Physiological and Biochemical Responses of Perennial Ryegrass Mixed Planting with Legumes under Heavy Metal Pollution

In artificially controlled pot experiments,perennial ryegrass was mixed with other leguminous plants(white clo-ver and alfalfa)and treated with lead,zinc and cadmium(337 mg·kg^(-1),648 mg·kg^(-1),and 9 mg·kg^(-1),respectively)to simulate compound pollution conditions.The results showed that the concentrations of heavy metals,trans-port factors,and bioconcentration factors in mixed planting of ryegrass decreased compared with those in mono-culture.Regardless of whether heavy metal pollution was introduced,mixed planting increased the aboveground and underground biomasses of ryegrass.The different mixed planting treatments had no significant impact on the chlorophyll concentration of ryegrass.The mowing time,mixed planting treatment,and heavy metal treatment had impacts on antioxidant and osmotic adjustment substances,and there were some interactions.The mixed planting treatment did not significantly affect glutathione concentration,cysteine concentration,or nonprotein thiol.Mixed planting generally increased the nitrogen and phosphorus concentrations of ryegrass while reducing the stoichiometric ratio of carbon,nitrogen,and phosphorus.These results suggest that the mixed planting of ryegrass with legumes promotes the growth of ryegrass in the presence of high concentrations of heavy metal pollution.However,it does not enhance the ability of ryegrass to remediate heavy metal pollution in the soil.

Arbuscular mycorrhizal fungi inoculation and exogenous indole-3-acetic acid application induce antioxidant defense response to alleviate cadmium toxicity in Broussonetia papyrifera

Cadmium(Cd)contamination in soil poses a huge threat to plants even at low concentrations;Broussonetia papyrifera has great potential in remediation of soil heavy metal contamination.However,whether exogenous indole-3-acetic acid(IAA)application and arbuscular mycorrhizal fungi(AMF)have synergistic effects on Cd tolerance of B.papyrifera remains unclear.To investigate the effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress,two experiments were conducted:the first to investigate the effect of AMF(Rhizophagus irregularis)inoculation on the tolerance of B.papyrifera to Cd stress and the second to investigate the combined effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress.Parameters including endogenous hormone concentration,antioxidant defense response,malondialdehyde(MDA)content,and gene expression related to antioxidant enzyme system and hormone were measured.The results indicated that AMF alleviated Cd toxicity of B.papyrifera by reducing MDA content and improving antioxidant enzyme activities and Cd absorption capacity.Furthermore,the combination of AMF inoculation and IAA application had a synergetic effect on the tolerance of B.papyrifera to Cd stress through upregulating BpAUX1 and BpAUX2,which might contribute to root growth and root xylem synthesis,and by upregulating BpSOD2 and BpPOD34 to enhance the antioxidant enzyme system.This work provides a new insight into the application of IAA in the remediation of soil Cd pollution by mycorrhizal plants.

Cadmium accumulation in different pakchoi cultivars and screening for pollution-safe cultivars

The selection and breeding of pollution-safe cultivars (PSCs) is a practicable and cost-effective approach to minimize the influx of heavy metal to the human food chain.In this study,both pot-culture and field experiments were conducted to identify and screen out cadmium pollution-safe cultivars (Cd-PSCs) from 50 pakchoi (Brassica rapa L.ssp.chinensis) cultivars for food safety.When treated with 1.0 or 2.5 mg/kg Cd,most of the pakchoi cultivars (>70%) showed greater or similar shoot biomass when compared with the control.This result indicates that pakchoi has a considerable tolerance to soil Cd stress.Cd concentrations in the shoot varied significantly (P<0.05) between cultivars:in two Cd treatments (1.0 and 2.5 mg/kg),the average values were 0.074 and 0.175 mg/kg fresh weight (FW),respectively.Cd concentrations in the shoots of 14 pakchoi cultivars were lower than 0.05 mg/kg FW.In pot-culture experiments,both enrichment factors (EFs) and translocation factors (TFs) of six pakchoi cultivars were lower than 1.0.The field studies further confirmed that the Hangzhouyoudonger,Aijiaoheiye 333,and Zaoshenghuajing cultivars are Cd-PSCs,and are therefore suitable for growth in low Cd-contaminated soils (≤1.2 mg/kg) without any risk to food safety.

Research progress and strategies for multifunctional rapeseed： A case study of China

financially supported by the Key R＆D Program of Jiangxi Province of China （20152ACF60010）;the Key Projects of Hunan Science and Technology Support Program of China （Application research of Guiye A male sterile line）;the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China（JC1315）;supported by Emmy Noether DFG grant MA 6473/1-1

Remediation Effects of Water Management and Lime Application on Seasonally-Fallowed Cd-Contaminated Paddy Fields

In the light of the national policy of fallow, this study was conducted to determine how the different water management and lime application would affect soil physical and chemical properties, rice yield and cadmium （Cd） content of rice in fallow season. The results showed that, compared with the arid fallow, the waterlogging fallow decreased the soil pH value whereas signifcantly increased the soil organic matter content and the cation exchange quantity, and reduced the soil effective cadmium content and the rice cadmium content whereas could increase the rice yield to a certain extent. In the fooded fallow or the dry fallow, the application of lime mainly depended on the alkali conditioning of lime and the antagonistic effect of Ca2＋, which could signifcantly reduce the cadmium content of rice, and its effect would increase linearly with the increase of lime dosage, whereas had no significant effect on soil organic matter content and cation exchange quantity. In order to establish a linear equation of lime dosage and related indexes under the condition of waterlogging fallow or dry fallow, calculations showed that each application of lime at 1 000 kg/hm2 or kg/hmss2 could improve soil pH value by 0.238 2 or 0.246 5units respectively, and reduce the effective Cd content to 0.007 5 mg/kg both in the arid fallow and the waterlogging fallow conditions. The lime theoretic application rate for the lowest Cd content of late rice in the arid fallow was 5 120 kg/hm2, and the minimum limit of the Cd content in rice was 0.124 2 mg/kg; and the lime theoretic application rate for the highest yield of late rice in the submerged water fallow was 4 636 kg/hm2, the minimum theoretic Cd content in rice is 0.100 7 mg/kg, and it could reduce the Cd content in rice under the condition of submerged fallow and decrease the dosage of lime.

Graphene oxide influences bacterial community and soil environments of Cd-polluted Haplic Cambisols in Northeast China

Graphene oxide(GO),a carbon nanomaterial that is widely used in the environment and other industries,may pose potential risks to ecosystems,especially the soil ecosystem.Some soils in Northeast China are frequently polluted with cadmium(Cd) metal.However,there is no study on the influence of GO on the Cd-contaminated soil microbial community and soil chemical properties.In this study,Cd(100 mg kg^(-1))-polluted soils were treated with different concentrations of GO(0,25,50,150,250,and 500 mg L^(-1),expressed as T1,T2,T3,T4,T5,and T6,respectively) for 40 days.The treatment without Cd pollution and GO served as the control(CK).Then,we investigated the influence of the GO concentrations on the bacterial community and chemical properties of Cd-polluted Haplic Cambisols,the zonal soil in Northeast China.After GO addition,the richness and diversity indexes of the bacterial community in Cd-contaminated Haplic Cambisols initially increased by 0.05-33.92% at 25 mg L^(-1),then decreased by0.07-2.37% at 50 mg L^(-1),and then increased by 0.01-24.37%within 500 mg L^(-1) again.The species and abundance of bacteria varied with GO concentration,and GO significantly increased bacterial growth at 25 and 250 mg L^(-1).GO treatments influenced the bacterial community structure,and the order of similarity of the bacterial community structure was as follows:T4=T5> T1=T6> T2> T3> CK.Proteobacteria and Acidobacteria were the dominant bacteria,accounting for 36.0% and 26.2%,respectively,of soil bacteria.Different GO treatments also significantly affected the metabolic function of bacteria and further influenced the diversity of the bacterial community structure by affecting several key soil chemical properties:soil pH,organic matter and available potassium,phosphorus,and cadmium.Our results provide a theoretical basis for scientific and comprehensive evaluation of the environmental impacts of GO on the zonal forest soils of Northeast China.

Research progress and mechanism of nanomaterials-mediated in-situ remediation of cadmium-contaminated soil:A critical review

Cadmium contamination of soil is a global issue and in-situ remediation technology as a promising mitigation strategy has attracted more and more attention.Many nanomaterials have been applied for the in-situ remediation of cadmium-contaminated soil due to their excellent properties of the nano-scale size effect.In this work,recent research progress of various nanomaterials,including carbon nanomaterials,metal-based nanomaterials and nano mineral materials,in the removal of cadmium and in-situ remediation of cadmiumcontaminated soil were systematically discussed.Additional emphases were particularly laid on both laboratory and field restoration effects.Moreover,the factors which can affect the stability of cadmium,main interaction mechanisms between nanomaterials and cadmium in the soil,and potential future research direction were also provided.Therefore,it is believed that this work will ultimately contribute to the myriad of environmental cleanup advances,and further improve human health and sustainable development.

Spatio-temporal variation prediction on Cd content in the rice grains from Northern Zhejiang Plain during 2014–2019 based on high-precision soil geochemical data

In recent years,Cadmium(Cd)pollution has been found in many soil geochemical surveys in Northern Zhejiang Plain,a crucial rice production area in East China,located in the lower Yangtze River.To more scientifically predict the effect of soil Cd on rice safety,data including 348 local rhizosphere soil-rice samples obtained in 2014 were used in this study.Meanwhile,we extracted 90% of random samples as variables based on soil Cd content(Cd_(soil)),soil organic matter(SOM),pH,and other indicators.In addition,a multivariate linear model for rice Cd content(Cd_(rice))prediction based on the indicators including the soil Cd content(Cd_(soil)),the soil organic matter(SOM),and the pH value.The remaining 10%of random samples were used for the significance test.Based on the 2014 soil Cd content(Cd_(soil14))and the 2019 soil Cd content(Cd_(soil19)),this study predicted Cd content in 2019 rice grains(Cd_(p-rice19)).The spatio-temporal variation of Cdrice was contrasted in the five years from 2014 to 2019,and the risk areas of rice safety production were analyzed using the Geographical Information System(GIS).The results indicated that compared with the actual Cd content in 2014 rice grains(Cdrice14),the proportion of Cd_(p-rice19),which exceeded the standard food level in China(GB2762-2017),increased dramatically.Moreover,the high-value areas of Cdrice distributed greatly coincidentally in these two years.By contrast,both Cdrice and Cdsoil show very different spatial scales.The dominant reason is the distribution of the local canal systems,indicating that economic activities and agricultural irrigation may aggravate the risk of soil Cd pollution,thus threatening safe rice production.

Research Progressing of Present Contamination of Cd in Soil and Restoration Method

Cadmium is one of the important contaminative heavy metals. Due to continuous increase of discharge of Cadmium in industrial and agriculture production, cadmium contamination of soil is more and more serious day by day, which seriously threatens ecological environment and human health. Treatment of cadmium becomes an urgent issue and research hot point. At present, remedy for cadmium pollution upon soil mainly are physical restoration, chemical restoration and biological restoration, also there are measurements by means of adding external source matter and selecting low-Cd-accumulation varieties so as to reduce cadmium harm upon agricultural products. This article introduces in detail the resource of cadmium in soil and the present condition of cadmium pollution in farmland and processing of domestic cadmium pollution research, processing stage of domestic and foreign research of restoration of cadmium polluted soil as well as various peculiarities of repair methods. Finally, the expectation of research is discussed.