Two new C21 steroidal glycosides, cynanauriculoside I and cynanauriculoside II, were isolated from the roots of Cynanchum aurichulatum. Their structures were established using spectroscopic methods including one and ...Two new C21 steroidal glycosides, cynanauriculoside I and cynanauriculoside II, were isolated from the roots of Cynanchum aurichulatum. Their structures were established using spectroscopic methods including one and two-dimensional NMR.展开更多
Besides a series of normal C_(21) steroids from Marsdenia incisa incisa P.T.Li et Y.P.Li, it small amount of novel C_(21) steroidal glycoside (Ⅱ) had been isolated. The mild acidic hydrolysis of (Ⅱ) afforded genin-(...Besides a series of normal C_(21) steroids from Marsdenia incisa incisa P.T.Li et Y.P.Li, it small amount of novel C_(21) steroidal glycoside (Ⅱ) had been isolated. The mild acidic hydrolysis of (Ⅱ) afforded genin-(Ⅰ). By spectroscopic analyses of IR, ELMS, FDMS, ~1H NMR, ^(13)C NMR (DEPT, PRFT), ~1H-~1H COSY, ~1H-^(13)C COSY for (Ⅰ) and (Ⅱ), (Ⅰ) had been deduced as B-nor (7) -6β-formyl-pregnane-3β, 5β, 8β, 12β, 14β, 17β, 20-heptatol, named neomarinogenin; (Ⅱ) had been deduced as neomarinogenin 3-O-β-D-thevetopyranosyl-(1→-4)-β-Dcymaropyranosyl-(1→4)-β-D-cymaropyranoside, named neomarinoside. Their physical and spectral data were as follows. Neomarinogenin (Ⅰ). A colorless prism from Me_2 CO, mp 202—205℃, 〔α〕_D^(17)+28.81°(MeOH, c0.05), C_(21)H_(34)O_8 (Found: C, 60.76; H, 8.19, C_(21)H_(34)O_8, requires C, 60.87. H, 8.21％); IRv_(max)^(KBr)cm^(-1): 3450 (OH), 1700 (C=O), 1050(C-O-C); EIMS m/z: 397 (M-OH), 380 (397-OH), 379, 378 (M-2H_2O), 36; (M-3H_2O), 342 (M-4H_2O), 333,展开更多
A new C21 steroidal glycoside, named cynanversicoside F （1）, was isolated from the root of Cynanchum versicolor Bunge. Its structure was established as glaucogenin-A 3-O-β-D- digitoxopyranosyl-（1→4）-β-D-cymarop...A new C21 steroidal glycoside, named cynanversicoside F （1）, was isolated from the root of Cynanchum versicolor Bunge. Its structure was established as glaucogenin-A 3-O-β-D- digitoxopyranosyl-（1→4）-β-D-cymaropyranoside by spectroscopic and chemical methods.展开更多
The present study was designed to further investigate the C_(21) steroidal glycosides in Cynanchum plants.Two new steroidal glycosides based on a 13,14:14,15-disecopregnane-type aglycone,komaroside P(1) and komaroside...The present study was designed to further investigate the C_(21) steroidal glycosides in Cynanchum plants.Two new steroidal glycosides based on a 13,14:14,15-disecopregnane-type aglycone,komaroside P(1) and komaroside Q(2),together with three known compounds(3-5) were isolated from the whole herbs of Cynanchum komarovii.The aglycones of compounds 1 and 2 were two new disecopregnane.Their structures were elucidated on the basis of 1 D,2 D NMR spectroscopic data and acid hydrolysis.All the compounds(1-5) showed potent inhibitory activities against human leukemia cell lines(HL-60) with IC50 values ranging from 16.6 to 26.3 μmol·L^(-1),compared to the positive control 5-fluorouracil(6.4 μmol·L^(-1)).展开更多
文摘Two new C21 steroidal glycosides, cynanauriculoside I and cynanauriculoside II, were isolated from the roots of Cynanchum aurichulatum. Their structures were established using spectroscopic methods including one and two-dimensional NMR.
基金This work was supported by grants from International Foundation for Science in Sweden and National Science Foundation of China.
文摘Besides a series of normal C_(21) steroids from Marsdenia incisa incisa P.T.Li et Y.P.Li, it small amount of novel C_(21) steroidal glycoside (Ⅱ) had been isolated. The mild acidic hydrolysis of (Ⅱ) afforded genin-(Ⅰ). By spectroscopic analyses of IR, ELMS, FDMS, ~1H NMR, ^(13)C NMR (DEPT, PRFT), ~1H-~1H COSY, ~1H-^(13)C COSY for (Ⅰ) and (Ⅱ), (Ⅰ) had been deduced as B-nor (7) -6β-formyl-pregnane-3β, 5β, 8β, 12β, 14β, 17β, 20-heptatol, named neomarinogenin; (Ⅱ) had been deduced as neomarinogenin 3-O-β-D-thevetopyranosyl-(1→-4)-β-Dcymaropyranosyl-(1→4)-β-D-cymaropyranoside, named neomarinoside. Their physical and spectral data were as follows. Neomarinogenin (Ⅰ). A colorless prism from Me_2 CO, mp 202—205℃, 〔α〕_D^(17)+28.81°(MeOH, c0.05), C_(21)H_(34)O_8 (Found: C, 60.76; H, 8.19, C_(21)H_(34)O_8, requires C, 60.87. H, 8.21％); IRv_(max)^(KBr)cm^(-1): 3450 (OH), 1700 (C=O), 1050(C-O-C); EIMS m/z: 397 (M-OH), 380 (397-OH), 379, 378 (M-2H_2O), 36; (M-3H_2O), 342 (M-4H_2O), 333,
文摘A new C21 steroidal glycoside, named cynanversicoside F （1）, was isolated from the root of Cynanchum versicolor Bunge. Its structure was established as glaucogenin-A 3-O-β-D- digitoxopyranosyl-（1→4）-β-D-cymaropyranoside by spectroscopic and chemical methods.
基金supported by the National Natural Science Foundation of China(No.31370375)
文摘The present study was designed to further investigate the C_(21) steroidal glycosides in Cynanchum plants.Two new steroidal glycosides based on a 13,14:14,15-disecopregnane-type aglycone,komaroside P(1) and komaroside Q(2),together with three known compounds(3-5) were isolated from the whole herbs of Cynanchum komarovii.The aglycones of compounds 1 and 2 were two new disecopregnane.Their structures were elucidated on the basis of 1 D,2 D NMR spectroscopic data and acid hydrolysis.All the compounds(1-5) showed potent inhibitory activities against human leukemia cell lines(HL-60) with IC50 values ranging from 16.6 to 26.3 μmol·L^(-1),compared to the positive control 5-fluorouracil(6.4 μmol·L^(-1)).
