A new species of the gudgeon genus Huigobio Fang,1938(Cypriniformes:Cyprinidae)from the Yangtze River Basin,southern China

During revision of the genus Microphysogobio Mori,1934,we discovered a new species of Huigobio from the Yangtze River Basin in China.The new species,named Huigobio heterocheilus sp.nov.,can be distinguished from its congeners by a combination of the following characters:medial pad on lower lip not visible;ventral region scaleless before pelvic-fin insertion;lateral-line scales 39-40;caudal peduncle depth 49.2%-59.1%of length;barbel length 6.6%-10.7%of head length;black blotch between anterior margin of eye and upper lip.Using the K2P model and cytochrome b(cyt b)sequences,interspecific genetic distances between the new species and Huigobio exilicauda Jiang&Zhang,2013 and Huigobio chenhsienensis Fang,1938 were 13.2%and 15.1%,respectively.The new species is known from the upper reaches of the Xiangjiang River in the middle Yangtze River Basin.A diagnostic key for species of Huigobio Fang,1938 is provided.

The new discovery of Oligocene cyprinidae fossil in Qaidam basin, Northwest China

1 Introduction Some Cyprinidae fossils have been reported about 420km east of the fossil site in the same period of Wulan Husentu1 layer,and have been used as an important fossil evidence for a deep understanding of the

Study on DNA Barcoding of Four Common Cyprinidae Species

This study aimed to analyze the partial sequence of COI gene in Pengze crucian carp,gold crucian carp,Ukraine carp and common carp,with silver carp and tilapia fish as two outgroups.DNA fragments 814 bp in length were amplified and analyzed.Statistical analysis of sequence compositions and analysis of intraspecific and interspecific genetic distances were performed by using Mega4.1 software.The phylogenetic tree of COI gene was constructed with neighbor-joining method.According to the results,15 fish individuals could be divided into five groups,which was consistent with the results of traditional taxonomy.Furthermore,the partial sequence of COI gene was a good DNA barcode to identify different species and exhibited certain applicability in the analysis of interspecific phylogenetic relationships of Cyprinidae species.

Molecular phylogenetic relationships of Eastern Asian Cyprinidae(Pisces:Cypriniformes)inferred from cytochrome b sequences

Complete mitochondrial cytochrome b sequences of 54 species, including 18 newly sequenced, were analyzed to infer the phylogenetic relationships within the family Cyprinidae in East Asia. Phylogenetic trees were generated using various tree-building methods, including Neighbor-joining (NJ), Maximum Parsimony (MP) and Maximum Likelihood (ML) methods, with Myxocyprinus asiaticus (family Catostomidae) as the designated outgroup. The results from NJ and ML methods were mostly similar, supporting some existing subfamilies within Cyprinidae as monophyletic, such as Cultrinae, Xenocyprinae and Gobioninae (including Gobiobotinae). How-ever, genera within the subfamily 揇anioninae?did not form a monophyletic group. The subfamily Leuciscinae was divided into two unrelated groups: the Leuciscinae in East Asia forming as a monophyletic group together with Cultrinae and Xenocyprinae, while the Leuciscinae in Europe, Siberia, and North America as another monophyletic group. The monophyly of subfamily Cy-prininae sensu Howes was supported by NJ and ML trees and is basal in the tree. The position of Acheilognathinae, a widely accepted monophyletic group represented by Rhodeus sericeus, was not resolved.

Phylogenetic relationships of Cyprinidae (Teleostei:Cypriniformes) inferred from the partial S6K1 gene sequences and implication of indel sites in intron 1

The family Cyprinidae is widely distributed in East Asia,; has the important phylogenetic significance in the fish evolution. In this study, the 5′ end partial sequences (containing exon 1, exon 2; indel 1) of S6K1 gene were obtained from 30 representative species in Cyprinidae; outgroup using PCR amplification; sequencing. The phylogenetic relationships of Cyprinidae were reconstructed with neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML),; Bayesian methods. Myxocyprinus asiaticus (Catostomidae) was assigned to the outgroup taxon. Similar phylogenetic relationships within the family Cyprinidae were achieved with the four analyses. Leuciscini; Barbini were monophyletic lineages respectively with the high nodal supports. Leuciscini comprises Hypophthalmichthyinae, Xenocyprinae, Cultrinae, Gobioninae, Acheilognathinae; East Asian species of Leuciscinae; Danioninae. Monophyly of East Asian clade was supported with high nodal support. Barbini comprises Schizothoracinae, Barbinae, Cyprininae; Labeoninae. The monophyletic lineage consisting of Danio rerio, D. myersi,; Rasbora trilineata was basal in the tree. In addition, the large fragment indels in intron 1 were analyzed to improve the understanding of Cyprinidae relationships. The results showed that the large fragment indels were correlated with the relations among species. Some conserved regions in intron 1 were thought to be involved in the functional regulation. However, no correlation was found between sequence variations; species characteristic size.

Cyprinid phylogeny based on Bayesian and maximum likelihood analyses of partitioned data:implications for Cyprinidae systematics

Cyprinidae is the biggest family of freshwater fish, but the phylogenetic relationships among its higher-level taxa are not yet fully resolved. In this study, we used the nuclear recombination activating gene 2 and the mitochondrial 16S ribosomal RNA and cytochrome b genes to reconstruct cyprinid phylogeny. Our aims were to （i） demonstrate the effects of partitioned phylo- genetic analyses on phylogeny reconstruction of cyprinid fishes; （ii） provide new insights into the phylogeny of cyprinids. Our study indicated that unpartitioned strategy was optimal for our analyses; partitioned analyses did not provide better-resolved or -supported estimates of cyprinid phylogeny. Bayesian analyses support the following relationships among the major mono- phyletic groups within Cyprinidae： （Cyprininae, Labeoninae）, （（Acheilognathinae, （（Leuciscinae, Tincinae）, Gobioninae））, Xenocyprirlinae）. The placement of Danioninae was poorly resolved. Estimates of divergence dates within the family showed that radiation of the major cyprinid groups occurred during the Late Oligocene through the Late Miocene. Our phylogenetic analyses improved our understanding of the evolutionary history of this important fish family.

Acrossocheilus multistriatus, a new species of barbine fish(Teleostei: Cyprinidae) from the Zhujiang River basin, South China

A new barbine cyprinid fish, Acrossocheilus multistriatus sp. nov., is described from the Zhujiang River （Pearl River） basin in South China. It is separated from all other barred congeners except A. monticola by its unique coloration pattern of 8-12 vs. 5-7 vertical dark bars on the dorsum and flank. Although 8 vertical black bars are also present in specimens ofA. monticola, the new species is distinct from it by having a well-developed （vs. poor-developed） lower lip, with or without a median interruption （vs. with a median interruption which wider than 1/2 mouth width） on lower jaw, the lower jaw entirely covered （vs. uncoverd） by the lower lip, body depth 22.57%-25.22% SL （vs. 25.64%-32.26% SL）. Additional characteristices as follows： last simple dorsal-fin ray soft without serration; dark stripes on membranes between dorsal-fin rays; dark stripes absent on membranes between anal fin rays; maxillary barbels longer than eye diameter, extending to posterior edge of eyes.

Molecular variation and evolution of the tyrosine kinase domains of insulin receptor IRa and IRb genes in Cyprinidae

The insulin receptor （IR） gene plays an important role in regulating cell growth, differentiation and development. In the present study, DNA sequences of insulin receptor genes, IRa and IRb, were amplified and sequenced from 37 representative species of the Cyprinidae and from five outgroup species from non-cyprinid Cypriniformes. Based on coding sequences （CDS） of tyro- sine kinase regions of IRa and IRb, molecular evolution and phylogenetic relationships were analyzed to better understand the characteristics of IR gene divergence in the family Cyprinidae. 1Ra and IRb were clustered into one lineage in the gene tree of the IR gene family, reconstructed using the unweighted pair group method with arithmetic mean （UPGMA）. IRa and IRb have evolved into distinct genes after IR gene duplication in Cyprinidae. For each gene, molecular evolution analyses showed that there was no significant difference among different groups in the reconstructed maximum parsimony （MP） tree of Cyprinidae; IRa and 1Rb have been subjected to similar evolutionary pressure among different lineages. Although the amino acid sequences of IRa and IRb tyrosine kinase regions were highly conserved, our analyses showed that there were clear sequence variations between the tyrosine kinase regions of IRa and IRb proteins. This indicates that IRa and IRb proteins might play different roles in the insulin signaling pathway.

Recurrent polyploidization within widely distributed goldfish(the Carassius auratus species complex,Cyprinidae,Cypriniformes) in Euroasia

Prof.Zhang Yaping’s laboratory at the School of Life Sciences,Yunnan University,and Kunming Institute of Zoology,Chinese Academy of Sciences,reported Biogeography and recurrent polyploidization on goldfish,which was published in J Biogeography(2012,39:2264—2278)and Heredity(2014,112:415—427).Goldfish of the Carassius auratus complex is of special interest,which lies in two aspects:its wide

湖南鱼类新纪录一种——湖北圆吻鲴

2013年11月—2015年3月,笔者在对沅江洞庭湖段进行鱼类资源调查过程中,采得标本2万余尾,其中1种为未报道的湖南鱼类新纪录——湖北圆吻鲴Distoechodon hupeinensis Yih,标本鉴定依据伍献文等（1964）、成庆泰等（1978）。标本现保存于湖南师范大学生命科学学院脊椎动物标本室。湖北圆吻鲴Distoechodon hupeinensis Yih,属鲤形目Cypriniformes鲤科Cyprinidae圆吻鲴属Distoechodon。标本7尾,全长138~170 mm,体长111~141 mm。背鳍条Ⅲ-8;胸鳍条ⅰ-15;腹鳍条ⅰ-8;臀鳍条Ⅲ-9;鳃耙45-47;下咽齿2行,4.6-6.4,齿面截形;侧线鳞59-63。

拉氏1龄鱼种培育技术研究

拉氏（Phoxinus lagowskii）又称为洛氏,俗称柳根子,隶属于鲤形目Cypriniformes,鲤科Cyprinidae、雅罗鱼亚科Leuciscinae、属[1]Phoxinus,主要分布于新疆北部、黑龙江及图们江流域。该鱼在野生环境中主要以水生植物、藻类和水生昆虫为食,其生存水温要求不高,既可生活在冷水水域,也可生活在温水水域[2]。

Occurrence of Middle Miocene Fossil Cyprinid Fish in the Northern Qaidam Basin and its Paleoenvironmental Implications

With a thick sequence of early Eocene to Pleistocene terrestrial records, the Qaidam Basin on the northern Tibetan Plateau provides an important sedimentary archive for understanding the paleoenvironmental evolution of the northeast Tibetan Plateau. In this study, specimens of fossil fish remains are collected from the late Middle Miocene（Serravallian, -12 Ma） of the middle member of the Shang Youshashan Formation, Dahonggou（DHG） section, in the northern Qaidam Basin. Based on a systematic study of these materials, the remains have assigned to Cyprinidae, with typical pharyngeal teeth and dorsal fin spines with serrations on the posterior edge. Our discovery improves understanding of the cyprinid fish distribution characteristics in the Qaidam Basin during the Cenozoic. Cooccurrences of terrestrial brackish ostracod species Cyprideis and long chain alkenonesin the layer indicate that the studied cyprinid fish lived in a generally large brackish to saline water body during the late middle Miocene（Serravallian）, when the climate of Qaidam Basin was still not sufficiently dry to form an extreme saline water lake.

Five newly recorded Cyprinid fish (Teleostei： Cypriniformes) in Myanmar

Freshwater fish from the Putao and Myitkyina areas were collected in three ichthyofaunal surveys of the Mall Hka River and tributaries in and around Khakaborazi National Park and Hponkanrazi Wildlife Sanctuary, Kachin State, from 2014-2016. Tor yingjiangensis Chen et Yang 2004, Tor qiaojiensis Wu et al. 1977, Garra qiaojiensis Wu et al. 1977, Garra bispinosa Zhang 2005, and Schizothorax oligolepis Huang 1985, originally described from the upper Irrawaddy （Ayeyarwaddy） River in China, are first reported herein as new records to Myanmar. Counts, measurements, descriptions, photographs, and distributions of the specimens of the five newly recorded species are provided.

Identification of Species Composition of Fish in the Zarivar Lake (Kurdistan Province of Iran)

Zarivar Lake is a small, freshwater body in the far west of Iran, situated in the north Kurdistan Province close to the city of Marivan, with an area of about 750 ha and average water depth of 4 - 5 meters. During a survey of Zarivar Lake from 2010 to 2011, some specimens were obtained by seasonal sampling in 5 stations. They were used for gill net sampling in the lake. Due to the results, 6 species from 2 families in Zarivar Lake were identified. Most of the fish species belonged to Cyprinidae family. Totally, 6 species (Cyprinus carpio, Ctenopharyngodon idella, Capoeta damascina, Chalcalburnus sp., Hypophthalmichthys molitrix and Gambosia affinis) were identified from Cyprinidae and Poeciliidae families.

包头及乌梁素海现有鱼类

经初步调查,包头现有鱼类19种;乌梁素海现有鱼类18种。分属于3目5科。绝大多数属于鲤形目,少数分属于鲶目和鲈形目。鲤形目 Cynriniformes一、鲤科 Cyprinidae鲤科鱼类现有14种,分属于6个亚科。

祁连山裸鲤人工繁育试验

祁连山裸鲤（Cymnocypris chilianensis）,鲤科（Cyprinidae）、裂腹鱼亚科（Schizothoracinae）、裸鲤属（Gymno-cypris）。体裸露,胸鳍基部上方、侧线之下有不规则的鳞片;肛门和臀鳍两侧各有发达的大鳞,体背部黄褐色或灰褐色,腹部浅黄色或灰白色,体侧有大型不规则的块状暗斑;各鳍均带浅红色。适应性强,对生活条件并没有严格的要求,较小的水塘和较浅的湖边都能生活。

3D landscape reorganization in response to feeding preferences adaptation in the youngest split Gymnocypris fish

Divergence in feeding preference is one of the main mechanisms that lead to the emergence of new species.However,little is known about the underlying genetic mechanism of feeding preference adaptation,especially in the three-dimensional(3D)chromatin organization regulation.Gymnocypris eckloni scoliostomus(GS)and G.eckloni eckloni(GE)are two sister species of G.eckloni(Cyprinidae:Schizothoracinae:Gymnocypris).

基于S6K1基因部分序列的鲤科鱼类系统发育关系及内含子1Indel位点分析

鲤科鱼类在东亚分布广泛且数量丰富,在物种演化上具有重要的系统发育地位.本研究基于S6K1基因5′端功能调控序列,通过PCR扩增、克隆和测序,共获得30种鱼类S6K1前端部分DNA序列(外显子1,外显子2及内含子1),对序列变异进行分析,并采用邻接法(NJ)、最大简约法(MP)、最大似然法(ML)和贝叶斯法(Bayesian)重建鲤科鱼类系统发育关系.亚口鱼科的胭脂鱼(Myxocyprinus asiaticus)作为外类群,通过4种方法所得系统发育分支图大致相同,均以较高的节点支持率支持雅罗鱼系和鲃系的划分,雅罗鱼系包括雅罗鱼亚科东亚类群(East Asian group in Leuciscinae)、鲢亚科(Hypophthalmichthyinae)、鲴亚科(Xenocyprinae)、鲌亚科(Cultrinae)、亚科(Gobioninae)、鳑鲏亚科(Acheilognathinae)和亚科(Danioninae)部分种类;系统分支图也以较高的节点支持率支持鲤科鱼类东亚类群形成单系群.鲃系包括裂腹鱼亚科(Schizothoracinae)、鲃亚科(Barbinae)、鲤亚科(Cyprininae)和野鲮亚科(Labeoninae);另外,亚科中的斑马鱼(Danio rerio),麦氏(Danio myersi)和三线波鱼(Rasbora trilineata)形成一个单系群,位于系统树基部.以内含子1中大片段的插入或缺失(Indel)对物种分化及亲缘关系进行分析,以帮助理解鲤科鱼类系统发育关系或争议物种之间的亲缘关系.结果显示,内含子1中大片段的插入或缺失与物种分化具有一定的相关性,对于理解系统发育关系具有补充作用;内含子1中有许多区域较为保守,可能与功能调控有关,但本研究中并未发现S6K1序列变异与鱼类物种特征尺寸之间具有对应关系.

无公害淇河鲫高效养殖技术

淇河鲫是河南省特有的名贵土著鱼类,凭借其独有的"双背"外形和营养美味而深受消费者青睐,走俏市场。河南省大力推广无公害淇河鲫高效养殖,旨在发挥特色水产优势,促进渔民增收,丰富"菜篮子",同时更好地对淇河鲫鱼资源进行保护和商业开发。1淇河鲫的生物学简介淇河鲫(Carassius auratus in Qihe river)属鲤形目(Cypriniformes)鲤科(Cyprinidae)鲤亚科(Cyprini-dae),

Sequence variations of the S7 ribosomal protein gene in primitive cyprinid fishes: Implication on phylogenetic analysis

Cyprinidae is the largest fish family in the world and contains about 210 genera and 2010 species. Appropriate DNA markers must be selected for the phylogenetic analyses of Cyprinidae. In present study, the 1st intron of the S7 ribosomal protein (r-protein) gene is first used to examine the relationships among cyprinid fishes. The length of the 1st intron obtained by PCR amplification ranges from 655 to 859 bp in the 16 cyprinid species investigated, and is 602 bp in Myxocyprinus asiaticus. Out of the alignment of 925 nucleotide sites obtained, the parsimony informative sites are 499 and occupy 54% of the total sites. The results indicate that the 1st intron sequences of the S7 r-protein gene in cyprinids are rich in informative sites and vary remarkably in sequence divergence from 2.3% between close species to 66.6% between distant species. The bootstrap values of the interior nodes in the NJ (neighbor-joining) and MP (most-parsimony) trees based on the present S7 r-protein gene data are higher than