目的:探讨CUG连接蛋白1(CUG-binding protein 1,CUGBP1)在膀胱癌组织中的表达及其表达对膀胱癌BIU-87细胞株增殖及迁移能力的影响。方法:采用免疫组化及Western blot检测65例膀胱癌及相应的癌旁正常组织中CUGBP1的表达,并探讨CUGBP1表...目的:探讨CUG连接蛋白1(CUG-binding protein 1,CUGBP1)在膀胱癌组织中的表达及其表达对膀胱癌BIU-87细胞株增殖及迁移能力的影响。方法:采用免疫组化及Western blot检测65例膀胱癌及相应的癌旁正常组织中CUGBP1的表达,并探讨CUGBP1表达与膀胱癌患者临床病理特征的关系。同时,采用siR NA特性干扰片段下调CUGBP1在膀胱癌细胞株BIU-87中的表达,探讨CUGBP1下调对细胞增殖及侵袭能力的影响。结果:免疫组化结果显示CUGBP1在膀胱癌组织中的阳性表达率为58.5%,高于癌旁正常组织(9.2%),差异具有统计学意义(P<0.01);Western blot结果也显示CUGBP1在膀胱癌组织中的表达高于癌旁正常组织。同时,CUGBP1在膀胱癌组织中的表达与淋巴结转移、TNM分期及分化程度有关(P<0.05)。此外,BIU-87细胞株中CUGBP1表达明显下调后,细胞的增殖及侵袭能力均明显下降(P<0.05)。结论:CUGBP1表达与膀胱癌的形成相关,其表达下调可明显抑制细胞增殖及侵袭。展开更多
Streptococcus mutans is a well-known cause of dental caries,due to its acidogenicity,aciduricity,and ability to synthesize exopolysaccharides in dental plaques.Intriguingly,not all children who carry S.mutans manifest...Streptococcus mutans is a well-known cause of dental caries,due to its acidogenicity,aciduricity,and ability to synthesize exopolysaccharides in dental plaques.Intriguingly,not all children who carry S.mutans manifest caries,even with similar characteristics in oral hygiene,diet,and other environmental factors.This phenomenon suggests that host susceptibility potentially plays a role in the development of dental caries;however,the association between host genetics,S.mutans,and dental caries remains unclear.Therefore,this study examined the influence of host gene-by-S.mutans interaction on dental caries.Genome-wide association analyses were conducted in 709 US children (<13 years old),using the dbGap database acquired from the center for oral health research in appalachia (COHRA) and the Iowa Head Start programmes (GEIRS).A generalized estimating equation was used to examine the gene-by-S.mutans interaction effects on the outcomes (decayed and missing/filled primary teeth due to caries).Sequentially,the COHRA and GEIRS data were used to identify potential interactions and replicate the findings.Three loci at the genes interleukin 32 (IL32),galactokinase 2 (GALK2),and CUGBP,Elav-like family member 4 (CELF4) were linked to S.mutans carriage,and there was a severity of caries at a suggestive significance level among COHRA children (P < 9×10?5),and at a nominal significance level among GEIRS children (P = 0.047–0.001).The genetic risk score that combined the three loci also significantly interacted with S.mutans (P < 0.000 1).Functional analyses indicated that the identified genes are involved in the host immune response,galactose carbohydrate metabolism,and food-rewarding system,which could potentially be used to identify children at high risk for caries and to develop personalized caries prevention strategies.展开更多
基金supported by the National Institute of Dental and Craniofacial Research (NIDCR) grant K23DE027412
文摘Streptococcus mutans is a well-known cause of dental caries,due to its acidogenicity,aciduricity,and ability to synthesize exopolysaccharides in dental plaques.Intriguingly,not all children who carry S.mutans manifest caries,even with similar characteristics in oral hygiene,diet,and other environmental factors.This phenomenon suggests that host susceptibility potentially plays a role in the development of dental caries;however,the association between host genetics,S.mutans,and dental caries remains unclear.Therefore,this study examined the influence of host gene-by-S.mutans interaction on dental caries.Genome-wide association analyses were conducted in 709 US children (<13 years old),using the dbGap database acquired from the center for oral health research in appalachia (COHRA) and the Iowa Head Start programmes (GEIRS).A generalized estimating equation was used to examine the gene-by-S.mutans interaction effects on the outcomes (decayed and missing/filled primary teeth due to caries).Sequentially,the COHRA and GEIRS data were used to identify potential interactions and replicate the findings.Three loci at the genes interleukin 32 (IL32),galactokinase 2 (GALK2),and CUGBP,Elav-like family member 4 (CELF4) were linked to S.mutans carriage,and there was a severity of caries at a suggestive significance level among COHRA children (P < 9×10?5),and at a nominal significance level among GEIRS children (P = 0.047–0.001).The genetic risk score that combined the three loci also significantly interacted with S.mutans (P < 0.000 1).Functional analyses indicated that the identified genes are involved in the host immune response,galactose carbohydrate metabolism,and food-rewarding system,which could potentially be used to identify children at high risk for caries and to develop personalized caries prevention strategies.
