Objective:To explore the value of using the venous-arterial carbon dioxide partial pressure difference and the arterial-venous oxygen content difference ratio(ΔP_(CO2)/Ca-v_(O2))as targets to guide early tissue hypop...Objective:To explore the value of using the venous-arterial carbon dioxide partial pressure difference and the arterial-venous oxygen content difference ratio(ΔP_(CO2)/Ca-v_(O2))as targets to guide early tissue hypoperfusion in sepsis in plateau areas.Methods:90 sepsis patients admitted to the Third People’s Hospital of Xining and Golmud People’s Hospital from June 2017 to December 2022 were selected as the research subjects,and they were divided into the Scv_(O2)(central venous oxygen saturation)group and theΔP_(CO2)/Ca-v_(O2)group,with 45 cases in each group.The two groups were treated with early shock resuscitation according to different protocols.The hemodynamic characteristics of the two groups of patients before and after resuscitation were observed,and the volume responsiveness was evaluated.The ROC(receiver operating characteristic)curve was used to analyze the significance ofΔP_(CO2)/Ca-v_(O2),Scv_(O2),lactate,lactate clearance,and urine output in evaluating patient prognosis and the correlation betweenΔP_(CO2)/Ca-v_(O2)and the above indicators was explored.Results:Compared with before resuscitation,after fluid resuscitation,the heart rate(HR),mean arterial pressure(MAP),central venous pressure(CVP),cardiac index(CI),lactate,lactate clearance rate,and urine output of the two groups of patients were significantly improved(P<0.05);in terms of therapeutic effect,the 28-day mortality rate,6-hour fluid balance,and lactic acid clearance of theΔP_(CO2)/Ca-v_(O2)group were better than the Scv_(O2)group.The ROC characteristic curve showed that theΔP_(CO2)/Ca-v_(O2)value can effectively predict the prognosis of patients(AUC=0.907,sensitivity was 97%,specificity was 72.4%,and critical value was 1.84).ΔP_(CO2)/Ca-v_(O2)significantly correlated with Scv_(O2),lactic acid,and lactic acid clearance rate.Conclusion:TheΔP_(CO2)/Ca-v_(O2)value can be used to guide fluid resuscitation in early hypoperfusion in sepsis in plateau areas,improve patients’hemodynamics,reduce lactate indicators,and increase urine output.ΔP_(CO2)/Ca-v_(O2)level>1.84 can effectively improve patient prognosis.展开更多
能量在果蔬保鲜及衰老过程中起着至关重要的作用,为研究O2/CO2气调对西兰花能量代谢及保鲜效果的影响,本实验以自然空气为对照(CK),研究体积分数20%O2+80%CO2、50%O2+50%CO2、80%O2+20%CO2气调环境对西兰花10℃贮藏过程中pH值和丙酮酸...能量在果蔬保鲜及衰老过程中起着至关重要的作用,为研究O2/CO2气调对西兰花能量代谢及保鲜效果的影响,本实验以自然空气为对照(CK),研究体积分数20%O2+80%CO2、50%O2+50%CO2、80%O2+20%CO2气调环境对西兰花10℃贮藏过程中pH值和丙酮酸脱氢酶(pyruvate dehydrogenase,PDH)、琥珀酸脱氢酶(succinate dehydrogenase,SDH)和细胞色素c氧化酶(cytochrome c oxidase,CCO)活力及能荷水平、色调(H值)、VC含量和水分质量分数的影响。结果表明:与50%O2+50%CO2处理组相比,过高比例的CO2(20%O2+80%CO2)处理可引起西兰花pH值的明显降低,进而造成PDH、SDH和CCO活力的下降和能荷水平的降低,最终造成贮藏期间西兰花VC含量、色调和水分质量分数的下降;过高比例的O2(80%O2+20%CO2)处理虽在贮藏前期提高了PDH、SDH和CCO活力,但在后期迅速下降,不利于建立平稳而持续的能量供应体系。适宜的O2/CO2气调如50%O2+50%CO2则可调节西兰花体内pH值和呼吸关键酶活力,建立持续稳定的能量供应体系并很好地维持西兰花贮藏品质。相关性分析表明,能荷水平与西兰花VC含量、色调和水分质量分数具有很高的正相关性,相关系数最高达0.996,因此,能荷变化可作为判断西兰花贮藏条件优劣的衡量指标。展开更多
基金2017 Xining Citizens’Biotechnology Plan Project(Project number:2017-K-15)。
文摘Objective:To explore the value of using the venous-arterial carbon dioxide partial pressure difference and the arterial-venous oxygen content difference ratio(ΔP_(CO2)/Ca-v_(O2))as targets to guide early tissue hypoperfusion in sepsis in plateau areas.Methods:90 sepsis patients admitted to the Third People’s Hospital of Xining and Golmud People’s Hospital from June 2017 to December 2022 were selected as the research subjects,and they were divided into the Scv_(O2)(central venous oxygen saturation)group and theΔP_(CO2)/Ca-v_(O2)group,with 45 cases in each group.The two groups were treated with early shock resuscitation according to different protocols.The hemodynamic characteristics of the two groups of patients before and after resuscitation were observed,and the volume responsiveness was evaluated.The ROC(receiver operating characteristic)curve was used to analyze the significance ofΔP_(CO2)/Ca-v_(O2),Scv_(O2),lactate,lactate clearance,and urine output in evaluating patient prognosis and the correlation betweenΔP_(CO2)/Ca-v_(O2)and the above indicators was explored.Results:Compared with before resuscitation,after fluid resuscitation,the heart rate(HR),mean arterial pressure(MAP),central venous pressure(CVP),cardiac index(CI),lactate,lactate clearance rate,and urine output of the two groups of patients were significantly improved(P<0.05);in terms of therapeutic effect,the 28-day mortality rate,6-hour fluid balance,and lactic acid clearance of theΔP_(CO2)/Ca-v_(O2)group were better than the Scv_(O2)group.The ROC characteristic curve showed that theΔP_(CO2)/Ca-v_(O2)value can effectively predict the prognosis of patients(AUC=0.907,sensitivity was 97%,specificity was 72.4%,and critical value was 1.84).ΔP_(CO2)/Ca-v_(O2)significantly correlated with Scv_(O2),lactic acid,and lactic acid clearance rate.Conclusion:TheΔP_(CO2)/Ca-v_(O2)value can be used to guide fluid resuscitation in early hypoperfusion in sepsis in plateau areas,improve patients’hemodynamics,reduce lactate indicators,and increase urine output.ΔP_(CO2)/Ca-v_(O2)level>1.84 can effectively improve patient prognosis.
文摘能量在果蔬保鲜及衰老过程中起着至关重要的作用,为研究O2/CO2气调对西兰花能量代谢及保鲜效果的影响,本实验以自然空气为对照(CK),研究体积分数20%O2+80%CO2、50%O2+50%CO2、80%O2+20%CO2气调环境对西兰花10℃贮藏过程中pH值和丙酮酸脱氢酶(pyruvate dehydrogenase,PDH)、琥珀酸脱氢酶(succinate dehydrogenase,SDH)和细胞色素c氧化酶(cytochrome c oxidase,CCO)活力及能荷水平、色调(H值)、VC含量和水分质量分数的影响。结果表明:与50%O2+50%CO2处理组相比,过高比例的CO2(20%O2+80%CO2)处理可引起西兰花pH值的明显降低,进而造成PDH、SDH和CCO活力的下降和能荷水平的降低,最终造成贮藏期间西兰花VC含量、色调和水分质量分数的下降;过高比例的O2(80%O2+20%CO2)处理虽在贮藏前期提高了PDH、SDH和CCO活力,但在后期迅速下降,不利于建立平稳而持续的能量供应体系。适宜的O2/CO2气调如50%O2+50%CO2则可调节西兰花体内pH值和呼吸关键酶活力,建立持续稳定的能量供应体系并很好地维持西兰花贮藏品质。相关性分析表明,能荷水平与西兰花VC含量、色调和水分质量分数具有很高的正相关性,相关系数最高达0.996,因此,能荷变化可作为判断西兰花贮藏条件优劣的衡量指标。