Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory seizures.To develop in viv...Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory seizures.To develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause CMT2.Mutations located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)model.In addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive control.Of these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut allele.We found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in Drosophila.Flies heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal morphology.These Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.展开更多
[Objective] The research aimed to investigate the influences of each factor in the combination of Ca（ClO）2 and O3 on removing carbendazim residues from kumquat. [Method] By using Box-Behnken response surface methodo...[Objective] The research aimed to investigate the influences of each factor in the combination of Ca（ClO）2 and O3 on removing carbendazim residues from kumquat. [Method] By using Box-Behnken response surface methodology （RSM）, the effects of initial concentration of carbendazim, soaked time of Ca（ClO）2 solution, soaked time of ozone water and Ca（ClO）2 concentration on removing carbendazim residues from kumquat were studied. [Result] In the test range, the effects of each factor on removing carbendazim residues from kumquat were soaked time of ozone water, soaked time of Ca（ClO）2 solution, initial residual amount of carbendazim and concentration of Ca（ClO）2 solution in turn. By fitting the experimental result, the quadratic polynomial model of relationship between residual amount of carbendazim and the above each factor was obtained. The verified experimental result showed that the model had high precision. [Conclusion] The model could be used to guide using the appropriate soaked time of Ca（ClO）2 solution, soaked time of ozone water and Ca（ClO）2 concentration under the certain initial concentration and residue limits of carbendazim, which guaranteed obtaining the expected residue limits of carbendazim.展开更多
基金supported by the Natural Science Foundation of Fujian Province,No.2020J02027the National Natural Science Foundation of China,No.31970461the Foundation of NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital,No.2022-NHP-05(all to WC).
文摘Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory seizures.To develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause CMT2.Mutations located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)model.In addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive control.Of these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut allele.We found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in Drosophila.Flies heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal morphology.These Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.
基金Supported by International Scientific and Technological Cooperation Plan in Changzhou City(cz20100028)~~
文摘[Objective] The research aimed to investigate the influences of each factor in the combination of Ca（ClO）2 and O3 on removing carbendazim residues from kumquat. [Method] By using Box-Behnken response surface methodology （RSM）, the effects of initial concentration of carbendazim, soaked time of Ca（ClO）2 solution, soaked time of ozone water and Ca（ClO）2 concentration on removing carbendazim residues from kumquat were studied. [Result] In the test range, the effects of each factor on removing carbendazim residues from kumquat were soaked time of ozone water, soaked time of Ca（ClO）2 solution, initial residual amount of carbendazim and concentration of Ca（ClO）2 solution in turn. By fitting the experimental result, the quadratic polynomial model of relationship between residual amount of carbendazim and the above each factor was obtained. The verified experimental result showed that the model had high precision. [Conclusion] The model could be used to guide using the appropriate soaked time of Ca（ClO）2 solution, soaked time of ozone water and Ca（ClO）2 concentration under the certain initial concentration and residue limits of carbendazim, which guaranteed obtaining the expected residue limits of carbendazim.
