In vitro biofilm formation in ESBL producing Escherichia coli isolates from cage birds

Objective:To determine biofilm and hydrophobicity formation ratios in extended spectrum beta lactamases(ESBL) synthesizing Escherichia coli isolates which were isolated from feces samples of 150 cage bird species randomly taken from pet shops in Hatay province,Turkey.Methods:In vitro biofilm production of 4 ESBL positive isolates were performed by Congo Red Agar(CRA),Standard Tube(ST) and Microtitre Plate(MP) methods while their hydrophobicity were examined by bacterial adhesion to hydrocarbon(BATH) test.Results:In the examined isolates,while biofilm production was found to be negative by CRA method,highest biofilm producing strain,among 4 bacteria was determined to be A42 by ST and MP methods.The Scanning Electron Microscopy(SEM) also displayed these confirmed findings.The hydrophobicity values of strains were determined to be between 22.45%and 26.42%.Conclusions:As a result,biofilm formation in cage bird feces originated ESBL positive Escherichia coli isolates was performed for the first time in Turkey.In order to present the relation between pathogenicity and biofilm production in animal originated ESBL positive isolates,further studies are required.

Analysis of Symbolism of the Caged Bird in the Novel --Is He Living or Is He Dead？

As one of Mark Twain＇ s most classic short story, ＂ Is He Living or Is He Dead＂ does not reflect the major problems and the serious drawbacks of society fi＇om the surface significance of novel, however, the events described in the novel can actually touch sensitive topic as well as the shortcomings of the era where, pointedly disclose the nature of society at that time. Mark Twain＇s short story tries to use this caged bird imagery to criticize the camouflage events occur and makes irony of this tragic story behind the social problems. Little caged bird in the novel contains profound social implications and is worthy of scrutiny and research.