Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three stu...Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three studies i.e. study Ⅰ(normal rats), study Ⅱ(hyperglycaemic rats) and study Ⅲ(hypercholesterolemic rats). Each study was further divided into three groups based on diet i.e.control, functional diet(contained extract of black cumin prepared by using conventional solvent) and nutraceutical diet(contained extract of black cumin prepared by supercritical fluid extraction system).Results: During whole trial, an abating trend was observed in the level of serum cholesterol with maximum reduction(12.8%) in nutraceutical group of study Ⅲ. Low density lipoprotein and triglyceride level was also lowered maximum in study Ⅲ as17.1% and 11.6%, respectively. Whereas, highest decline in glucose level was in nutraceutical group of study Ⅱ as 11.2%.Conclusions: Inclusion of black cumin extracts in diet significantly lowers the occurrence of hyperglycaemia and hypercholesterolaemia. Furthermore, hypoglycaemic and hypocholesterolemic potential of nutraceutical diet is more prominent as compared to functional diet.展开更多
Black cumin (Nigella sativa L.;Family-Ranunculaceae) is an important spice crop. Mature seeds are consumed for edible and medical purposes and also used as a food additive and flavour. Seed of black cumin has great po...Black cumin (Nigella sativa L.;Family-Ranunculaceae) is an important spice crop. Mature seeds are consumed for edible and medical purposes and also used as a food additive and flavour. Seed of black cumin has great potentiality as spice crop due to nutritive and medicinal values. The experiments were carried out at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during 2011 to 2012 to determine optimum planting time for seed production of black cumin. The experiment was two factorials. Factor A: 4 genotypes were V1: Exotic, Iran;V2: BARI kalozira-1;V3: Local, Faridpur and V4: Local, Natore. Factor B: sowing date: D1: 16 October;D2: 1 November;D3: 16 November and D4: 1 December. Therefore, treatment combinations were 16 in total. So, in 3 replications total plot was 48. Result revealed that significantly the highest 2.37 t/ha in V1, followed by V2 (1.96 t/ha). V2 and V3 (1.97 t/ha) were statistically similar and maximum yield was obtained from D2 (2.65 t/ha). In combined effect, maximum yield 3.00 t/ha was obtained in V1D2.. Investigation on time of sowing revealed that performance of black cumin was better in earlier sowings (16 October, 1 November) than later ones. The highest yield (4g plant-1;2.65 tha-1) was obtained when the crop was sown on 1 November. Among the genotypes, the exotic one with sowing in 1 November gave the highest seed yield (4.54 g·plant-1;3.00 t·ha-1).展开更多
Essential oils from newly produced (2017) and previous season crop (2016) of cumin (Cuminum cyminum L.) seeds collected from local farmers in Dongla area, north Sudan were extracted and analyzed to determine the effec...Essential oils from newly produced (2017) and previous season crop (2016) of cumin (Cuminum cyminum L.) seeds collected from local farmers in Dongla area, north Sudan were extracted and analyzed to determine the effect of storage period on volatile oil yield and quality. The essential oil was extracted using hydro distillation method. Identification of the volatile compounds was performed using gas chromatography-mass spectrometry (GC/MS). Results revealed that oil obtained from cumin (Cuminum cyminum L.) was pale yellow. With respect to oil characteristics, the specific gravity, refractive index, acid value, ester value and other quality characters were not significantly different between the stored and newly harvested seeds. Thirty-eight compounds were separated and identified in cumin seeds essential oil in both seasons with some minor differences in some components between 2016 and 2017 samples. Major effective components of cumin seeds volatile oil were cumin alcohol (4-isopropylbenzyl alcohol), cumin aldehydes as 4-Isopropylbenzaldehyd and 7,7-dimethylbicyclo (4.1.0) hept-3-ene-4-carbaldehyde known as carenal, terpinenes as γ-terpinene and α-terpinene (1,4-Cyclohexadiene, 1-methyl-4-(1-methylethyl) and 1,3-cyclohexadine-1-methanol, 4(1-methethyle)-), β-pinene, o-cymene and p-menthadienol in both stored and newly harvested samples. Differences between the samples season wise were very minor with respect to type of volatile components but percentage oil yield was largely decreased by storage period.展开更多
Black cumin (<em>Nigella sativa</em> L.) the highly aggregate valuable medicinal plant was field cultivated for two subsequent seasons (2018, 2019) designed as factorial split plot based on randomized comp...Black cumin (<em>Nigella sativa</em> L.) the highly aggregate valuable medicinal plant was field cultivated for two subsequent seasons (2018, 2019) designed as factorial split plot based on randomized complete block with 3 replications. The main factors 4 elicitors: salicylic acid, (SA) Nano-selenium (NPs), yeast (YS) chitosan (CH) and (E0), control. Whereas, the sub-main plot 4 biofertilizers, dray Moringa leaves extract, (MLE), neem dray leaves extract (NME), humic acid (HA) and traditional (NPK) chemical fertilizer as control. Allied statistical analysis of variance revealed that biotic and abiotic elicitors coincide biofertilizer and NPK chemical fertilizer actuated significant positive impacts, dray seed, seed fixed oil, seed essential oil yield production. Also, significantly amelioration bioactive major fatty acids content of seed fixed oil (linolenic > carvone) dihydrolenoleic > oleic) as well as major terpens content of seed essential oil (P-cymene > thnymoquione > Penine). Consequently, multi-repeating elicitation cod be considered reliable strategy achieve sustainable development for <em>N-sativa</em> under, biotic elicitor coincide biofertilizers that excel abiotic elicitors coincide biofertilizer which excel biotic or abiotic elicitors coincide NPK traditional chemical fertilizer.展开更多
OBJECTIVE: To examine whether a combinative treatment with curcumin enhances the effects of the epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI) gefitinib on cell proliferation, clonogenic capacity...OBJECTIVE: To examine whether a combinative treatment with curcumin enhances the effects of the epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI) gefitinib on cell proliferation, clonogenic capacity and apoptosis in the drug-resistant lung cancer cell line NCI-H1975, and further investigate the molecular mechanisms involved.METHODS: NCI-H1975 cells were treated with curcumin and gefitinib alone or in combination, and cell proliferation, clonogenic capacity and apoptosis were examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, clone forming experiments, and flow cytometry, respectively, while p38, extracellular regulated protein kinase(ERK)1/2, and protein kinase B(AKT)phosphorylation were examined using Western blotting.RESULTS: Compared with the effects of either agent alone, the combination of curcumin and gefitinib had a stronger suppressive effect on proliferation and the clonogenic capacity(P < 0.05), and showed an increased ability to promote apoptosis(P < 0.05) and reduce p38, ERK1/2, and AKT phosphorylation(P < 0.05).CONCLUSION: Co-treatment of curcumin and gefitinib significantly improves the ability of gefitinib to inhibit cell proliferation, suppress the clonogenic capacity and enhance apoptosis in NCI-H1975 cells,and these effects are possibly mediated via a decrease in phosphorylation of proteins in downstream pathways of the epidermal growth factor receptor.展开更多
基金partially supported by Higher Education Commission,Pakistan under Pak-US Science and Technology Cooperation Program Phase IV(Project Grant No.10/01/10-09/30/12)“Establishment of Functional and Nutraceutical Food Research Section at the National Institute of Food Science and Technology,University of Agriculture,Faisalabad,Pakistan”
文摘Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three studies i.e. study Ⅰ(normal rats), study Ⅱ(hyperglycaemic rats) and study Ⅲ(hypercholesterolemic rats). Each study was further divided into three groups based on diet i.e.control, functional diet(contained extract of black cumin prepared by using conventional solvent) and nutraceutical diet(contained extract of black cumin prepared by supercritical fluid extraction system).Results: During whole trial, an abating trend was observed in the level of serum cholesterol with maximum reduction(12.8%) in nutraceutical group of study Ⅲ. Low density lipoprotein and triglyceride level was also lowered maximum in study Ⅲ as17.1% and 11.6%, respectively. Whereas, highest decline in glucose level was in nutraceutical group of study Ⅱ as 11.2%.Conclusions: Inclusion of black cumin extracts in diet significantly lowers the occurrence of hyperglycaemia and hypercholesterolaemia. Furthermore, hypoglycaemic and hypocholesterolemic potential of nutraceutical diet is more prominent as compared to functional diet.
文摘Black cumin (Nigella sativa L.;Family-Ranunculaceae) is an important spice crop. Mature seeds are consumed for edible and medical purposes and also used as a food additive and flavour. Seed of black cumin has great potentiality as spice crop due to nutritive and medicinal values. The experiments were carried out at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during 2011 to 2012 to determine optimum planting time for seed production of black cumin. The experiment was two factorials. Factor A: 4 genotypes were V1: Exotic, Iran;V2: BARI kalozira-1;V3: Local, Faridpur and V4: Local, Natore. Factor B: sowing date: D1: 16 October;D2: 1 November;D3: 16 November and D4: 1 December. Therefore, treatment combinations were 16 in total. So, in 3 replications total plot was 48. Result revealed that significantly the highest 2.37 t/ha in V1, followed by V2 (1.96 t/ha). V2 and V3 (1.97 t/ha) were statistically similar and maximum yield was obtained from D2 (2.65 t/ha). In combined effect, maximum yield 3.00 t/ha was obtained in V1D2.. Investigation on time of sowing revealed that performance of black cumin was better in earlier sowings (16 October, 1 November) than later ones. The highest yield (4g plant-1;2.65 tha-1) was obtained when the crop was sown on 1 November. Among the genotypes, the exotic one with sowing in 1 November gave the highest seed yield (4.54 g·plant-1;3.00 t·ha-1).
文摘Essential oils from newly produced (2017) and previous season crop (2016) of cumin (Cuminum cyminum L.) seeds collected from local farmers in Dongla area, north Sudan were extracted and analyzed to determine the effect of storage period on volatile oil yield and quality. The essential oil was extracted using hydro distillation method. Identification of the volatile compounds was performed using gas chromatography-mass spectrometry (GC/MS). Results revealed that oil obtained from cumin (Cuminum cyminum L.) was pale yellow. With respect to oil characteristics, the specific gravity, refractive index, acid value, ester value and other quality characters were not significantly different between the stored and newly harvested seeds. Thirty-eight compounds were separated and identified in cumin seeds essential oil in both seasons with some minor differences in some components between 2016 and 2017 samples. Major effective components of cumin seeds volatile oil were cumin alcohol (4-isopropylbenzyl alcohol), cumin aldehydes as 4-Isopropylbenzaldehyd and 7,7-dimethylbicyclo (4.1.0) hept-3-ene-4-carbaldehyde known as carenal, terpinenes as γ-terpinene and α-terpinene (1,4-Cyclohexadiene, 1-methyl-4-(1-methylethyl) and 1,3-cyclohexadine-1-methanol, 4(1-methethyle)-), β-pinene, o-cymene and p-menthadienol in both stored and newly harvested samples. Differences between the samples season wise were very minor with respect to type of volatile components but percentage oil yield was largely decreased by storage period.
文摘Black cumin (<em>Nigella sativa</em> L.) the highly aggregate valuable medicinal plant was field cultivated for two subsequent seasons (2018, 2019) designed as factorial split plot based on randomized complete block with 3 replications. The main factors 4 elicitors: salicylic acid, (SA) Nano-selenium (NPs), yeast (YS) chitosan (CH) and (E0), control. Whereas, the sub-main plot 4 biofertilizers, dray Moringa leaves extract, (MLE), neem dray leaves extract (NME), humic acid (HA) and traditional (NPK) chemical fertilizer as control. Allied statistical analysis of variance revealed that biotic and abiotic elicitors coincide biofertilizer and NPK chemical fertilizer actuated significant positive impacts, dray seed, seed fixed oil, seed essential oil yield production. Also, significantly amelioration bioactive major fatty acids content of seed fixed oil (linolenic > carvone) dihydrolenoleic > oleic) as well as major terpens content of seed essential oil (P-cymene > thnymoquione > Penine). Consequently, multi-repeating elicitation cod be considered reliable strategy achieve sustainable development for <em>N-sativa</em> under, biotic elicitor coincide biofertilizers that excel abiotic elicitors coincide biofertilizer which excel biotic or abiotic elicitors coincide NPK traditional chemical fertilizer.
基金Supported by Natural Science Found of Zhejiang Province:Research of Molecular Mechanism of Curcumin Reversing TKI Targeted Drug Resistance of NSCLC(No.LY13H160037)
文摘OBJECTIVE: To examine whether a combinative treatment with curcumin enhances the effects of the epidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI) gefitinib on cell proliferation, clonogenic capacity and apoptosis in the drug-resistant lung cancer cell line NCI-H1975, and further investigate the molecular mechanisms involved.METHODS: NCI-H1975 cells were treated with curcumin and gefitinib alone or in combination, and cell proliferation, clonogenic capacity and apoptosis were examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, clone forming experiments, and flow cytometry, respectively, while p38, extracellular regulated protein kinase(ERK)1/2, and protein kinase B(AKT)phosphorylation were examined using Western blotting.RESULTS: Compared with the effects of either agent alone, the combination of curcumin and gefitinib had a stronger suppressive effect on proliferation and the clonogenic capacity(P < 0.05), and showed an increased ability to promote apoptosis(P < 0.05) and reduce p38, ERK1/2, and AKT phosphorylation(P < 0.05).CONCLUSION: Co-treatment of curcumin and gefitinib significantly improves the ability of gefitinib to inhibit cell proliferation, suppress the clonogenic capacity and enhance apoptosis in NCI-H1975 cells,and these effects are possibly mediated via a decrease in phosphorylation of proteins in downstream pathways of the epidermal growth factor receptor.
