Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from...Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.展开更多
利用电镜和酶联免疫法在云南省采集到的5份南瓜病样中检测到番木瓜环斑病毒（Papaya ring spot virus，PRSV）。为了进一步从分子水平确定云南省南瓜病毒病原种类，并为下一步转基因育种提供抗性基因，采用反转录PCR（RT—PCR）方法扩增...利用电镜和酶联免疫法在云南省采集到的5份南瓜病样中检测到番木瓜环斑病毒（Papaya ring spot virus，PRSV）。为了进一步从分子水平确定云南省南瓜病毒病原种类，并为下一步转基因育种提供抗性基因，采用反转录PCR（RT—PCR）方法扩增了5个分离物的外壳蛋白（coat protein，CP）基因片段，并克隆到pGEM—T载体中。核苷酸序列测定表明，番木瓜环斑病毒石屏分离物（PRSV—SP）和番木瓜环斑病毒蒙自分离物（PRSV—MZ）的CP基因长873nt，编码290个氨基酸，番木瓜环斑病毒峨山分离物（PRSV—ES）、番木瓜环斑病毒版纳分离物（PRSV—BN）和番木瓜环斑病毒宾川分离物（PRSV—BC），3个分离物CP基因长867nt，编码288个氨基酸。PRSV5个分离物核苷酸序列的同源性在94％以上，氨基酸序列的同源性在96％以上。与国内外17个分离物相比，核苷酸序列同源性为89．6％～98．7％，氨基酸序列同源性为86．5％～99．6％。其中PRSV—SP和来自于越南分离物PRSV—V47无论是核苷酸序列，还是氨基酸序列同源性都达到了最高，而5个分离物与来自于巴西（PRSV—BR）、美国（PRSV—USA）、墨西哥（PRSV—Y）核苷酸序列同源性均低于90％。展开更多
文摘Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.
文摘利用电镜和酶联免疫法在云南省采集到的5份南瓜病样中检测到番木瓜环斑病毒（Papaya ring spot virus，PRSV）。为了进一步从分子水平确定云南省南瓜病毒病原种类，并为下一步转基因育种提供抗性基因，采用反转录PCR（RT—PCR）方法扩增了5个分离物的外壳蛋白（coat protein，CP）基因片段，并克隆到pGEM—T载体中。核苷酸序列测定表明，番木瓜环斑病毒石屏分离物（PRSV—SP）和番木瓜环斑病毒蒙自分离物（PRSV—MZ）的CP基因长873nt，编码290个氨基酸，番木瓜环斑病毒峨山分离物（PRSV—ES）、番木瓜环斑病毒版纳分离物（PRSV—BN）和番木瓜环斑病毒宾川分离物（PRSV—BC），3个分离物CP基因长867nt，编码288个氨基酸。PRSV5个分离物核苷酸序列的同源性在94％以上，氨基酸序列的同源性在96％以上。与国内外17个分离物相比，核苷酸序列同源性为89．6％～98．7％，氨基酸序列同源性为86．5％～99．6％。其中PRSV—SP和来自于越南分离物PRSV—V47无论是核苷酸序列，还是氨基酸序列同源性都达到了最高，而5个分离物与来自于巴西（PRSV—BR）、美国（PRSV—USA）、墨西哥（PRSV—Y）核苷酸序列同源性均低于90％。
