In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of...In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of induced fission tracks. The results show that, the uranium concentrations ranged from 4.61 ppm, 56.93 Bq/kg （AL-Falluja AL-Jolan District） to 1.81 ppm 22.35 Bq/kg （Anah）with an average 3.42±0.79 ppm, 41.76±9.04 Bq/kg. The present results have shown that the uranium concentrations in the studied surface soil samples were less than the allowed limit 11.7 ppm recommended by UNSCEAR, 1993.展开更多
[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,us...[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.展开更多
The aim of the study was to compare radon concentrations and effective dose equivalent in state hospitals inside the Erbil city having different periods of building. Effective doses were estimated in different floors ...The aim of the study was to compare radon concentrations and effective dose equivalent in state hospitals inside the Erbil city having different periods of building. Effective doses were estimated in different floors of the five selected hospitals by using the （CR-39） solid state nuclear track detectors with the guide of 1CRP Publication 66. The hanged dosimeters were left for about 60 days and after etching the radon concentration, annual effective dose and effective dose equivalents were calculated. Annual effective doses were estimated as 〈 1 mSv, so the results reveal the present concentrations to be below the standard values in the studied hospitals. Therefore, no healthy risks concerning the radon activities are existing in the hospital staff and patients in these hospitals.展开更多
文摘In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of induced fission tracks. The results show that, the uranium concentrations ranged from 4.61 ppm, 56.93 Bq/kg （AL-Falluja AL-Jolan District） to 1.81 ppm 22.35 Bq/kg （Anah）with an average 3.42±0.79 ppm, 41.76±9.04 Bq/kg. The present results have shown that the uranium concentrations in the studied surface soil samples were less than the allowed limit 11.7 ppm recommended by UNSCEAR, 1993.
基金Supported by Guizhou International Cooperation Project on Science and Technology[(2013)7040]the 20th Project of the Joint Committee on Scientific and Technical Cooperation between the Government of the Kingdom of Thailand and the Government of the People’s Republic of China (20-606J)the Fund from Suranaree University of Technology,Thailand (SUT3-304-54-12-29)
文摘[Objective] This study aimed to establish a quantitative real-time PCR （qRT-PCR） system for detecting the expression of rice beta-glucosidase gene Os1bglu4.[Method] The PCR was conducted with SYBR Green Ⅰ method,using the primers of reference gene actin or ubiquitin.[Result] Actin was more suitable to be the reference gene than ubiquitin.More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results,so,0.4 μmol/L was selected as the optimal primer concentration in this study.The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃,therefore,annealing temperature was set at 60 ℃.Compared with the reaction system of 25 μl,the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So,the 10 μl reaction system was selected,which significantly reduces the research costs for the detection of a large amount of samples in future study.
文摘The aim of the study was to compare radon concentrations and effective dose equivalent in state hospitals inside the Erbil city having different periods of building. Effective doses were estimated in different floors of the five selected hospitals by using the （CR-39） solid state nuclear track detectors with the guide of 1CRP Publication 66. The hanged dosimeters were left for about 60 days and after etching the radon concentration, annual effective dose and effective dose equivalents were calculated. Annual effective doses were estimated as 〈 1 mSv, so the results reveal the present concentrations to be below the standard values in the studied hospitals. Therefore, no healthy risks concerning the radon activities are existing in the hospital staff and patients in these hospitals.
