Exploration of Construction Organization for Maintenance of Steam Turbine Equipment in Thermal Power Plants

The continuous development of the power industry has had a positive impact on thermal power plants,helping them maintain a good production form.In the use of steam turbine equipment in thermal power plants,to prolong its lifespan and avoid safety hazards,it is necessary to pay attention to strengthening maintenance and construction organization,better implementing effective organizational work,and effectively applying steam turbine equipment to ensure the sustainable development of thermal power plants.This article discusses the concept of equipment maintenance from the perspective of steam turbine equipment in thermal power plants,analyzes the current situation of equipment maintenance,and proposes a specific construction organization to provide a reference for steam turbine equipment maintenance.

Research on Construction Mode and Plant Selection of Linpan in Chengdu Plain

On the basis of investigation and analysis of Linpan in Chengdu Plain,in view of the ongoing reconstruction engineering of residential styles in Chengdu Plain,construction mode of Linpan in Chengdu Plain at new period had been proposed and plants had been selected in accordance with the function features of different modes. The initial study result was of certain instructive significance to the protection and development of Linpan in Chengdu Plain to some extent.

Construction of Plant Landscape at Cold Region——Plants Cultivation Design in South Lake Park of Hongqi Mountain in China

The paper had pointed out that constructing plant landscape of regional characteristic and developing and utilizing plant landscape resources to the maximum were top priorities of landscape design in North China.Based on the research on characteristics of plant landscape in North China,it concluded that plants at North China had short viewing time,limited species,and distinct seasonable landscape.By taking plant cultivation design in South Lake Park at Hongqi Mountain on the east of Heilongjiang Province for example,application of all plant landscaping means in landscape design had been analyzed.By combining with current status of South Lake Park on Hongqi Mountain,corresponding design principles and features had been identified,and better approaches had been provided for construction of plant landscape in North China through reasonably arranging landscape zones,skillfully dividing spatial types,meticulously selecting plant species,and diversifying plant shapes.It had divided 8 plant landscape zones and 12 theme plant landscapes in the park,creating plant landscape full of regional feature and cultural connotation.It hoped to provide reference for creation of different plant landscapes in North China.

Construction of Plant Expression Vector for Recombinant Human Epidermal Growth Factor (hEGF)

ObjectiveThis study aimed to construct plant expression vector for recombinant human epidermal growth factor （hEGF） and further to provide a basis for the expression of hEGF in peanut hairy root system. MethodAccording to the hEGF sequence in GenBank, hEGF was synthesized artificially; subsequently, hEGF gene was ligated with green fluorescent protein （GFP） gene, and their ligation product was then amplified with primers flanked with corresponding endonuclease cleavage sites, followed by double digestion by Sal I and EcoR I of the amplified products; next, pRI 101 AN DNA was extracted and digested by both Sal I and EcoR I; susequently, the digestion products of hEGF and GFP ligation fragment by Sal I and EcoR I and the digestion products of pRI 101 AN plasmid DNA by Sal I and EcoR I were ligated, and their ligation product was transformed into Escherichia coli XL10-Gold, followed by extraction of DNA from the recombinants exhibiting green fluorescence, which was then identified by enzymatic digestion and PCR, and the verified recombinant plasmid DNA was named pBZG101. ResultHuman epidermal growth factor gene （hEGF） and green fluorescent protein gene （GFP） were successfully ligated, and their ligation fragment was successfully ligated to pRI 101 AN DNA, finally with the acquirement of the plant expression vector for recombinant human epidermal growth factor-（pBZG101）. ConclusionThe plant expression vector for recombinant human epidermal growth factor-（pBZG101）- was successfully constructed in this study.

Application of Aromatic Plants in Landscape Construction of Hainan Island

Significance of aromatic plants for the landscape construction on Hainan Island was analyzed from 3 perspectives:urgent needs of urban construction,therapeutic effects of plants,and economical efficiency,and 3 major characteristics of aromatic plants were summarized as:ornamental effects,aromatic properties and health care capacity.Application forms and ranges of aromatic plants in landscape design were introduced,and application principles were given as below:A."right species for right land",paying attention to the consistency between biological characteristics and environment;B.focusing on functions of plants,highlighting the harmony between plant landscapes and environment;C.in a relative high density,highlighting landscape layer of aromatic plants;D.proper combinations,highlighting primary fragrances.All of the above can be summarized as "right species for right land,focusing on plant functions,proper combinations,and targeted on landscape effects".The authors proposed that design modes of aromatic plant landscapes fit for Hailan Island should be refined to apply the theories of constructing aromatic city into the detailed planning.

Analysis of Textbook "Garden Plant Protection" Construction in Agricultural and Forestry Universities or Higher Vocational Colleges

The survey of textbook 'Garden Plant Protection' was introduced in this study,and the necessity of textbook 'Garden Plant Protection' construction in agricultural and forestry universities or higher vocational colleges was also analyzed.Aimed at the status of textbook 'Garden Plant Protection' construction in agricultural and forestry universities or higher vocational colleges,the corresponding ideas for its construction were put forward.

Construction of Plant Virus Expression Vector pClYVV/CP/W and Expression of GFP

[ Objective] The study was to report the construction of plant virus expression vector pCIYVV/CP/W and the expression of green fluorescent protein（GFP） with pCIYVV/CP/W, and to develop effective plat virus vector for plant bioreactor to produce useful protein. [ Method] A section of multiple cloning sites among NIb/CP genes in pCIYVV genome and deoxyribonucleotide polylinker of cleavage recognition sequence containing viral protease Nla were cloned with infectivity full-length cDNA of clover yellow vein virus （CIYVV）, and pCIYVV/CP/W vector was constructed, GFP gene was inserted into pCIyVV/CP/W to construct the pCIYVV/CP/W/GFP vector. The transcription situation of recombinant virus clone was detected by RT-PCR, and targeted gene products expressed by recombinant virus clone were detected with western blot （WB）. [Result] The broad bean seedling inoculated with pCIYVV/CP/W/GFP expressed the same symptom as wild type CIYVV, morbidity was of 100%, the result showed that recombinant virus clone pCIYVV/CP/W/GFP didn＇t suppress, insertion of foreign gene didn＇t destroy the open reading frame of pCIYVV/CP/W. Foreign gene can keep living in F, progeny virus genorne steadily, recombinant virus clone pCIYVV/CP/W/GFP could steadily express GFP in progeny virus at least.[ Conclusion] The useful plant virus vector was provided for useful protein expressing.

On the Construction of Plant Landscape Form in Urban Landscape Architecture

Design of plant landscape form is a critical part of plant landscaping, and it is an important direction of modern researches on urban plant landscaping to promote the development of plant landscape via the interaction between landscape form design and other means. By elaborating five major influence factors of landscape plants, namely "flexibility", "art", "subject", "mode", and "artistic conception", this paper explored the application of construction thinking in the construction of urban plant landscape form, in order to provide theoretical basis for the development of urban plant landscape form.

Evaluation of Animal and Plant Resources Status Quo after the Reservoir Construction in Turks River and Protection Measures

[Objective] The aim was to assess the status quo of animal and plant resources in Turks River after the construction of the reservoir.[Method] Through field investigation,document check and sample identification,the distribution of animal and plants resources in Turks River after the construction of the reservoir was studied and corresponding protection measures were proposed.[Result] Under the influence of reservoir,there were fifteen types of rare animals,one species of national primary protected animals,seven species of national secondary protected animals,one species of provincial primary protected animals,six species of provincial secondary protected animals;there were twenty one species of rare plants,including two species of national secondary plant,five species of national tertiary protected animals,six types of Xinjiang primary protected plants,two species of Xinjiang secondary protected plants and six types of tertiary protected plants in Xinjiang.Protective policies for the land ecological environment were proposed,including corresponding compensation according to the rules and policies of government,plans being formulated for rare species protection,construction of natural reserves and artificial breeding base,usage of the environment in the reservoir and changes of ecological situation etc.[Conclusion] The study provided basis for the protection of animal and plants resources in Turks.

Cloning of a flower-specific expression promoter from Arabidopsis thaliana and its plant expression vector construction

Given the sequence of Chs gene promoter from Arabidopsis thaliana reported in GenBank （AF248988）, a pair of specific PCR primers was designed with the Primer Premier 5.0 software. PCR products of about 0.5 kb were successfully amplified with the genome DNA of A. thaliana as a DNA template and Taq polymerase as DNA polymerase. The purified PCR products were ligated to the pMD18-T vector. The sequencing result showed that the Chs promoter from A. thaliana was 531 bp long. Sequence alignment analysis based on the DNAMAN software revealed that the sequence similarity between the cloned promoter and target promoter （AF248988） was up to 100%. Online PLACE analysis indicated that the Chs promoter contained cis-elements such as TATA-box, CAAT-box, pollen-box, G-box, ACGT-containing element, R response element, Myb recognition element and TACPyAT-box. At the same time, a plant expression vectorpAtChs：：GUS which fused the Chs promoter and the marker gene GUS was successfully constructed.

Discussion on the Problems and Developing Trend of Construction of Sewage Treatment Plants in Small Towns

Existing problems of sewage treatment plants in small towns were summarized firstly,and then the developing trend of construction of sewage treatment plants in small towns was discussed from the aspects of project decision,site selection,design,processing technology and construction financing models. Finally,outlooks for its future development were proposed to provide reference for the construction of sewage treatment plants in small towns in future.

Research on the Construction of Remote Plant and Animal Hospital in Omnimedia Era

According to characteristics of information communication and sevices in omnimedia era, this paper studied the plan of constructing remote plant end animal hospital, which provided remote diagnosis service by expert team of doctors based on the co-building and sharing medical case resource bases through the reasonable management model on cloud services platform, and discussed the model for service and commerce of remote plant and animal hospital.

STUDIES ON STEROIDAL PLANT GROWTH REGULATOR 24. STEREOCONTROLLED CONSTRUCTION OF THE SIDE CHAIN OF BRASSINOLIDE AND HOMOBRASSINOLIDE VIA A TANDEM VICINAL DIALKYLATION OF PYRANONE MOIETY

A stereocontrolled construction of the side chain of brassinclide and homobrassinolide has been achieved via the tandem vicinal dialkylation of the pyranone moiety as a key step.

Project Construction and Important Technical Innovation for Qinshan Phase Ⅲ (PHWR) Nuclear Power Plant

Qinshan Phase Ⅲ(PHWR)Nuclear Power Plant,the first commercial heavy water reactor nuclear power plant in China,was the biggest trade project performed between the governments of China and Canada.As the owner,the Third Qinshan Nuclear Power Company(TQNPC)persisted in independent innovation management during the project construction,commissioning and self-dependent operation,efficiently realizing the three controls of the project,i.e.quality control,schedule control and investment control,and persisted in technical improvement on the basis of digestion and absorption of CANDU-6 technology to improve the unit safety and reliability.The project construction practice has helped China's nuclear power project management to becomeprogrammed,computerized,standardized and internationalized management from the existing basis.After completion of the project,with unit safe and steady operation as the prerequisite,TQNPC performed several technical modifications and innovations to continuously improve the unit performance.In the area of staff development,TQNPC paid much attention to cultivation of corporate culture,strengthed staff training and built up a good circulating mechanism with staff training and project construction promoting each other.Further to "Zero Breakthrough" and a new step forward of locolization successfully realized in Qinshan Nuclear Power Plant and Nuclear Power Qinshan Joint Venture Company,the improvement and developemnt of nuclear power project management level in Qinshan Phase Ⅲ(PHWR)Nuclear Power Plant provided reference for promotion of nuclear power development in China and standardized management of introducing large imported project.

Research on Key Techniques of Green Planting Construction in Landscaping Project

Due to the continuous development of urbanization,it had a serious impact on urban ecology.Therefore,techniques to improve the living environment and quality of life in urban areas as well as ways to improve aesthetics and greening of urban gardens have gradually become the focus of attention.Re-building and arranging the green plant community rationally can effectively improve the quality of people’s living environment.Besides,by maximizing ecological functions,it can better improve the quality of urban environmental.This paper introduces key technologies of green planting construction in urban landscaping projects and suggest ways to improve the urban ecological environment by viewing it from existing problems in the current urban ecological environment.

Construction of Plant Expression Vector for Hand,Foot and Mouth Virus EV71-VP1 Gene and Its Expression in Tomato

EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.