WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with ...WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with different doses of X rays were analyzed for the changes in signal molecules of the phospholipase C phosphatidylinositol biphosphate(PLC IP2) and G protein adenylate cyclase(AC) pathways. [WT5”BX]Results.[WT5”BZ]It was found that[Ca 2+ ] i increased in response to doses within 0 2 Gy which was most marked after 0 075 Gy and the increase was accentuated in the presence of Con A. The changes in CD3 and calcineurin(CN) expression of the thymocytes followed the same pattern as the alterations in [Ca 2+ ] i after LDR. The expression of α,β1 and β2 isoforms of protein kinase C(PKC) was all up regulated after 0 075 Gy with the increase in PKC β1 expression being most marked. The cAMP/cGMP ratio and PKA activity of the thymocytes was lowered after low dose radiation and increased after doses above 0 5 Gy in a dose dependent manner, thus giving rise to J shaped dose response curves. The Ca antagonist TMB 8 and cAMP stimulant cholera toxin suppressed the augmented thymocyte proliferation induced by LDR. [WT5”BX]Conclusion.[WT5”BZ]Data presented in the present paper suggest that activation of the PLC PIP2 signal pathway and suppression of the AC cAMP signal pathway are involved in the stimulation of the thymocytes following WBI with low dose X rays.展开更多
T-2毒素微胶囊是制备虾毒饵料研究该毒素在对虾食品链中传递规律的前提技术,从T-2毒素微胶囊毒饵料的性状、包埋率及对虾利用率3个方面对其进行评价。采用微胶囊技术,以复合蛋白制剂为壁材,20 ng T-2毒素和对虾饲料为混合芯材,采用均质...T-2毒素微胶囊是制备虾毒饵料研究该毒素在对虾食品链中传递规律的前提技术,从T-2毒素微胶囊毒饵料的性状、包埋率及对虾利用率3个方面对其进行评价。采用微胶囊技术,以复合蛋白制剂为壁材,20 ng T-2毒素和对虾饲料为混合芯材,采用均质和物理加热法使复合蛋白制剂包裹混合心材,再用恒温干燥法使其硬化,形成颗粒状对虾T-2毒素微胶囊毒饵料,建立了T-2毒素微胶囊毒饵料的制备工艺。采用LC-MS/MS技术检测T-2毒素,普通毒饵料、微胶囊毒饵料抽提水样中T-2的毒素含量分别为2.661、0.654 ng/mL,微胶囊毒饵料的包埋率达96.73%,其利用率是普通毒饵料的1.7倍。研究表明T-2毒素微胶囊毒饵料可以解决对虾口服难溶于水的T-2毒素的问题,并提高了T-2毒素的利用率,为研究T-2毒素对对虾的作用提供技术支撑。展开更多
基金This work was supported by a grant from NSFC (No.39570188)
文摘WT5”BZ] To study the molecular mechanism of the stimulatory effect of low dose radiation(LDR) on T cell activation. [WT5”BX]Methods.[WT5”BZ] Thymocytes from Kunming mice exposed to whole body irradiation(WBI) with different doses of X rays were analyzed for the changes in signal molecules of the phospholipase C phosphatidylinositol biphosphate(PLC IP2) and G protein adenylate cyclase(AC) pathways. [WT5”BX]Results.[WT5”BZ]It was found that[Ca 2+ ] i increased in response to doses within 0 2 Gy which was most marked after 0 075 Gy and the increase was accentuated in the presence of Con A. The changes in CD3 and calcineurin(CN) expression of the thymocytes followed the same pattern as the alterations in [Ca 2+ ] i after LDR. The expression of α,β1 and β2 isoforms of protein kinase C(PKC) was all up regulated after 0 075 Gy with the increase in PKC β1 expression being most marked. The cAMP/cGMP ratio and PKA activity of the thymocytes was lowered after low dose radiation and increased after doses above 0 5 Gy in a dose dependent manner, thus giving rise to J shaped dose response curves. The Ca antagonist TMB 8 and cAMP stimulant cholera toxin suppressed the augmented thymocyte proliferation induced by LDR. [WT5”BX]Conclusion.[WT5”BZ]Data presented in the present paper suggest that activation of the PLC PIP2 signal pathway and suppression of the AC cAMP signal pathway are involved in the stimulation of the thymocytes following WBI with low dose X rays.
文摘T-2毒素微胶囊是制备虾毒饵料研究该毒素在对虾食品链中传递规律的前提技术,从T-2毒素微胶囊毒饵料的性状、包埋率及对虾利用率3个方面对其进行评价。采用微胶囊技术,以复合蛋白制剂为壁材,20 ng T-2毒素和对虾饲料为混合芯材,采用均质和物理加热法使复合蛋白制剂包裹混合心材,再用恒温干燥法使其硬化,形成颗粒状对虾T-2毒素微胶囊毒饵料,建立了T-2毒素微胶囊毒饵料的制备工艺。采用LC-MS/MS技术检测T-2毒素,普通毒饵料、微胶囊毒饵料抽提水样中T-2的毒素含量分别为2.661、0.654 ng/mL,微胶囊毒饵料的包埋率达96.73%,其利用率是普通毒饵料的1.7倍。研究表明T-2毒素微胶囊毒饵料可以解决对虾口服难溶于水的T-2毒素的问题,并提高了T-2毒素的利用率,为研究T-2毒素对对虾的作用提供技术支撑。
