Based on RNA sequences using transcriptome analysis, 37 new simple sequence repeat (SSR) primer sets were developed for Perilla species. These new SSR markers were applied to analyze the genetic diversity among 15 acc...Based on RNA sequences using transcriptome analysis, 37 new simple sequence repeat (SSR) primer sets were developed for Perilla species. These new SSR markers were applied to analyze the genetic diversity among 15 accessions of Perilla species. A total of 182 alleles were confirmed in 37 loci, with an average of 4.9 alleles per locus and from 2 to 9 alleles per locus. The MAF (major allele frequency) per locus varied from 0.200 to 0.733, with an average of 0.463. The gene diversity (GD) ranged from 0.391 to 0.853, with an average of 0.670. The average polymorphic information content (PIC) was 0.624, ranging from 0.315 to 0.838. The new SSR markers of Perilla species reported in this study may provide potential markers to analyze the genetic diversity and genetic relationships of Perilla species. In addition, new Perilla SSR markers developed from transcriptome analysis can be useful for the identification of cultivars, conservation of Perilla germplasm resources, and genetic mapping and designating of important genes/QTLs for future Perilla crop breeding programs.展开更多
特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位...特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位、遗传多样性分析和辅助全基因组测序等方面的应用,讨论了其应用存在的问题,展望了其发展前景。展开更多
文摘Based on RNA sequences using transcriptome analysis, 37 new simple sequence repeat (SSR) primer sets were developed for Perilla species. These new SSR markers were applied to analyze the genetic diversity among 15 accessions of Perilla species. A total of 182 alleles were confirmed in 37 loci, with an average of 4.9 alleles per locus and from 2 to 9 alleles per locus. The MAF (major allele frequency) per locus varied from 0.200 to 0.733, with an average of 0.463. The gene diversity (GD) ranged from 0.391 to 0.853, with an average of 0.670. The average polymorphic information content (PIC) was 0.624, ranging from 0.315 to 0.838. The new SSR markers of Perilla species reported in this study may provide potential markers to analyze the genetic diversity and genetic relationships of Perilla species. In addition, new Perilla SSR markers developed from transcriptome analysis can be useful for the identification of cultivars, conservation of Perilla germplasm resources, and genetic mapping and designating of important genes/QTLs for future Perilla crop breeding programs.
文摘特异长度扩增片段测序(specific length amplified fragment sequencing,SLAF-seq)技术是以第二代测序为基础的一种简化基因组测序技术。本研究简述了SLAF-seq技术流程,综述了该技术在蔬菜分子标记开发、高密度遗传图谱构建、基因定位、遗传多样性分析和辅助全基因组测序等方面的应用,讨论了其应用存在的问题,展望了其发展前景。
