目的探索即刻早期抗原(immediate early antigen,IE抗原)检测在骨髓移植术后人巨细胞病毒感染中的诊断价值。方法回顾性分析骨髓移植术后160份外周血标本,根据外周血巨细胞病毒被膜抗原pp65的不同分为感染组和对照组,运用免疫组化法检...目的探索即刻早期抗原(immediate early antigen,IE抗原)检测在骨髓移植术后人巨细胞病毒感染中的诊断价值。方法回顾性分析骨髓移植术后160份外周血标本,根据外周血巨细胞病毒被膜抗原pp65的不同分为感染组和对照组,运用免疫组化法检测外周血人巨细胞病毒IE抗原和被膜抗原pp65。结果20名患者中,实验组16名(136份血标本),对照组4名(24份血标本)。160份外周血标本中,人巨细胞病毒pp65抗原阳性标本95份(59.38%),IE抗原阳性94份(56.75%)(P=0.91);IE抗原和pp65抗原术后检出时间分别为2.44±1.38周和3.17±1.16周(P=0.031)。结论IE抗原检测能发现早期特异性反应HCMV感染。展开更多
Many viral epitope specific T cell receptors （TCRs） in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 （CDR3）. However, it is ...Many viral epitope specific T cell receptors （TCRs） in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 （CDR3）. However, it is not sure whether the conserved motifs can also be found in TCR β chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus （CMV） pp65 peptide-specific CD8^＋ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8β T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR βchain BV3, BV14, BV21, BV23, BVll families in donor CD8^＋ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence （TX） G （X） A in CDR3 region of TCR α chain and L （XT） G （X） A in TCR β chain.展开更多
文摘目的探索即刻早期抗原(immediate early antigen,IE抗原)检测在骨髓移植术后人巨细胞病毒感染中的诊断价值。方法回顾性分析骨髓移植术后160份外周血标本,根据外周血巨细胞病毒被膜抗原pp65的不同分为感染组和对照组,运用免疫组化法检测外周血人巨细胞病毒IE抗原和被膜抗原pp65。结果20名患者中,实验组16名(136份血标本),对照组4名(24份血标本)。160份外周血标本中,人巨细胞病毒pp65抗原阳性标本95份(59.38%),IE抗原阳性94份(56.75%)(P=0.91);IE抗原和pp65抗原术后检出时间分别为2.44±1.38周和3.17±1.16周(P=0.031)。结论IE抗原检测能发现早期特异性反应HCMV感染。
基金supported by National Natural Science Foundation of China (30771952, 30771971)Program for New Century Excellent Talents in University (NCET-07-0410)+1 种基金Natural Science Foundation of Guangdong Province (07117783)the Major State Basic Research Development Program (973) (2007CB512405)
文摘Many viral epitope specific T cell receptors （TCRs） in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 （CDR3）. However, it is not sure whether the conserved motifs can also be found in TCR β chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus （CMV） pp65 peptide-specific CD8^＋ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8β T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR βchain BV3, BV14, BV21, BV23, BVll families in donor CD8^＋ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence （TX） G （X） A in CDR3 region of TCR α chain and L （XT） G （X） A in TCR β chain.
