急性心肌梗死患者血清CSF2RA、MCP-1、GDF-15水平变化及其与病情的相关性

目的:探究急性心肌梗死患者血清中炎症细胞因子粒巨噬细胞集落刺激因子受体α(CSF2RA)、单核细胞趋化蛋白-1(MCP-1)、生长分化因子15(GDF-15)水平变化及其与病情的相关性。方法:选取106例急性心肌梗死患者作为研究组,并按照冠脉病变支数分为单支组(n=28)、双支组(n=41)和三支组(n=37);另选100例同期体检健康者作为对照组。酶联免疫吸附法(ELISA)检测研究组和对照组血清CSF2RA、MCP-1、GDF-15、肌钙蛋白(cTn)和肌酸激脢(CK-MB)表达水平,冠脉病变程度评价采用Gensini评分,并对血清中CSF2RA、MCP-1、GDF-15、cTn、CK-MB水平和Gensini评分进行相关性分析。结果:研究组Gensini评分、cTn、CK-MB、CSF2RA、MCP-1、GDF-15水平均高于对照组(均P<0.05)。与单支组相比,双支组、三支组Gensini评分、cTn、CK-MB、CSF2RA、MCP-1、GDF-15水平增加(P<0.05);与双支组相比,三支组Gensini评分、cTn、CK-MB、CSF2RA、MCP-1、GDF-15水平增加(P<0.05)。研究组中CSF2RA、MCP-1、GDF-15与cTn、CK-MB、Gensini评分均呈正相关(P<0.05)。以CSF2RA、MCP-1、GDF-15、cTn和CK-MB为变量评估冠脉病变严重程度的敏感度分别为85.7%、82.1%、76.8%、98.2%和89.3%,特异度分别为74.0%、72.0%、74.0%、58.0%和62.0%,受试者工作特征曲线(ROC)下面积(AUC)分别为0.864、0.837、0.817、0.835和0.810,评估价值CSF2RA>MCP-1>cTn>GDF-15>CK-MB(P<0.05)。结论:急性心肌梗死患者血清CSF2RA、MCP-1、GDF-15水平与心肌损伤程度及冠脉病变严重程度密切相关,可作为评估急性心肌梗死病情严重程度的指标之一。

Expression and Clinical Value Analysis of CSF2RA in Malignant Tumor Based on Database

Objective: to explore the expression and clinical value of CSF2RA in pan-cancer. Methods: the data was extracted from ONCOMINE, Human Protein Atlas and Kaplan Meier-plotter. The expression level of CSF2RA on cancer tissues and normal tissues, the relationship between CSF2RA and overall survival of cancer patients were analyzed, respectively. Results: CSF2RA mRNA was over-expressed on breast cancer, colorectal cancer, kidney cancer and liver cancer, and CSF2RA protein was over-expressed on melanoma. The expression level of CSF2RA was not associated with overall survival of cancer patients significantly. Conclusion: CSF2RA is over-expression on certain cancers and most immune cells, which maybe contributes to activation of immune system. The high expression of CSF2RA protein on melanoma is maybe associated with adverse outcome of application of GM-CSF.

CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway

Recent studies have demonstrated that cancer-associated adipocytes (CAAs) in the tumor microenvironment are involved in the malignant progression of breast cancer. However, the underlying mechanism of CAA formation and its effects on the development of breast cancer are still unknown. Here, we show that CSF2 is highly expressed in both CAAs and breast cancer cells. CSF2 promotes inflammatory phenotypic changes of adipocytes through the Stat3 signaling pathway, leading to the secretion of multiple cytokines and proteases, particularly C–X–C motif chemokine ligand 3 (CXCL3). Adipocyte-derived CXCL3 binds to its specific receptor CXCR2 on breast cancer cells and activates the FAK pathway, enhancing the mesenchymal phenotype, migration, and invasion of breast cancer cells. In addition, a combination treatment targeting CSF2 and CXCR2 shows a synergistic inhibitory effect on adipocyte-induced lung metastasis of mouse 4T1 cells in vivo. These findings elucidate a novel mechanism of breast cancer metastasis and provide a potential therapeutic strategy for breast cancer metastasis.

ZrB_2-SiC和C_(sf)/ZrB_2-SiC超高温陶瓷基复合材料烧蚀机理的研究

通过真空热压工艺制备了ZrB_2-SiC材料和C^(sf)(碳短纤维)/ZrB_2-SiC超高温陶瓷基复合材料.采用氧乙炔火焰在4186.8kW/m^2的热流下分别喷吹烧蚀两种材料180s.ZrB_2-SiC材料表面最高温度达到2406℃,烧蚀后质量烧蚀率为-0.14%,线烧蚀率为-1×10^(-3)mm/s,C_(sf)/ZrB_2-SiC材料表面最高温度达到1883℃,烧蚀后质量烧蚀率为-0.19%,线烧蚀率为-4×10^(-4)mm/s.对两种材料烧蚀表面和剖面的分析发现,ZrB_2-SiC材料烧蚀后由表及里依次形成了疏松ZrO_2氧化层、SiC富集层和未反应层的三层结构,其中SiC富集层能够起到抗氧化的作用.C_(sf)/ZrB_2-SiC材料烧蚀后由外到内分别形成了ZrO_2-SiO_2氧化层、SiC耗尽层和未反应层的三层结构,其中最外层以ZrO_2为骨架,SiO_2弥合其中的结构有效地阻挡了烧蚀中氧的侵入.

Molecular Cloning, Characterization and Expression Analysis of Macrohage-Colony Stimulating Factor 2 Gene from Grass Carp (<i>Ctenopharyngodon idellus</i>)

Macrophage colony-stimulating factor (CSF-1/M-CSF) is a key factor for the differentiation, growth and survival of monocytes/macrophages and osteoclasts. The functions of M-CSF have been well characterized in mammals. In this study, we have cloned and sequenced the cDNA of M-CSF2 in grass carp. The grass carp M-CSF2 cDNA was 1487 bp in length, containing an open reading frame of 855 bp that encodes 284 amino acids. The deduced protein of grass carp M-CSF2 possessed same domains similar to its mammalian counterparts. Multiple alignments and phylogenetic tree indicate that the grass carp M-CSF2 exhibits close evolutionary relationship with its counterparts in other teleosts. Lastly, the tissue distribution results also showed that the grass carp M-CSF2 transcript was dominantly expressed in head kidney, kidney and spleen in vivo.

短切碳纤维含量对C_(sf)/Al_2O_3复合材料性能的影响

采用热压烧结法制备了短切碳纤维-氧化铝(Csf/Al2O3)复合材料,研究了短切碳纤维含量对该复合材料维氏硬度、抗弯强度及微波介电性能的影响。结果表明,热压烧结可以获得高致密度、高硬度的Csf/Al2O3复合材料。碳纤维的加入降低了氧化铝基体的抗弯强度,但随着碳纤维含量的增加,抗弯强度有增大的趋势,Csf/Al2O3复合材料复介电常数的实部与虚部也显著提高。

小儿结核性脑膜炎脑脊液β_2微球蛋白测定的临床意义

本文用放免法测定146例CSFβ_2m,结果小儿结脑组为2.2±1.1mg/L,明显高于正常对照组、散脑组和GBS组,但与化脑组相比无显著差异,动态观察其变化与病情平行,提示测定CSFβ_2m对小儿结脑诊断及观察病情有一定参考意义。

MH60.CSF_2细胞株对IL-6依赖性变异的观察

IL-6依赖性MH60.CSF_2细胞株用於测定淋巴细胞培养上清中IL-6生物活性具有灵敏度高、方便易行等优点,但该株细胞在传代培养中易逐渐丧失对IL-6的依赖性。我们将此株细胞亚克隆后,动态观察各代细胞对IL-6依赖性的变化,确定其用於IL-6测定的最好代数、细胞用量和培养时间。现报告如下。1 材料和方法 材料:MH60.CSF_2细胞株由山东省医科院肿瘤生物治疗研究中心田志刚教授惠赠。小鼠rIL-6标准品购自德国Bochringer公司。

LDH、CSFβ2-MG及中性粒细胞CD64对老年患者中枢神经系统感染诊断价值

目的检测老年患者中枢神经系统感染情况,并监测LDH、CSFβ2-MG及中性粒细胞CD64临床指标,以指导临床疾病的诊断。方法收集103例本院老年患者临床资料,检测患者感染细菌及病毒情况。脑脊液及外周血样本检测LDH、CSFβ2-MG及中性粒细胞CD64临床指标。采用t检验进行统计学分析。结果 23例中枢神经系统细菌感染患者检出的27株细菌主要包括13株鲍曼不动杆菌、8株肺炎克雷伯菌、2株表皮葡萄球菌以及4株其他病原菌,构成比分别为48.15%、29.63%、7.41%和14.81%;21例病毒感染患者检出的24株病毒主要包括11株肠道病毒、9株单纯疱疹病毒、4株其他病毒,构成比分别为45.83%、37.50%和16.67%。两组患者年龄、病程等基础临床资料差异无统计学意义。细菌感染组患者LDH、CSFβ2-MG以及中性粒细胞CD64水平显著高于未感染组患者。病毒感染组患者LDH、CSFβ2-MG以及中性粒细胞CD64水平显著高于未感染组患者。结论老年患者中枢神经系统感染细菌以鲍曼不动杆菌为主,感染病毒以肠道病毒为主。LDH、CSFβ2-MG以及中性粒细胞CD64临床指标监测对老年患者感染情况及疾病诊断具有重要临床价值。