paper. The motion of each phase is described by a unified multi fluid model in an Eulerian coordinate system. T... The two fluid model of stratified turbulent two phase flow in aquatic environment is developed in this paper. The motion of each phase is described by a unified multi fluid model in an Eulerian coordinate system. The laws of turbulent transportation for each phase, and the restriction of each other between the two phases are completely simulated. The complex two phase turbulence with strong buoyancy effects is selected to examine numerically. The extensive experimental data obtained in stratified flow are used here. Comparison of the results of numerical simulation with the experimental data is conducted. It has shown that the results of numerical simulation are satisfactory. 展开更多
关键词 stratified flow two phase turbulence two fluid model aquatic environment CLC number: X131 2 Document code: A
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几种不太常见的国产雷达
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作者 樊林 《世界航空航天博览(B版)》 2004年第02B期41-43,共3页
CLC—1近程搜索雷达,可以边行进边工作,具有很强的反杂波能力,可迅速捕捉和跟踪超低空来袭目标,并引导火炮实施有效反击。
关键词 CLC一1近程搜索雷达 CLC一2雷达 反杂波能力 工作频段 中国 雷达产品
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Rescuing the Golgi from heat damages by ATG8:restoration rather than clean-up
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作者 Anni Luo Jian-Xiang Liu 《Stress Biology》 2023年第1期219-221,共3页
High temperature stress poses significant adverse effects on crop yield and quality.Yet the molecular mechanisms underlying heat stress tolerance in plants/crops,especially regarding the organellar remodeling and home... High temperature stress poses significant adverse effects on crop yield and quality.Yet the molecular mechanisms underlying heat stress tolerance in plants/crops,especially regarding the organellar remodeling and homeostasis,are largely unknown.In a recent study,Zhou et al.reported that autophagy-related 8(ATG8),a famous regulator involved in autophagy,plays a new role in Golgi restoration upon heat stress.Golgi apparatus is vacuolated following short-term acute heat stress,and ATG8 is translocated to the dilated Golgi membrane and interacts with CLATHRIN LIGHT CHAIN 2(CLC2)to facilitate Golgi restoration,which is dependent on the ATG conjugation system,but not of the upstream autophagic initiators.These exciting findings broaden the fundamental role of ATG8,and elucidate the organelle-level restoration mechanism of Golgi upon heat stress in plants. 展开更多
关键词 ATG8 AUTOPHAGY clc2 ER Golgi restoration Heat stress
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H_2ClCS单分子分解反应机理的理论研究 被引量:3
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作者 刘艳 任宏江 王艳丽 《分子科学学报》 CAS CSCD 北大核心 2011年第6期416-421,共6页
用量子化学B3LYP/6-311+G(d,p)方法优化了H2ClCS单分子分解反应驻点物种的几何构型,并在相同水平上通过频率计算和内禀反应坐标(IRC)分析对过渡态结构及连接性进行了验证.用QCISD(T)/6-311++G(d,p)方法计算各物种的单点能,并对总能量进... 用量子化学B3LYP/6-311+G(d,p)方法优化了H2ClCS单分子分解反应驻点物种的几何构型,并在相同水平上通过频率计算和内禀反应坐标(IRC)分析对过渡态结构及连接性进行了验证.用QCISD(T)/6-311++G(d,p)方法计算各物种的单点能,并对总能量进行了零点能校正.利用经典过渡态理论(TST)与变分过渡态理论(CVT)并结合小曲率隧道效应模型(SCT),计算了主反应通道在200 K~2 000 K温度范围内的速率常数kTST,kCVT和kCVT/SCT.结果表明,H2ClCS存在8条可能的裂解通道,可生成产物P1(HCS+HCl),P2(CSH+HCl),P3(H2S+CCl)和P4(ClHCS+H).所有反应均为吸热反应.相对于H2ClCS,各产物能量分别为60.9,222.9,275.7和156.9 kJ.mol-1.通道H2ClCS→TS3→P1的反应势垒为81.5 kJ.mol-1,是标题反应的主反应通道,其余通道为次反应通道.在200 K~2 000 K温度区间内得到主反应通道的表观反应速率常数三参数表达式为.kCVT/SCT=8.7610T0.79exp(-4 053.6/T)s-1. 展开更多
关键词 H2ClCS 反应机理 分解反应
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Effect of Moxibustion on IL-1β and IL-2 in Rat Models of Rheumatoid Arthritis 被引量:33
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作者 胡玲 Dirckinck-Holmfeld Lars 宋小鸽 唐照亮 蔡荣林 何璐 罗磊 《Journal of Acupuncture and Tuina Science》 2010年第3期149-153,共5页
Objective: To observe the influence on IL-1β and IL-2 in rat models with rheumatoid arthritis after moxibustion on Shenshu (BL 23) and Zusanli (ST 36) points, and to discuss the mechanism of moxibustion. Methods... Objective: To observe the influence on IL-1β and IL-2 in rat models with rheumatoid arthritis after moxibustion on Shenshu (BL 23) and Zusanli (ST 36) points, and to discuss the mechanism of moxibustion. Methods: Fifty male Wistar rats were divided randomly into 5 groups, control group, model group, drug group, moxibustion group, and laser group, 10 for each. Four groups except the normal group were built on the model of rheumatoid arthritis. The changes of body weight and plantar circumference were measured and the level of IL-1β, IL-2 in sera were examined by ELISA. Results: Compared with the model group, the weight and plantar circumference of rats in the moxibustion group were improved significantly after treatment (P〈0.01), and the improvement of plantar circumference also had significant differences compared with the drug group and the laser group (P〈0.05). The level of IL-1β, IL-2 in sera were down regulated in the moxibustion group and the laser group, which had statistical differences compared with the model group (P〈0.05), but no statistical differences were found when comparing with the drug group. Conclusion: Moxibustion obviously improves the toe tumefaction of the rats with rheumatoid arthritis, which is better than CO2 laser of 10.6μm. On the aspect of decreasing the amount of IL-1β, IL-2, CO2 laser of 10.6μm is similar with moxibustion. 展开更多
关键词 Moxibustion Therapy Arthritis Rheumatoid Interleukin-1 beta INTERLEUKIN-2 Rats [CLC Number] R2-03
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