Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power ce...Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular dynamics.However,how these free MT tips are stabilized remains poorly understood.Here,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static MTs.Real-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly suppressed.Consistently,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more dynamic.This CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting polymerization.Importantly,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated depolymerization.These findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.展开更多
为有效缓解三北地区高比例可再生能源并网所存在的大规模弃风问题,将火电机组、热电联产机组、光热电站、风电厂和电热负荷聚合为虚拟电厂。首先采用场景分析法对日前风光场景进行随机优化,降低风光预测误差;利用综合需求响应机制充分...为有效缓解三北地区高比例可再生能源并网所存在的大规模弃风问题,将火电机组、热电联产机组、光热电站、风电厂和电热负荷聚合为虚拟电厂。首先采用场景分析法对日前风光场景进行随机优化,降低风光预测误差;利用综合需求响应机制充分调动电热柔性负荷资源。然后综合考虑系统运行成本、弃风成本和需求响应成本构建虚拟电厂(virtual power plant,VPP)系统日前调度优化模型,并在多种运行模式下求解以获得最优日前调度计划。最后通过算例分析验证所提方案在提高系统经济性的同时能有效促进风电消纳。展开更多
基金supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,91854203,31621002,2017YFA0503600,21922706,and 92153302 to Xing Liu,2022YFA1302700 to Z.W.,32100612 to Xu Liu)the Ministry of Education of China(IRT_17R102,20113402130010,and YD2070006001 to Xing Liu)+3 种基金the Plans for Major Provincial Science&Technology Projects of Anhui Province(202303a0702003 to Xing Liu)the Fundamental Research Funds for the Central Universities(KB9100000007 and KB9100000013 to Xing Liu)University of Science and Technology of China Start-up Fund(KY9990000167 to Z.W.)Zhejiang Provincial Natural Science Foundation(LY23C070002 to W.W.)。
文摘Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular dynamics.However,how these free MT tips are stabilized remains poorly understood.Here,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static MTs.Real-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly suppressed.Consistently,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more dynamic.This CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting polymerization.Importantly,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated depolymerization.These findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.
文摘为有效缓解三北地区高比例可再生能源并网所存在的大规模弃风问题,将火电机组、热电联产机组、光热电站、风电厂和电热负荷聚合为虚拟电厂。首先采用场景分析法对日前风光场景进行随机优化,降低风光预测误差;利用综合需求响应机制充分调动电热柔性负荷资源。然后综合考虑系统运行成本、弃风成本和需求响应成本构建虚拟电厂(virtual power plant,VPP)系统日前调度优化模型,并在多种运行模式下求解以获得最优日前调度计划。最后通过算例分析验证所提方案在提高系统经济性的同时能有效促进风电消纳。
