Association of smoking, alcohol and NSAIDs use with expression of cag A and cag T genes of Helicobacter pylori in salivary samples of asymptomatic subjects

Objective:To determine the association of smoking, alcohol and nonsteroidal anti-inflammatory drugs(NSAIDs) use with presence and virulence of Helicobacter pylori(H. pylori) infection in a representative sample of a random adult population of asymptomatic subjects.Methods:Non virulent 16S rRNA and virulent cag A and T genes from salivary samples of 854 asymptomatic subjects were determined using polymerase chain reaction. The presence and absence of virulent and non virulent infection was statistically compared with consumption of smoking, alcohol and NSAID s.Results:The prevalence of infection in male and female subjects was found to be 69.25% and 66.90%, respectively. The prevalence of infection in the population of asymptomaticsubjects with respect to consumption of alcohol was as follows: current(31.22%), former(52.20%) and never(43.58%). The prevalence of infection in the population of asymptomatic subjects withrespect to smoking of cigarettes was as follows: current(88.80%), former(57.14%) and never(33.33%).The prevalence of infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 82.75%and 21.16%, respectively. Virulence in male and femalesubjects was found to be 60.00%and 50.00%, respectively. The presence of virulent infection in the population of asymptomatic subjects with respect to consumption of alcohol was as follows:current(28.57%), former(40.15%) and never(50.00%). The prevalence of virulent infection in the population of asymptomatic subjects with respect to smoking of cigarettes was as follows: current(79.32%), former(75.00%) and never(50.00%). The prevalence of virulent infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 88.23% and 66.66%, respectively.Conclusions:It can be concluded that smoking and NSAIDs consumption are aggravating factors for virulence ofH. pylori and alcohol can inhibitH. pyloriin fection in asymptomatic subjects.

CAG方案清除T细胞急性淋巴细胞白血病细胞株A3细胞作用机制的研究

本研究主要探讨CAG方案清除T细胞性急性淋巴细胞白血病(T-ALL)细胞株A3细胞的作用机制及G-CSF/G-CSFR配体受体系统在清除过程中的作用。以单克隆抗体结合流式细胞术测定A3细胞表面G-CSFR表达率;以A3细胞为体外实验模型,分组加入不同浓度的G-CSF作用48小时后收集细胞,碘化丙锭细胞核染色法分析细胞周期变化;用Cell Counting Kit(CCK-8)检测不同浓度阿糖胞苷(Ara-C)和G-CSF组合作用48小时后对细胞生长抑制率的影响;用AnnexinV试剂盒结合流式细胞术检测Ara-C、阿克拉霉素(ACR)及G-CSF联合作用48小时后细胞凋亡水平。结果表明:T-ALL细胞株A3细胞表面高表达G-CSFR(94.2%);G-CSF作用48小时后S期A3细胞比例在一定浓度范围内随G-CSF浓度的增加而升高;CCK-8检测显示Ara-C+G-CSF组较单药Ara-C组抑制细胞生长更明显(Ara-C浓度10-5mmol/L和10-6mmol/L时p<0.05);凋亡检测显示Ara-C+ACR+G-CSF组细胞早期凋亡率高达38%,显著高于Ara-C+ACR组。结论:T-ALL细胞株A3细胞表面高表达G-CSFR;G-CSF/G-CSFR配体受体系统在CAG方案清除A3细胞的过程中与化疗药物具有协同作用,即通过动员G0期细胞进入细胞增殖周期使细胞对化疗药物的敏感性增强、导致细胞凋亡,从而清除细胞。诱导凋亡是CAG方案清除T-ALL细胞株A3细胞的机制之一。

半量CAG方案联合地西他滨治疗老年初诊急性髓系白血病患者的效果

目的:观察半量CAG方案联合地西他滨治疗老年初诊急性髓系白血病患者的效果。方法:选取2020年1月至2023年1月于该院就诊的108例老年初诊急性髓系白血病患者进行前瞻性研究,按随机数字表法将其分为对照组和观察组各54例。对照组予以地西他滨治疗,观察组在对照组基础上联合半量CAG方案治疗,比较两组临床缓解率、住院时间、治疗前后T细胞亚群指标水平和治疗期间不良反应发生率。结果:观察组临床缓解率为72.22%(39/54),高于对照组的40.74%(22/54),差异有统计学意义(P<0.05);观察组住院时间短于对照组,差异有统计学意义(P<0.05);治疗后,两组CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)水平均低于治疗前,且观察组低于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:半量CAG方案联合地西他滨治疗老年初诊急性髓系白血病患者可提高临床缓解率,缩短住院时间,降低T细胞亚群指标水平,效果优于单纯地西他滨治疗。

环黄芪醇联合IL-7和IL-15对人脐带血T细胞体外扩增的影响

目的探究环黄芪醇(cycloastragenol,CAG)联合细胞因子IL-7和IL-15对人脐带血T细胞体外扩增和CD8^+T细胞亚群中干细胞样中心记忆性T细胞(stem cell-like central memory T cells,TSCM)比例的影响,为基于T细胞的肿瘤细胞免疫治疗研究奠定实验基础。方法采集健康产妇脐带血,通过密度梯度离心法分离获得脐带血单个核细胞(umbilical cord blood mononuclear cells,UCBMC),利用CD3/CD28抗体偶联磁珠刺激活化后,采用不同浓度的CAG(0、0.04、0.2、1、5μmol/L)联合IL-7和IL-15连续培养,利用多色流式细胞术分别测定T细胞的增殖程度以及CD8^+T细胞中TSCM比例的变化情况。结果 0.2μmol/L CAG与IL-7和IL-15细胞因子联用组促T细胞扩增作用显著高于其他浓度CAG与细胞因子IL-7和IL-15联用组或单独使用细胞因子IL-7和IL-15组。同时,0.2μmol/L CAG联用IL-7和IL-15组还可以促进CD8^+T中CD197^+CD45RA+细胞表型的表达,即维持CD8^+T中高比例的TSCM亚群。结论 0.2μmol/L CAG联合IL-7和IL-15可以显著提高活化T细胞扩增能力,并能保持CD8^+T细胞中高比例的TSCM亚群。

CAG方案治疗复发或初次诱导缓解治疗失败的T细胞急性淋巴细胞白血病

目的初步探索CAG方案对复发或初次诱导缓解治疗失败的T细胞急性淋巴细胞白血病(T-ALL)的疗效。方法6例复发或初次诱导缓解治疗失败的T-ALL患者接受CAG方案再次诱导缓解治疗,并分析该组患者的细胞形态学、免疫学、分子细胞遗传学及临床特征和治疗效果。结果6例患者经CAG方案再次诱导缓解治疗后均取得了完全缓解(CR),CR率100%;其具备T-ALL患者常见的临床特征如高外周血白细胞、髓外浸润常见等;细胞形态学均为ALL-L_1和L_2型;免疫分型均为不成熟T细胞表型;染色体多为正常核型;多数未检测到常见的融合基因。结论CAG方案对复发或初次诱导缓解治疗失败的T-ALL有效,但仍需扩大病例数进一步验证并探索其作用机制。

参麦注射液对慢性萎缩性胃炎患者外周血T淋巴细胞亚群功能改善的研究

目的:探讨参麦注射液对慢性萎缩性胃炎(CAG)患者T淋巴细胞亚群功能的影响。方法:以50例CAG患者和35例健康志愿者为研究对象,用流式细胞仪(flow cytometer;FCM)进行T淋巴细胞亚群功能的测定,并比较CAG患者治疗前后T淋巴细胞亚群的变化情况。结果:CAG患者T淋巴细胞亚群CD4+、CD4+/CD8+值都低于正常对照组,Hp菌阳性者显著低于Hp菌阴性者、正常人(P<0.01)。结论:CAG患者存在T淋巴细胞亚群比例失调,应用参麦注射液治疗后可改善T淋巴细胞亚群功能。