With the advance of wireless communication technologies, small-size and high-performance computing and communication devices are increasingly used in daily life. After the success of second generation mobile system, m...With the advance of wireless communication technologies, small-size and high-performance computing and communication devices are increasingly used in daily life. After the success of second generation mobile system, more interest was started in wireless communications. A Mobile Ad hoc Network (MANET) is a wireless network without any fixed infrastructure or centralized control;it contains mobile nodes that are connected dynamically in an arbitrary manner. The Mobile Ad hoc Networks are essentially suitable when infrastructure is not present or difficult or costly to setup or when network setup is to be done quickly within a short period, they are very attractive for tactical communication in the military and rescue missions. They are also expected to play an important role in the civilian for as convention centers, conferences, and elec-tronic classrooms. The clustering is an important research area in mobile ad hoc networks because it im-proves the performance of flexibility and scalability when network size is huge with high mobility. All mo-bile nodes operate on battery power;hence, the power consumption becomes an important issue in Mobile Ad hoc Network. In this article we proposed an Energy Aware Clustered-Based Multipath Routing (EACMR), which forms several clusters, finds energy aware node-disjoint multiple routes from a source to destination and increases the network life time by using optimal routes.展开更多
寻找栗疫菌的凋亡抑制蛋白(inhibitor of apoptosis proteins,IAPs)类蛋白,明确该蛋白与其他物种IAPs的进化关系,并对CpIAP基因功能进行初步研究。以IAPs家族的典型结构域"BIR"为靶标,对栗疫菌基因组数据库进行BLAST搜索。运...寻找栗疫菌的凋亡抑制蛋白(inhibitor of apoptosis proteins,IAPs)类蛋白,明确该蛋白与其他物种IAPs的进化关系,并对CpIAP基因功能进行初步研究。以IAPs家族的典型结构域"BIR"为靶标,对栗疫菌基因组数据库进行BLAST搜索。运用生物信息学对预测的CpIAP蛋白结构进行分析并构建系统进化树;采用同源重组的策略,构建CpIAP基因缺失突变体,并重新导入该基因全长片段获得互补株。结果从栗疫菌中鉴定了1个IAP基因,Southern blot验证为单拷贝。生物信息学分析表明:CpIAP基因全长2 997 bp,编码920个氨基酸,具有2个BIR结构域;进化关系上CpIAP与人Survivin和酵母IAP较近。CpIAP敲除突变体命名为ΔIAP,ΔIAP菌落形态改变,生长速率减慢,致病力下降,丧失产孢能力。上述结果表明:CpIAP参与了栗疫菌的菌落形态建成、分生孢子形成和致病过程。展开更多
文摘With the advance of wireless communication technologies, small-size and high-performance computing and communication devices are increasingly used in daily life. After the success of second generation mobile system, more interest was started in wireless communications. A Mobile Ad hoc Network (MANET) is a wireless network without any fixed infrastructure or centralized control;it contains mobile nodes that are connected dynamically in an arbitrary manner. The Mobile Ad hoc Networks are essentially suitable when infrastructure is not present or difficult or costly to setup or when network setup is to be done quickly within a short period, they are very attractive for tactical communication in the military and rescue missions. They are also expected to play an important role in the civilian for as convention centers, conferences, and elec-tronic classrooms. The clustering is an important research area in mobile ad hoc networks because it im-proves the performance of flexibility and scalability when network size is huge with high mobility. All mo-bile nodes operate on battery power;hence, the power consumption becomes an important issue in Mobile Ad hoc Network. In this article we proposed an Energy Aware Clustered-Based Multipath Routing (EACMR), which forms several clusters, finds energy aware node-disjoint multiple routes from a source to destination and increases the network life time by using optimal routes.