Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 ...Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 gene-mediated interaction with virus effector molecule. In this study, we searched for the susceptible allele of the “R” gene in Cv. T9. Southern hybridization study confirmed presence of an allele in Cv. T9. However, transcripts of the CYR1 could not be detected either by RT-PCR or by Northern hybridization in Cv. T9 and also in other susceptible blackgram line. The allele was isolated, sequenced and referred as cyr1. In silico study revealed that cyr1 also encodes a CC-NBS-LRR protein like CYR1. However the CC domain of cyr1 is truncated by 128 amino acid residues indicating functional impairment with respect to the signal transduction after pathogen invasion. Comparative 3D structural modeling, hydrogen bonding and Van der Waals interaction studies revealed differences between CYR1 and cyr1. Lys519 and Thr490 present in the largest pockets of the CYR1 are the key interacting hotspots between CYR1 and MYMIV coat protein (CP). The weak Van der Waals interactions and intermolecular hydrogen bonding between cyr1 and CP confers less stability to the molecular recognition complex, unlike CYR1. Thus, the present investigation revealed Cv. T9 shows compatible interaction with MYMIV due to the truncation in the cyr1 sequence and consequent structural difference in the N-terminal of CC-domain.展开更多
CCN1/CYR61(cellular communication network factor 1/cysteine-rich protein 61)是CCN家族成员,是一种重要的分泌基质蛋白。它在胚胎发育过程中对心血管的发育起着至关重要的作用;同时,在生物体成年期,其对炎症反应、血管生成、损伤...CCN1/CYR61(cellular communication network factor 1/cysteine-rich protein 61)是CCN家族成员,是一种重要的分泌基质蛋白。它在胚胎发育过程中对心血管的发育起着至关重要的作用;同时,在生物体成年期,其对炎症反应、血管生成、损伤修复、骨形成及过度纤维化和肿瘤等相关生理、病理过程起着关键作用。本文对CCN1/CYR61在骨再生中的作用作了简要总结,包括其对骨髓间充质干细胞、软骨细胞、成骨细胞及破骨细胞等骨再生相关细胞的调控,以及骨生成过程中必不可少的炎症反应和血管生成等生物学过程。展开更多
文摘Blackgram, an important legume crop, faces the constraint of Mungbean yellow mosaic India virus (MYMIV)-stress resulting in severe crop penalty. MYMIV-resistant plants exhibit incompatible response via a cognate CYR1 gene-mediated interaction with virus effector molecule. In this study, we searched for the susceptible allele of the “R” gene in Cv. T9. Southern hybridization study confirmed presence of an allele in Cv. T9. However, transcripts of the CYR1 could not be detected either by RT-PCR or by Northern hybridization in Cv. T9 and also in other susceptible blackgram line. The allele was isolated, sequenced and referred as cyr1. In silico study revealed that cyr1 also encodes a CC-NBS-LRR protein like CYR1. However the CC domain of cyr1 is truncated by 128 amino acid residues indicating functional impairment with respect to the signal transduction after pathogen invasion. Comparative 3D structural modeling, hydrogen bonding and Van der Waals interaction studies revealed differences between CYR1 and cyr1. Lys519 and Thr490 present in the largest pockets of the CYR1 are the key interacting hotspots between CYR1 and MYMIV coat protein (CP). The weak Van der Waals interactions and intermolecular hydrogen bonding between cyr1 and CP confers less stability to the molecular recognition complex, unlike CYR1. Thus, the present investigation revealed Cv. T9 shows compatible interaction with MYMIV due to the truncation in the cyr1 sequence and consequent structural difference in the N-terminal of CC-domain.
文摘CCN1/CYR61(cellular communication network factor 1/cysteine-rich protein 61)是CCN家族成员,是一种重要的分泌基质蛋白。它在胚胎发育过程中对心血管的发育起着至关重要的作用;同时,在生物体成年期,其对炎症反应、血管生成、损伤修复、骨形成及过度纤维化和肿瘤等相关生理、病理过程起着关键作用。本文对CCN1/CYR61在骨再生中的作用作了简要总结,包括其对骨髓间充质干细胞、软骨细胞、成骨细胞及破骨细胞等骨再生相关细胞的调控,以及骨生成过程中必不可少的炎症反应和血管生成等生物学过程。
