From the bulbs of Crinum asiaticum L. var. sinicum Bak., a new alkaloid, crinisine, has been isolated and characterized on the basis of comprehensive spectral analyses. Additionally, three known alkaloids, lycorine,...From the bulbs of Crinum asiaticum L. var. sinicum Bak., a new alkaloid, crinisine, has been isolated and characterized on the basis of comprehensive spectral analyses. Additionally, three known alkaloids, lycorine, powelline and ermine were also obtained.展开更多
A new phenolic compound was isolated from the ethanol extract of the bulbs of Crinum asiaticum L.var.sinicum Baker.Its structure was defined as 1-（2-hydroxy-4-hydroxymethyl）phenyl-6-O-caffeoyl-β-D-gluco-pyranoside ...A new phenolic compound was isolated from the ethanol extract of the bulbs of Crinum asiaticum L.var.sinicum Baker.Its structure was defined as 1-（2-hydroxy-4-hydroxymethyl）phenyl-6-O-caffeoyl-β-D-gluco-pyranoside on the basis of spectroscopic evidences.展开更多
Crinasiadine 1 has been easily synthesized by palladium acetate catalyzed cyclization of N- (o-bromophenyl) -3, 4-metnylenedioxybenzamide.The synthesis trisphearidine 8 with a practical type of palladium chloride c...Crinasiadine 1 has been easily synthesized by palladium acetate catalyzed cyclization of N- (o-bromophenyl) -3, 4-metnylenedioxybenzamide.The synthesis trisphearidine 8 with a practical type of palladium chloride catalyzed cyclization as the key step is reported展开更多
目的运用网络药理学筛选文殊兰的主要活性成分及其靶点,探究文殊兰抗肿瘤的潜在作用机制。方法通过TCMID数据库检索文殊兰的化学成分,再经Batman与Swisstargets并结合相关文献筛选出文殊兰的主要活性成分及其潜在靶点,建立靶点数据;通过...目的运用网络药理学筛选文殊兰的主要活性成分及其靶点,探究文殊兰抗肿瘤的潜在作用机制。方法通过TCMID数据库检索文殊兰的化学成分,再经Batman与Swisstargets并结合相关文献筛选出文殊兰的主要活性成分及其潜在靶点,建立靶点数据;通过Gene Cards、NCBI数据库筛选出肿瘤相关靶点;用Cytoscape3.6.3软件构建"文殊兰-成分-肿瘤-靶点"网络可视化关系图;以蛋白互作网络分析数据库,确立文殊兰和肿瘤的蛋白互作网络;利用基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)数据库对相关靶点进行富集分析,了解文殊兰抗肿瘤可能的生物过程及其通路;利用分子对接进行半柔性对接反向验证。结果从文殊兰中筛选出15种活性成分,与肿瘤有关的通路有25个,文殊兰抗肿瘤主要作用于INS、GAPDH、VEGFA、EGFR、TNF等靶蛋白,KEGG通路富集结果显示,文殊兰主要作用于prostate cancer、proteoglycans in cancer、microRNAs in cancer、viral carcinogenesis等信号通路。结论文殊兰可能对前列腺癌、非小细胞癌、胰腺癌、膀胱癌、乳腺癌等具有一定的治疗作用,诱导肿瘤细胞凋亡是文殊兰成分抗肿瘤的一个重要机制。初步推测并验证了文殊兰抗肿瘤的主要靶基因和通路,为后续进一步探讨文殊兰靶向抑制肿瘤细胞提供新思路。展开更多
文摘From the bulbs of Crinum asiaticum L. var. sinicum Bak., a new alkaloid, crinisine, has been isolated and characterized on the basis of comprehensive spectral analyses. Additionally, three known alkaloids, lycorine, powelline and ermine were also obtained.
基金supported by program for Changjiang Scholars and Innovative Research Team in University(PCSIRT),the National Natural Science Foundation of China(No.20402024)the Scientific Foundation of Shanghai of China(No.05DZ19733,06DZ19717,and 06DZ19005).
文摘A new phenolic compound was isolated from the ethanol extract of the bulbs of Crinum asiaticum L.var.sinicum Baker.Its structure was defined as 1-（2-hydroxy-4-hydroxymethyl）phenyl-6-O-caffeoyl-β-D-gluco-pyranoside on the basis of spectroscopic evidences.
文摘Crinasiadine 1 has been easily synthesized by palladium acetate catalyzed cyclization of N- (o-bromophenyl) -3, 4-metnylenedioxybenzamide.The synthesis trisphearidine 8 with a practical type of palladium chloride catalyzed cyclization as the key step is reported
文摘目的运用网络药理学筛选文殊兰的主要活性成分及其靶点,探究文殊兰抗肿瘤的潜在作用机制。方法通过TCMID数据库检索文殊兰的化学成分,再经Batman与Swisstargets并结合相关文献筛选出文殊兰的主要活性成分及其潜在靶点,建立靶点数据;通过Gene Cards、NCBI数据库筛选出肿瘤相关靶点;用Cytoscape3.6.3软件构建"文殊兰-成分-肿瘤-靶点"网络可视化关系图;以蛋白互作网络分析数据库,确立文殊兰和肿瘤的蛋白互作网络;利用基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)数据库对相关靶点进行富集分析,了解文殊兰抗肿瘤可能的生物过程及其通路;利用分子对接进行半柔性对接反向验证。结果从文殊兰中筛选出15种活性成分,与肿瘤有关的通路有25个,文殊兰抗肿瘤主要作用于INS、GAPDH、VEGFA、EGFR、TNF等靶蛋白,KEGG通路富集结果显示,文殊兰主要作用于prostate cancer、proteoglycans in cancer、microRNAs in cancer、viral carcinogenesis等信号通路。结论文殊兰可能对前列腺癌、非小细胞癌、胰腺癌、膀胱癌、乳腺癌等具有一定的治疗作用,诱导肿瘤细胞凋亡是文殊兰成分抗肿瘤的一个重要机制。初步推测并验证了文殊兰抗肿瘤的主要靶基因和通路,为后续进一步探讨文殊兰靶向抑制肿瘤细胞提供新思路。
