The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidiumparvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. Theeukaryotic expressing vector pcDNA3-15/60 wa...The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidiumparvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. Theeukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene intopcDNA3 (+) in XhoⅠand EcoRⅠ. A vaccination protocol was the adult pregnant goatsinoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infectedwith C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce theimmune response of goats and the vaccinated goats can transfer the immunity to offspringconferring protection against C.parvum infection. These suggested that the recombinantplasmid could be a DNA vaccine candidate.展开更多
基金supported by Natural Science Foundation of Jilin Province for Outstanding Young scientists Award,China(2002).
文摘The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidiumparvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. Theeukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene intopcDNA3 (+) in XhoⅠand EcoRⅠ. A vaccination protocol was the adult pregnant goatsinoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infectedwith C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce theimmune response of goats and the vaccinated goats can transfer the immunity to offspringconferring protection against C.parvum infection. These suggested that the recombinantplasmid could be a DNA vaccine candidate.
