钙调素是生物体中一种重要的调控物质 ,它广泛存在于各种原核、真核生物中 ,在不同的组织和细胞内 ,通过与不同的 Ca MBPs结合参与不同功能的调控。因此 ,特定组织和细胞中 Ca MBPs的定位 ,特别是生理、病理条件下对其分布变化的研究 ,...钙调素是生物体中一种重要的调控物质 ,它广泛存在于各种原核、真核生物中 ,在不同的组织和细胞内 ,通过与不同的 Ca MBPs结合参与不同功能的调控。因此 ,特定组织和细胞中 Ca MBPs的定位 ,特别是生理、病理条件下对其分布变化的研究 ,对于阐明 Ca M的调控机制及生物体的生理机能、发病机制具有重要意义。本文对近些年在这方面的研究方法和有关工作进行了简要概述。展开更多
Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be elimina...Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be eliminated by addition of CaM. At the same time, the phosphorylation can also be inhibited by EGTA or CaM antagonists , such as TFP (trifluoperazine) and W 7 (N (6 aminohexyl) 5 chloro 1 naphthalene sulfonamide). This result implies that (i) Ca 2+ and CaM most likely participate in and regulate plant photosynthesis; (ii) the kinase that catalyzes thylakoid membrane protein phosphorylation can be regulated by Ca 2+ and CaM. However, the further experiments indicate that BP 10 has no effect on dephosphorylation of thylakoid phosphoproteins .展开更多
文摘钙调素是生物体中一种重要的调控物质 ,它广泛存在于各种原核、真核生物中 ,在不同的组织和细胞内 ,通过与不同的 Ca MBPs结合参与不同功能的调控。因此 ,特定组织和细胞中 Ca MBPs的定位 ,特别是生理、病理条件下对其分布变化的研究 ,对于阐明 Ca M的调控机制及生物体的生理机能、发病机制具有重要意义。本文对近些年在这方面的研究方法和有关工作进行了简要概述。
文摘Light induced phosphorylation of endogenous thylakoid membrane protein can be inhibited markedly by a novel inhibitor CaMBP 10 which is discovered and isolated from plant. The inhibitory effect of BP 10 can be eliminated by addition of CaM. At the same time, the phosphorylation can also be inhibited by EGTA or CaM antagonists , such as TFP (trifluoperazine) and W 7 (N (6 aminohexyl) 5 chloro 1 naphthalene sulfonamide). This result implies that (i) Ca 2+ and CaM most likely participate in and regulate plant photosynthesis; (ii) the kinase that catalyzes thylakoid membrane protein phosphorylation can be regulated by Ca 2+ and CaM. However, the further experiments indicate that BP 10 has no effect on dephosphorylation of thylakoid phosphoproteins .
