The COP9 signalosome subunit 6(COPS6)is abnormally overexpressed in many malignancies,yet its precise role in carcinogenesis is unknown.To gain a better understanding of COPS6's role,the authors conducted a pan-ca...The COP9 signalosome subunit 6(COPS6)is abnormally overexpressed in many malignancies,yet its precise role in carcinogenesis is unknown.To gain a better understanding of COPS6's role,the authors conducted a pan-cancer analysis using various bioinformatics techniques such as differential expression patterns,prognostic value,gene mutations,immune infiltration,correlation analysis,and functional enrichment assessment.Results showed that COPS6 was highly correlated with prognosis,immune cell infiltration level,tumor mutation burden,and microsatellite instability in patients with a range of tumor types.This suggests that COPS6 may be a potential target for cancer treatment.Overall,this research provides insight into COPS6's role in cancer development and its potential therapeutic applications.展开更多
BACKGROUND The COP9 signalosome subunit 6(COPS6)has been implicated in cancer progression,while its precise role in most types of cancer remains elusive.AIM To investigate the functional and clinical relevance of COPS...BACKGROUND The COP9 signalosome subunit 6(COPS6)has been implicated in cancer progression,while its precise role in most types of cancer remains elusive.AIM To investigate the functional and clinical relevance of COPS6 across various tumor types using publicly available databases.METHODS We used R software and online analysis databases to analyze the differential expression,prognosis,mutation and related functions of COPS6 in pan-cancer.RESULTS Differential expression analysis and survival analysis demonstrated that COPS6 was highly expressed and associated with high-risk profiles in the majority of cancer types.Possible associations between COPS6 expression level and prognostic outcomes were found using data from public databases.Mutational analysis revealed that missense mutations were the predominant type of COPS6 mutation.Additionally,positive correlations were identified between COPS6 expression level and tumor mutational burden and microsatellite instability in most types of cancer.Immune infiltration analysis demonstrated a negative correlation between COPS6 expression level and CD8+T cell infiltration in certain types of cancer.The correlation between COPS6 expression level and cancerassociated fibroblast infiltration exhibited heterogeneity,in which a positive correlation was found in head and neck squamous cell carcinoma and tenosynovial giant cell tumor,and a negative correlation was identified in diffuse large B-cell lymphoma and thymoma.The correlation between COPS6 expression level and macrophage infiltration was closely related to macrophage type.Gene co-expression and enrichment analysis highlighted transcription elongation factor B polypeptide 2 and G protein pathway suppressor 1 were significantly and positively associated with COPS6 expression level.These genes were predominantly involved in processes,such as ubiquitin-mediated proteolysis and human immunodeficiency virus 1 infection.CONCLUSION In conclusion,this study systematically explored the significance of COPS6 across different tumor types,providing a solid foundation for considering COPS6 as a novel biomarker in cancer research.展开更多
COPS(Concurrent Official Production System)是将产生式系统程序设计语言OPS5和面向进程的程序设计范例相结合,而设计的用于支持协同式问题求解的程序系统。本文详细阐述了COPS通讯原语的设计及其对应的语法和语义。COPS不仅能在单机...COPS(Concurrent Official Production System)是将产生式系统程序设计语言OPS5和面向进程的程序设计范例相结合,而设计的用于支持协同式问题求解的程序系统。本文详细阐述了COPS通讯原语的设计及其对应的语法和语义。COPS不仅能在单机上实现,而且也可以直接的移植到分布式系统上。展开更多
背景与目的:卵巢癌是妇科恶性肿瘤中发病率排名第3、致死率排名第1的疾病。卵巢癌的预后很差,这是由于大部分患者确诊时已处于晚期并且对铂类药物化疗易耐药,异常的DNA修复是导致铂类耐药的重要原因,针对性地干扰DNA损伤修复相关分子可...背景与目的:卵巢癌是妇科恶性肿瘤中发病率排名第3、致死率排名第1的疾病。卵巢癌的预后很差,这是由于大部分患者确诊时已处于晚期并且对铂类药物化疗易耐药,异常的DNA修复是导致铂类耐药的重要原因,针对性地干扰DNA损伤修复相关分子可能是提高铂类药物化疗敏感性的新手段。Ku86是参与非同源末端连接(non-homologous end joining,NHEJ)过程的一个关键分子,能够有效地修复DNA双链断裂(DNA double-strand break,DSB)。方法:使用免疫组织化学染色和细胞免疫荧光法检测Ku86在卵巢癌组织和细胞中的定位。使用RNAi技术下调Ku86,用顺铂处理后使用流式细胞术检测凋亡,使用细胞计数试剂盒(cell counting kit-8,CCK-8)法检测IC_(50)。使用蛋白质印迹法(Western blot)分别检测并分析Ku86与拓扑异构酶Ⅰ(topoisomeraseⅠ,TOP1)及COP9信号复合体的第5种成分(the fifth component of the COP9 signalosome,COPS5)的关系。结果:下调Ku86可以减少由于顺铂引起的细胞凋亡,降低卵巢癌细胞对于顺铂的敏感性。Ku86对顺铂在卵巢癌药物敏感性中的这种影响可能是通过调节TOP1和COPS5发挥作用的。结论:TOP1和COPS5都是与DNA损伤修复有关并能影响铂类药物化疗敏感性的重要分子,下调Ku86会使TOP1的表达增高和COPS5的表达减少。卵巢癌中与铂类药物敏感性有关的生物标志物之间有相关性,靶向Ku86的治疗可能是提高卵巢癌药物敏感性的有效策略。展开更多
文摘The COP9 signalosome subunit 6(COPS6)is abnormally overexpressed in many malignancies,yet its precise role in carcinogenesis is unknown.To gain a better understanding of COPS6's role,the authors conducted a pan-cancer analysis using various bioinformatics techniques such as differential expression patterns,prognostic value,gene mutations,immune infiltration,correlation analysis,and functional enrichment assessment.Results showed that COPS6 was highly correlated with prognosis,immune cell infiltration level,tumor mutation burden,and microsatellite instability in patients with a range of tumor types.This suggests that COPS6 may be a potential target for cancer treatment.Overall,this research provides insight into COPS6's role in cancer development and its potential therapeutic applications.
基金Supported by National Natural Science Foundation of China,No.31900558the Hubei Provincial Youth Talents Program for Public Health,No.WSJKRC2022013Wuhan Young and Middle-Aged Medical Backbone Talents Training Project,No.WHQG201904.
文摘BACKGROUND The COP9 signalosome subunit 6(COPS6)has been implicated in cancer progression,while its precise role in most types of cancer remains elusive.AIM To investigate the functional and clinical relevance of COPS6 across various tumor types using publicly available databases.METHODS We used R software and online analysis databases to analyze the differential expression,prognosis,mutation and related functions of COPS6 in pan-cancer.RESULTS Differential expression analysis and survival analysis demonstrated that COPS6 was highly expressed and associated with high-risk profiles in the majority of cancer types.Possible associations between COPS6 expression level and prognostic outcomes were found using data from public databases.Mutational analysis revealed that missense mutations were the predominant type of COPS6 mutation.Additionally,positive correlations were identified between COPS6 expression level and tumor mutational burden and microsatellite instability in most types of cancer.Immune infiltration analysis demonstrated a negative correlation between COPS6 expression level and CD8+T cell infiltration in certain types of cancer.The correlation between COPS6 expression level and cancerassociated fibroblast infiltration exhibited heterogeneity,in which a positive correlation was found in head and neck squamous cell carcinoma and tenosynovial giant cell tumor,and a negative correlation was identified in diffuse large B-cell lymphoma and thymoma.The correlation between COPS6 expression level and macrophage infiltration was closely related to macrophage type.Gene co-expression and enrichment analysis highlighted transcription elongation factor B polypeptide 2 and G protein pathway suppressor 1 were significantly and positively associated with COPS6 expression level.These genes were predominantly involved in processes,such as ubiquitin-mediated proteolysis and human immunodeficiency virus 1 infection.CONCLUSION In conclusion,this study systematically explored the significance of COPS6 across different tumor types,providing a solid foundation for considering COPS6 as a novel biomarker in cancer research.
文摘COPS(Concurrent Official Production System)是将产生式系统程序设计语言OPS5和面向进程的程序设计范例相结合,而设计的用于支持协同式问题求解的程序系统。本文详细阐述了COPS通讯原语的设计及其对应的语法和语义。COPS不仅能在单机上实现,而且也可以直接的移植到分布式系统上。
文摘背景与目的:卵巢癌是妇科恶性肿瘤中发病率排名第3、致死率排名第1的疾病。卵巢癌的预后很差,这是由于大部分患者确诊时已处于晚期并且对铂类药物化疗易耐药,异常的DNA修复是导致铂类耐药的重要原因,针对性地干扰DNA损伤修复相关分子可能是提高铂类药物化疗敏感性的新手段。Ku86是参与非同源末端连接(non-homologous end joining,NHEJ)过程的一个关键分子,能够有效地修复DNA双链断裂(DNA double-strand break,DSB)。方法:使用免疫组织化学染色和细胞免疫荧光法检测Ku86在卵巢癌组织和细胞中的定位。使用RNAi技术下调Ku86,用顺铂处理后使用流式细胞术检测凋亡,使用细胞计数试剂盒(cell counting kit-8,CCK-8)法检测IC_(50)。使用蛋白质印迹法(Western blot)分别检测并分析Ku86与拓扑异构酶Ⅰ(topoisomeraseⅠ,TOP1)及COP9信号复合体的第5种成分(the fifth component of the COP9 signalosome,COPS5)的关系。结果:下调Ku86可以减少由于顺铂引起的细胞凋亡,降低卵巢癌细胞对于顺铂的敏感性。Ku86对顺铂在卵巢癌药物敏感性中的这种影响可能是通过调节TOP1和COPS5发挥作用的。结论:TOP1和COPS5都是与DNA损伤修复有关并能影响铂类药物化疗敏感性的重要分子,下调Ku86会使TOP1的表达增高和COPS5的表达减少。卵巢癌中与铂类药物敏感性有关的生物标志物之间有相关性,靶向Ku86的治疗可能是提高卵巢癌药物敏感性的有效策略。
