Objectives:Thyroid cancer(THCA)is the most common malignant tumor in endocrine system and the incidence has been increasing worldwide.And the number of patients dying from THCA has also gradually risen because the inc...Objectives:Thyroid cancer(THCA)is the most common malignant tumor in endocrine system and the incidence has been increasing worldwide.And the number of patients dying from THCA has also gradually risen because the incidence continues to increase,so the mechanisms related to effective targets is necessary to improve the survival.This study was to preliminarily investigate the effects of the COL4A2 gene on the regulation of thyroid cancer(THCA)cell proliferation and the associated pathways.Methods:Bioinformatics analysis revealed that COL4A2 was closely associated with cancer development.COL4A2 expression in THCA tissues was analyzed using immunohistochemistry,and survival information was determined via Kaplan-Meier curves.The expression of COL4A2 and AKT pathway-related genes were analyzed using qPCR and western blot analyses.Colony formation as well as CCK-8 assays exhibited the cell proliferation level and cell activity,respectively.Downstream of COL4A2 was identified by Gene set enrichment analysis(GSEA).The effects of the COL4A2 and AKT pathways on THCA tumor growth in vivo were determined using a mouse model.Results:Bioinformatics analysis exhibited that COL4A2 plays a significant role in cancer and that the AKT pathway is downstream of COL4A2.THCA patients with high COL4A2 expression had shorter recurrence-free survival.Upregulation of COL4A2 gene expression in 2 THCA cell lines promoted tumor cell growth and activity.The use of AKT pathway blockers also restrained the growth and activity of the 2 THCA cell lines.The use of AKT pathway blockers reduced tumor volume and mass and prolonged mouse survival.Conclusions:COL4A2 can promote the growth as well as development of THCA through the AKT pathway and COL4A2 could be used as a target for THCA.展开更多
目的探究COL4A2基因多态性位点rs9521733与新疆地区汉族腔隙性脑梗死的关联性。方法以2016年3月至2017年9月在新疆医科大学第一附属医院及新疆兵团第七师医院神经内科就诊的汉族406例急性腔隙性脑梗死患者和同期425名对照者为研究对象...目的探究COL4A2基因多态性位点rs9521733与新疆地区汉族腔隙性脑梗死的关联性。方法以2016年3月至2017年9月在新疆医科大学第一附属医院及新疆兵团第七师医院神经内科就诊的汉族406例急性腔隙性脑梗死患者和同期425名对照者为研究对象，收集其临床资料，对受试者的血样提取基因组DNA，运用多重高温连接酶检测反应技术进行COL4A2基因多态性位点rs9521733检测，采用SPSS 17.0软件进行统计学分析。结果COL4A2基因多态性位点rs9521733基因型及等位基因频率在病例组与对照组差异无统计学意义；分层分析后发现，在年龄≥60岁组COL4A2基因多态性位点rs9521733基因型CC明显增加腔隙性脑梗死的发病风险（调整OR＝1.950，95% CI 1.222～3.112，P＝0.005）。结论COL4A2基因多态性位点rs9521733可能与年龄≥60岁腔隙性脑梗死相关。展开更多
文摘Objectives:Thyroid cancer(THCA)is the most common malignant tumor in endocrine system and the incidence has been increasing worldwide.And the number of patients dying from THCA has also gradually risen because the incidence continues to increase,so the mechanisms related to effective targets is necessary to improve the survival.This study was to preliminarily investigate the effects of the COL4A2 gene on the regulation of thyroid cancer(THCA)cell proliferation and the associated pathways.Methods:Bioinformatics analysis revealed that COL4A2 was closely associated with cancer development.COL4A2 expression in THCA tissues was analyzed using immunohistochemistry,and survival information was determined via Kaplan-Meier curves.The expression of COL4A2 and AKT pathway-related genes were analyzed using qPCR and western blot analyses.Colony formation as well as CCK-8 assays exhibited the cell proliferation level and cell activity,respectively.Downstream of COL4A2 was identified by Gene set enrichment analysis(GSEA).The effects of the COL4A2 and AKT pathways on THCA tumor growth in vivo were determined using a mouse model.Results:Bioinformatics analysis exhibited that COL4A2 plays a significant role in cancer and that the AKT pathway is downstream of COL4A2.THCA patients with high COL4A2 expression had shorter recurrence-free survival.Upregulation of COL4A2 gene expression in 2 THCA cell lines promoted tumor cell growth and activity.The use of AKT pathway blockers also restrained the growth and activity of the 2 THCA cell lines.The use of AKT pathway blockers reduced tumor volume and mass and prolonged mouse survival.Conclusions:COL4A2 can promote the growth as well as development of THCA through the AKT pathway and COL4A2 could be used as a target for THCA.
文摘目的探究COL4A2基因多态性位点rs9521733与新疆地区汉族腔隙性脑梗死的关联性。方法以2016年3月至2017年9月在新疆医科大学第一附属医院及新疆兵团第七师医院神经内科就诊的汉族406例急性腔隙性脑梗死患者和同期425名对照者为研究对象，收集其临床资料，对受试者的血样提取基因组DNA，运用多重高温连接酶检测反应技术进行COL4A2基因多态性位点rs9521733检测，采用SPSS 17.0软件进行统计学分析。结果COL4A2基因多态性位点rs9521733基因型及等位基因频率在病例组与对照组差异无统计学意义；分层分析后发现，在年龄≥60岁组COL4A2基因多态性位点rs9521733基因型CC明显增加腔隙性脑梗死的发病风险（调整OR＝1.950，95% CI 1.222～3.112，P＝0.005）。结论COL4A2基因多态性位点rs9521733可能与年龄≥60岁腔隙性脑梗死相关。
