马钢第一钢轧总厂冷轧区域现有酸洗连轧、1#镀锌、2#镀锌3条连续生产线。每条生产线全长均超过300m(不计活套量),为防止受生产线较长等其他因素影响带钢在生产线上的位置,分别采用了EMG、NIRECO、FIFE的CPC(Center Position Control)系...马钢第一钢轧总厂冷轧区域现有酸洗连轧、1#镀锌、2#镀锌3条连续生产线。每条生产线全长均超过300m(不计活套量),为防止受生产线较长等其他因素影响带钢在生产线上的位置,分别采用了EMG、NIRECO、FIFE的CPC(Center Position Control)系统。阐述CPC系统的构成、控制原理、工作原理以及各家产品在马钢冷轧生产线上的应用。展开更多
Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecul...Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.展开更多
文摘马钢第一钢轧总厂冷轧区域现有酸洗连轧、1#镀锌、2#镀锌3条连续生产线。每条生产线全长均超过300m(不计活套量),为防止受生产线较长等其他因素影响带钢在生产线上的位置,分别采用了EMG、NIRECO、FIFE的CPC(Center Position Control)系统。阐述CPC系统的构成、控制原理、工作原理以及各家产品在马钢冷轧生产线上的应用。
基金supported by the National Science and Technology Major Project of China (2008ZX08001-004)
文摘Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.
