Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment ...Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.展开更多
The challenge of high temperatures in deep mining remains harmful to the health of workers and their production efficiency The addition of phase change materials (PCMs) to filling slurry and the use of the cold storag...The challenge of high temperatures in deep mining remains harmful to the health of workers and their production efficiency The addition of phase change materials (PCMs) to filling slurry and the use of the cold storage function of these materials to reduce downhole temperatures is an effective approach to alleviate the aforementioned problem.Paraffin–CaCl_(2)·6H_(2)O composite PCM was prepared in the laboratory.The composition,phase change latent heat,thermal conductivity,and cemented tailing backfill (CTB) compressive strength of the new material were studied.The heat transfer characteristics and endothermic effect of the PCM were simulated using Fluent software.The results showed the following:(1) The new paraffin–CaCl_(2)·6H_(2)O composite PCM improved the thermal conductivity of native paraffin while avoiding the water solubility of CaCl_(2)·6H_(2)O.(2) The calculation formula of the thermal conductivity of CaCl_(2)·6H_(2)O combined with paraffin was deduced,and the reasons were explained in principle.(3) The“enthalpy–mass scale model”was applied to calculate the phase change latent heat of nonreactive composite PCMs.(4)The addition of the paraffin–CaCl_(2)·6H_(2)O composite PCM reduced the CTB strength but increased its heat absorption capacity.This research can give a theoretical foundation for the use of heat storage backfill in green mines.展开更多
为了全面评价豌豆蛋白水解物(pea protein hydrolysates,PPH)及其超滤分离组分的体外及细胞抗氧化能力,以豌豆蛋白为原料,采用碱性蛋白酶和复合蛋白酶协同水解制备PPH,然后超滤分级得到PPH1(分子质量<3 kDa)、PPH2(分子质量3~5 kDa)...为了全面评价豌豆蛋白水解物(pea protein hydrolysates,PPH)及其超滤分离组分的体外及细胞抗氧化能力,以豌豆蛋白为原料,采用碱性蛋白酶和复合蛋白酶协同水解制备PPH,然后超滤分级得到PPH1(分子质量<3 kDa)、PPH2(分子质量3~5 kDa)、PPH3(分子质量>5 kDa)3个组分,使用不同的化学方法研究PPH和超滤分离组分的抗氧化活性,从中选择抗氧化能力最高的组分进一步考察其细胞内自由基清除能力并用H_(2)O_(2)应激的HepG2细胞评估其对氧化应激的保护作用。结果表明,各组分中PPH1具有最高的体外化学抗氧化活性,其氨基酸组成分析表明,抗氧化功能氨基酸占比高;在实验设定浓度下,PPH1能够显著抑制H_(2)O_(2)应激损伤HepG2细胞内活性氧的积累,对细胞应激损伤有明显的保护作用。研究认为,PPH及其超滤分离组分具有良好的体外化学及细胞抗氧化活性,对H_(2)O_(2)应激的HepG2细胞具有保护作用,能够为其作为一种天然源抗氧化剂提供依据。展开更多
基金supported by the National Natural Science Foundation of China(31972091)。
文摘Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.
基金financial support provided by the National Natural Science Foundation of China (No. 52174106)the Key Technology Research and Development Program (No. 2022YFC2905102)。
文摘The challenge of high temperatures in deep mining remains harmful to the health of workers and their production efficiency The addition of phase change materials (PCMs) to filling slurry and the use of the cold storage function of these materials to reduce downhole temperatures is an effective approach to alleviate the aforementioned problem.Paraffin–CaCl_(2)·6H_(2)O composite PCM was prepared in the laboratory.The composition,phase change latent heat,thermal conductivity,and cemented tailing backfill (CTB) compressive strength of the new material were studied.The heat transfer characteristics and endothermic effect of the PCM were simulated using Fluent software.The results showed the following:(1) The new paraffin–CaCl_(2)·6H_(2)O composite PCM improved the thermal conductivity of native paraffin while avoiding the water solubility of CaCl_(2)·6H_(2)O.(2) The calculation formula of the thermal conductivity of CaCl_(2)·6H_(2)O combined with paraffin was deduced,and the reasons were explained in principle.(3) The“enthalpy–mass scale model”was applied to calculate the phase change latent heat of nonreactive composite PCMs.(4)The addition of the paraffin–CaCl_(2)·6H_(2)O composite PCM reduced the CTB strength but increased its heat absorption capacity.This research can give a theoretical foundation for the use of heat storage backfill in green mines.
