Cadmium （Cd） accumulation and toxicity in rice plants were characterized and identified by using brittle culm 1 （bcl）, a fragile rice mutant and its wild type （Shuangkezao, an indica rice） as materials by hydrop...Cadmium （Cd） accumulation and toxicity in rice plants were characterized and identified by using brittle culm 1 （bcl）, a fragile rice mutant and its wild type （Shuangkezao, an indica rice） as materials by hydroponics. The low Cd level didn＇t obviously affect the growth parameters in both rice genotypes, but under high Cd levels （1.0 and 5.0 μmol/L）, the growth of both rice plants were substantially inhibited. Moreover, bcl tended to suffer more seriously from Cd toxicity than Shuangkezao. Cd accumulation in both rice plants increased with the increase of Cd levels. There was a significant difference in Cd accumulation between the two rice genotypes with constantly higher Cd concentration in bcl, which also accumulated more Cd at 0, 0.1, and 1.0 μmol/L Cd levels. The same case was found in the two rice plants grown on Cd-contaminated soil. This suggested that cell wall might play an important role in Cd accumulation in rice plants by the physiological mechanisms. The malondialdehyde （MDA） content, superoxide dismutase （SOD） and peroxidase （POD） activities in rice plants were affected differently under Cd treatments, and which implied that POD might play the main role in detoxifying active oxygen free radical. A significant difference in antioxidative system between the two rice genotypes was found with constantly higher MDA content, SOD and POD activities in bcl. In summary, bcl accumulated more Cd and appeared to be more sensitive to Cd stress compared with its wild type.展开更多
目的:探讨益气活血方对阿霉素所致心肌毒性保护作用的机制。方法:Wistar大鼠40只,随机分为A组(空白组)、B组(阿霉素组)、C组(阿霉素+中药低剂量组)、D组(阿霉素+中药高剂量组)。A组予生理盐水2 m L/只隔日灌胃,其余各组均予阿霉素(2.5 m...目的:探讨益气活血方对阿霉素所致心肌毒性保护作用的机制。方法:Wistar大鼠40只,随机分为A组(空白组)、B组(阿霉素组)、C组(阿霉素+中药低剂量组)、D组(阿霉素+中药高剂量组)。A组予生理盐水2 m L/只隔日灌胃,其余各组均予阿霉素(2.5 mg/kg)隔日腹腔注射,C组与D组同时给予益气活血方(10.5 g/kg;5.25 g/kg)隔日灌胃,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、乳酸脱氢酶(LDH)含量,并观察心肌组织病理学变化。结果:与A组比较,B组大鼠血清MDA、LDH含量明显增高(P<0.01),SOD、GSH活性明显降低(P<0.01);与B组比较,C组、D组大鼠MDA、LDH含量明显降低(P<0.01),SOD、GSH活性升高(P<0.05或P<0.01)。结论:益气活血方能有效清除氧自由基而发挥抗氧化作用,在保证阿霉素用药剂量前提下有效防治其对心肌的毒性损伤。展开更多
文摘Cadmium （Cd） accumulation and toxicity in rice plants were characterized and identified by using brittle culm 1 （bcl）, a fragile rice mutant and its wild type （Shuangkezao, an indica rice） as materials by hydroponics. The low Cd level didn＇t obviously affect the growth parameters in both rice genotypes, but under high Cd levels （1.0 and 5.0 μmol/L）, the growth of both rice plants were substantially inhibited. Moreover, bcl tended to suffer more seriously from Cd toxicity than Shuangkezao. Cd accumulation in both rice plants increased with the increase of Cd levels. There was a significant difference in Cd accumulation between the two rice genotypes with constantly higher Cd concentration in bcl, which also accumulated more Cd at 0, 0.1, and 1.0 μmol/L Cd levels. The same case was found in the two rice plants grown on Cd-contaminated soil. This suggested that cell wall might play an important role in Cd accumulation in rice plants by the physiological mechanisms. The malondialdehyde （MDA） content, superoxide dismutase （SOD） and peroxidase （POD） activities in rice plants were affected differently under Cd treatments, and which implied that POD might play the main role in detoxifying active oxygen free radical. A significant difference in antioxidative system between the two rice genotypes was found with constantly higher MDA content, SOD and POD activities in bcl. In summary, bcl accumulated more Cd and appeared to be more sensitive to Cd stress compared with its wild type.
