Marker free is a rapidly developed strategy that offers a new approach for the elimination of public concerns caused by the selectable marker genes conferring antibiotic or herbicide resistance and so on. Furthermore,...Marker free is a rapidly developed strategy that offers a new approach for the elimination of public concerns caused by the selectable marker genes conferring antibiotic or herbicide resistance and so on. Furthermore, marker_free transgenic plants (MFTPs) have a number of special advantages, such as decreasing the concerns about safety of selectable marker and stacking transgenes progressively into transgenic plants, which significantly owns potential application value. Major approaches developed recently for obtaining MFTPs were reviewed in this paper.展开更多
cAMP反应元件结合蛋白（cAMP-responsive element bindingprotein，CREB）作为转录因子，促进启动子中具有cAMP反应元件（cAMPresponseelement，CRE）的基因转录。近年的研究显示，CREB转录共激活因子（CREB-regulated transcription co...cAMP反应元件结合蛋白（cAMP-responsive element bindingprotein，CREB）作为转录因子，促进启动子中具有cAMP反应元件（cAMPresponseelement，CRE）的基因转录。近年的研究显示，CREB转录共激活因子（CREB-regulated transcription coactivator,CRTC；又名transducer of regulated CREB，TORC）家族可以显著增强CREB靶基因的转录。CRTC家族有3个成员（CRTC1-3），其中CRTC1在脑组织中高表达。一些研究表明CRTC1在神经元树突生长发育、长时程突触可塑性、记忆巩固和再巩固等过程中发挥重要作用；且在神经退行性相关疾病等发病过程中出现表达或活性异常。本文主要针对CRTC1表达或活性异常在神经退行性相关疾病发病过程中的作用及分子机制做一综述。展开更多
The efforts of many laboratories worldwide and large scale international mutagenesis consortia have resulted in mutagenesis of nearly all mouse genes, and present efforts are devoted to large scale phenotyping of germ...The efforts of many laboratories worldwide and large scale international mutagenesis consortia have resulted in mutagenesis of nearly all mouse genes, and present efforts are devoted to large scale phenotyping of germline null mutant mice (Ayadi et al., 2012). Although early lethality associated with loss of function of a given gene may prevent elucidating its function at a later stage or in a given tissue, conditional alleles in which a critical part of the gene is flanked by recombinase target sites provide an opportunity for addressing gene function at later stages (Branda and Dymecki,2004). Fortunately, many of the mutant alleles generated by the consortia allow for conditional mutagenesis using site directed recombinases.展开更多
文摘Marker free is a rapidly developed strategy that offers a new approach for the elimination of public concerns caused by the selectable marker genes conferring antibiotic or herbicide resistance and so on. Furthermore, marker_free transgenic plants (MFTPs) have a number of special advantages, such as decreasing the concerns about safety of selectable marker and stacking transgenes progressively into transgenic plants, which significantly owns potential application value. Major approaches developed recently for obtaining MFTPs were reviewed in this paper.
基金supported by the National Natural Science Foundation of China(No.81171036,81371224)the Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University,China(No.G7)
文摘cAMP反应元件结合蛋白（cAMP-responsive element bindingprotein，CREB）作为转录因子，促进启动子中具有cAMP反应元件（cAMPresponseelement，CRE）的基因转录。近年的研究显示，CREB转录共激活因子（CREB-regulated transcription coactivator,CRTC；又名transducer of regulated CREB，TORC）家族可以显著增强CREB靶基因的转录。CRTC家族有3个成员（CRTC1-3），其中CRTC1在脑组织中高表达。一些研究表明CRTC1在神经元树突生长发育、长时程突触可塑性、记忆巩固和再巩固等过程中发挥重要作用；且在神经退行性相关疾病等发病过程中出现表达或活性异常。本文主要针对CRTC1表达或活性异常在神经退行性相关疾病发病过程中的作用及分子机制做一综述。
基金supported by grants RO1 DE022363 and RO1 DE022778 from the National Institute of Dental and Craniofacial Research
文摘The efforts of many laboratories worldwide and large scale international mutagenesis consortia have resulted in mutagenesis of nearly all mouse genes, and present efforts are devoted to large scale phenotyping of germline null mutant mice (Ayadi et al., 2012). Although early lethality associated with loss of function of a given gene may prevent elucidating its function at a later stage or in a given tissue, conditional alleles in which a critical part of the gene is flanked by recombinase target sites provide an opportunity for addressing gene function at later stages (Branda and Dymecki,2004). Fortunately, many of the mutant alleles generated by the consortia allow for conditional mutagenesis using site directed recombinases.
