With the complete success of the 2nd stage of Chinese Manned Space Program(CMSP),several science researches have been performed on Tiangong-1 experimental spacelab, which was docked with three Shenzhou spaceships one ...With the complete success of the 2nd stage of Chinese Manned Space Program(CMSP),several science researches have been performed on Tiangong-1 experimental spacelab, which was docked with three Shenzhou spaceships one after another. The China's real spacelab, Tiangong-2 will be launched in 2015, docked with a Shenzhou spaceship soon. After six months, it will be docked with the first Chinese cargo ship(Tianzhou-1). More space science researches, involving with space biology, fluid physics, fundamental physics, materials science, Earth science, astronomy and space environmental science, will be operated on Tiangong-2 spacelab, and crewed and cargo spaceships. Furthermore, the considerable large-scale space utilization of Shina's Space Station is planned. The research fields include yet not limited to space medicine and physiology, space life science and biotechnology, fluid physics and combustion in microgravity, space material science,and fundamental physics in microgravity, space astronomy, Earth science, space physics and space environment utilization, technology demonstration.展开更多
A recombinant transposon Tn5-GmSp, produced by replacing the central BglⅡ fragmentwith a BamHⅠ one of R1033, was utilized to detect Tn5 transposition inhibition in Rhizo-bium leguminosarum. Two types of suicide plas...A recombinant transposon Tn5-GmSp, produced by replacing the central BglⅡ fragmentwith a BamHⅠ one of R1033, was utilized to detect Tn5 transposition inhibition in Rhizo-bium leguminosarum. Two types of suicide plasmids (pXS102 and pCW3/pCW30) were con-structed for Tn5-GmSp transposition. The frequency of transposition in both constructs wasgreatly reduced in the Rhizobium strains where a resident Tn5 was already present. WhenpXS102 was transferred into a Tn5-containing Rhizobium strain, the transconjugants retainedthe deleted forms of pXS102, indicating that the failure of transposition was due to the lossof the unstable prophage Mu element. In the case of pCW3/pCW30, vectors were not ob-served in the transconjugants although most of them retained the antibiotic resistant markers.This result suggested the integration of Tn5-GmSp into the rhizobial host genome throughrecombination.展开更多
文摘With the complete success of the 2nd stage of Chinese Manned Space Program(CMSP),several science researches have been performed on Tiangong-1 experimental spacelab, which was docked with three Shenzhou spaceships one after another. The China's real spacelab, Tiangong-2 will be launched in 2015, docked with a Shenzhou spaceship soon. After six months, it will be docked with the first Chinese cargo ship(Tianzhou-1). More space science researches, involving with space biology, fluid physics, fundamental physics, materials science, Earth science, astronomy and space environmental science, will be operated on Tiangong-2 spacelab, and crewed and cargo spaceships. Furthermore, the considerable large-scale space utilization of Shina's Space Station is planned. The research fields include yet not limited to space medicine and physiology, space life science and biotechnology, fluid physics and combustion in microgravity, space material science,and fundamental physics in microgravity, space astronomy, Earth science, space physics and space environment utilization, technology demonstration.
基金Project supported by the National Postdoctoral Science Foundation of China.
文摘A recombinant transposon Tn5-GmSp, produced by replacing the central BglⅡ fragmentwith a BamHⅠ one of R1033, was utilized to detect Tn5 transposition inhibition in Rhizo-bium leguminosarum. Two types of suicide plasmids (pXS102 and pCW3/pCW30) were con-structed for Tn5-GmSp transposition. The frequency of transposition in both constructs wasgreatly reduced in the Rhizobium strains where a resident Tn5 was already present. WhenpXS102 was transferred into a Tn5-containing Rhizobium strain, the transconjugants retainedthe deleted forms of pXS102, indicating that the failure of transposition was due to the lossof the unstable prophage Mu element. In the case of pCW3/pCW30, vectors were not ob-served in the transconjugants although most of them retained the antibiotic resistant markers.This result suggested the integration of Tn5-GmSp into the rhizobial host genome throughrecombination.
