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Modeling the Drying Kinetics of Pigeon Pea [Cajanus cajan (L.) Millspaugh]
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作者 Nadia Pamela Gladys Pambou-Tobi Arnaud Wenceslas Geoffroy Tamba Sompila +3 位作者 Michel Elenga Reyes Herdenn Gampoula Gloire Horiane Louya Banzouzi Sylvia Petronille Ntsossani 《Open Journal of Applied Sciences》 2024年第6期1425-1436,共12页
We set out to model the oven-drying kinetics of a legume known as pigeon pea, harvested in the Bouenza department in the south-west of the Republic of Congo. The drying kinetics of pigeon peas was carried out in an ov... We set out to model the oven-drying kinetics of a legume known as pigeon pea, harvested in the Bouenza department in the south-west of the Republic of Congo. The drying kinetics of pigeon peas was carried out in an oven under experimental conditions using temperatures of: 50°C, 60°C and 70°C. Seven mathematical models were used to describe pigeon pea drying. During drying, water loss was faster and shorter at 70°C [10.446 g/25 g wet weight (wwb) for 320 min (5.3 h)] compared to 50°C [10.996 g/25 g wet weight (wwb) for 520 min (8.6 h)] and 60°C [10.616 g/25 g wet weight (wwb) for 420 min (7.0 h)] where it was slower and longer. With regard to modeling, and based on the principle of choosing the right model focusing on the high value of R2 and low values of χ2 and RMSE, two models were selected, the Midili model for temperatures of 50°C and 60°C and the Henderson and Pabis model modified for temperature of 70°C showed better results. The R2, χ2 and RMSE values calculated for pigeon pea are 0.99985, 3.93404E-5 and 0.00627;0.9997, 9.245E-5 and 0.00962;0.99996, 1.56332E-5 and 0.00395 respectively at 50°C, 60°C and 70°C. 展开更多
关键词 cajanus cajan lEGUME Kinetic Models DRYING
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A Comprehensive Transcriptome Assembly of Pigeonpea (Cajanus cajan L.) using Sanger and Second-Generation Sequencing Platforms
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作者 Himabindu Kudapa Arvind K. Bharti +16 位作者 Steven B. Cannon Andrew D. Farmer Benjamin Mulaosmanovi Robin Kramer Abhishek Bohra Nathan T. Weeks John A. Crow Reetu Tuteja Trushar Shah Sutapa Dutta Deepak K. Gupta Archana Singh Kishor Gaikwad Tilak R. Sharma Gregory D. May Nagendra K. Singh Rajeev K. Varshney 《Molecular Plant》 SCIE CAS CSCD 2012年第5期1020-1028,共9页
A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA IIx single end reads, 2.19 million single end FLX/454 reads, and 18 353 Sanger expressed sequenced t... A comprehensive transcriptome assembly for pigeonpea has been developed by analyzing 128.9 million short Illumina GA IIx single end reads, 2.19 million single end FLX/454 reads, and 18 353 Sanger expressed sequenced tags from more than 16 genotypes. The resultant transcriptome assembly, referred to as CcTA v2, comprised 21 434 transcript as- sembly contigs (TACs) with an N50 of 1510 bp, the largest one being -8 kb. Of the 21 434 TACs, 16 622 (77.5%) could be mapped on to the soybean genome build 1.0.9 under fairly stringent alignment parameters. Based on knowledge of intron junctions, 10 009 primer pairs were designed from 5033 TACs for amplifying intron spanning regions (ISRs). By using in silico mapping of BAC-end-derived SSR loci of pigeonpea on the soybean genome as a reference, putative mapping posi- tions at the chromosome level were predicted for 6284 ISR markers, covering all 11 pigeonpea chromosomes. A subset of 128 ISR markers were analyzed on a set of eight genotypes. While 116 markers were validated, 70 markers showed one to three alleles, with an average of 0.16 polymorphism information content (PIC) value. In summary, the CcTA v2 transcript assembly and ISR markers will serve as a useful resource to accelerate genetic research and breeding applications in pigeonpea. 展开更多
关键词 cajanus cajan l.) second-generation sequencing transcriptome assembly intron spanning region (ISR)markers.
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A Study on Comparative Fertility Restoration in A2 and A4 Cytoplasms and Its Implication in Breeding Hybrid Pigeonpea [Cajanus cajan (L.) Millspaugh]
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作者 Arbind K. Choudhary Indra Prakash Singh 《American Journal of Plant Sciences》 2015年第2期385-391,共7页
Exploitation of hybrid vigour has been visualized as the most efficient option for increasing productivity in pigeonpea [Cajanus cajan (L.) Millspaugh]. Cytoplasms from various wild relatives of pigeonpea have been tr... Exploitation of hybrid vigour has been visualized as the most efficient option for increasing productivity in pigeonpea [Cajanus cajan (L.) Millspaugh]. Cytoplasms from various wild relatives of pigeonpea have been transferred to develop CMS lines in the background of cultivated pigeonpea. However, A2 (Cajanus scarabaeoides) and A4 (Cajanus cajanifolius) cytoplasms have been utilized most frequently. In order to study fertility restoration efficiency in F1 hybrids having either A2 or A4 cytoplasms, an experiment was conducted at the Indian Institute of Pulses Research (IIPR), Kanpur during 2008-2012. Four CMS lines namely Hy4A, H28A (each with A2 cytoplasm), ICP 2039A and ICP 2043A (both with A4 cytoplasm) were crossed with ten genotypes/restorers of long duration pigeonpea for two years. The F1 hybrids so-obtained were assessed in the succeeding years for pollen fertility and pod setting. All the pollinators except IPA 203 restored fertility in F1 hybrids derived from ICP 2039A and ICP 2043A (both having A4 cytoplasm). However, none of the restorers were effective in restoring fertility in hybrids derived from Hy4A and H28A (each with A2 cytoplasm). This could be ascribed to undesirable linkage drag still present in these two CMS lines having A2 cytoplasm. The F2 progenies derived from 4 hybrids (ICP 2039A × NA-1, ICP 2039A × Bahar, ICP 2043A × NA-1 and ICP 2043A × Bahar) segregated approximately into 3 fertile: 1 sterile plants. However, 2 F2 progenies having Pusa 9 as the restorer revealed approximately 15 fertile:1 sterile ratio. Thus monogenic and digenic duplicate gene action with complete dominance for fertility restoration was observed in F1 hybrids derived from CMS lines having A4 cytoplasm. F3 progenies from individual F2 plants of these crosses also confirmed the same pattern of fertility restoration. This study indicated that CMS lines based on A4 cytoplasm would be more desirable as these might have more number of restorers compared to those having A2 cytoplasm. 展开更多
关键词 cajanus cajan CMS lines A4 CYTOPlASM FERTIlITY Restoration HYBRID PIGEONPEA
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Amelioration of Aluminium Toxicity in Pigeon Pea [Cajanus cajan (L.) Millsp.] Plant by 24-Epibrassinolide
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作者 N. Divya Sri M. Madhan Mohan +2 位作者 K. Mahesh K. Raghu S. Seeta Ram Rao 《American Journal of Plant Sciences》 2016年第12期1618-1628,共11页
The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhib... The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhibitory impact of aluminium toxicity on root growth which was further manifested in overall improvement of vegetative growth. Application of 24-epibrassinolide removed the inhibitory influence of Al nodulation. The growth stimulation in Cajanus plants by 24-EBL under Al stress was associated with elevated levels of chlorophylls, nucleic acids and soluble proteins. 24-Epibrassinolide application enhanced proline content in Al<sup>3+</sup> stressed Cajanus plants. Further, the supplementation of 24-epibrassinolide to Al stress treatments increased the activities of antioxidative enzymes viz., catalase [EC 1.11.1.6];peroxidase [EC 1.11.1.7];superoxide dismutase [EC 1.15.1.1] and ascorbate peroxidase [EC 1.11.1.11]. Lipid peroxidation induced by Al was found reduced with the supplementation of 24-epibrassinolide. The present studies demonstrated the ameliorating capability of 24-epibrassinolide on the Al induced inhibition of plant growth of C. cajan. 展开更多
关键词 Aluminium Toxicity 24-EPIBRASSINOlIDE cajanus cajan Antioxidative Enzymes
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不同木豆幼苗低温胁迫下的生理响应及耐寒性鉴定
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作者 唐军 王文强 +3 位作者 丁西朋 马向丽 毕玉芬 郭凤根 《热带作物学报》 CSCD 北大核心 2024年第8期1633-1640,共8页
木豆[Cajanus cajan (L.) Millsp.]为热区重要的粮饲兼用作物。为解析木豆响应低温胁迫生理特性及耐寒能力,本研究以8份木豆种质幼苗为试验材料,通过低温胁迫(4℃)处理,对木豆8个生理指标测定分析,运用隶属函数综合分析法鉴定其耐寒性... 木豆[Cajanus cajan (L.) Millsp.]为热区重要的粮饲兼用作物。为解析木豆响应低温胁迫生理特性及耐寒能力,本研究以8份木豆种质幼苗为试验材料,通过低温胁迫(4℃)处理,对木豆8个生理指标测定分析,运用隶属函数综合分析法鉴定其耐寒性。结果表明:随低温胁迫时间的延长,木豆相对电导率(REC)和可溶性糖(SS)、丙二醛(MDA)、脯氨酸(Pro)的含量呈升高趋势,超氧化物气化酶(SOD)、过氧化物酶(POD)活性随低温处理时间的延长呈先升后降之势,叶绿素(Chl)含量总体呈下降趋,过氧化氢酶(CAT)活性变化存在差异,仅P_(2)中CAT活性差异显著。运用隶属函数综合评价木豆耐寒性强弱为:缅甸木豆>海南儋州木豆>木豆(170306001)>木豆(060302216)>ICPL7086>木豆(南02137)>广西木豆>海南琼中木豆,且木豆耐寒性与Chl含量、SOD和POD活性呈显著相关,这些指标可以作为木豆耐寒性评价的可信指标。 展开更多
关键词 木豆 低温胁迫 生理指标 耐寒性
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岭南常用中药木豆叶中牡荆苷的HPLC含量测定 被引量:9
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作者 李功营 唐洪梅 +2 位作者 丘振文 黄月纯 张映娜 《中药新药与临床药理》 CAS CSCD 2007年第6期474-476,共3页
目的建立木豆叶中牡荆苷的含量测定方法。方法色谱柱为Hypersiol ODS2 C18柱(250mm×4.6mm,5μm);流动相:0~20min,甲醇∶1%醋酸(25∶75),20min后,甲醇∶1%醋酸(30∶70);流速:1.0mL/min;检测波长:339nm。结果牡荆苷在0.1044~0.522... 目的建立木豆叶中牡荆苷的含量测定方法。方法色谱柱为Hypersiol ODS2 C18柱(250mm×4.6mm,5μm);流动相:0~20min,甲醇∶1%醋酸(25∶75),20min后,甲醇∶1%醋酸(30∶70);流速:1.0mL/min;检测波长:339nm。结果牡荆苷在0.1044~0.522μg范围内线性关系良好,r=0.9994,平均回收率为100.11%,RSD为0.63%。结论该法简便,重复性、稳定性良好,可作为木豆叶中牡荆苷含量测定方法。 展开更多
关键词 木豆叶 牡荆苷 HPlC 含量测定
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饲粮中添加鲜木豆叶粉对育肥猪生长发育的影响
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作者 王友印 王志勇 +5 位作者 罗灵 王坚 王目森 喻珊 廖丽 欧文军 《饲料研究》 CAS 北大核心 2024年第1期21-28,共8页
试验旨在探究鲜木豆叶粉对育肥猪生长性能、养殖经济效益、屠宰性能、肉品质及肠道微生物的影响。选取24头同批次健康、体重相近的海南黑猪,随机分为4组,每组6个重复,每个重复1头猪。对照组(A组)育肥猪饲喂基础饲粮,试验B组、C组和D组... 试验旨在探究鲜木豆叶粉对育肥猪生长性能、养殖经济效益、屠宰性能、肉品质及肠道微生物的影响。选取24头同批次健康、体重相近的海南黑猪,随机分为4组,每组6个重复,每个重复1头猪。对照组(A组)育肥猪饲喂基础饲粮,试验B组、C组和D组分别在基础饲粮中添加10%、15%和20%鲜木豆叶粉。预试期7 d,正式试验期270 d。结果显示,与对照组相比,各试验组育肥猪的平均日采食量均显著下降(P<0.05),试验D组育肥猪的平均日增重和末重显著下降(P<0.05)。试验C组养殖利润分别比对照组、试验B组和试验D组增加6.36%、5.68%和9.99%。与对照组相比,各试验组育肥猪的背膘厚度均显著降低(P<0.05),肉色评分均显著升高(P<0.05);试验B组和C组猪肉的pH24 h值显著升高(P<0.05);各试验组背最长肌的总氨基酸、总必需氨基酸和鲜味氨基酸含量显著升高(P<0.05);试验C组和D组饱和脂肪酸含量显著降低(P<0.05),单不饱和脂肪酸和不饱和脂肪酸含量显著升高(P<0.05);试验D组多不饱和脂肪酸含量显著升高(P<0.05)。与对照组相比,试验B组拟杆菌门相对丰度增大(P<0.05),试验B组和C组变形菌门相对丰度降低(P<0.05);各试验组大肠杆菌志贺氏属相对丰度降低(P<0.05)。研究表明,饲粮中添加鲜木豆叶粉可改善育肥猪屠宰性能、肉品质和风味,优化肠道微生物结构,减少脂肪沉积和有害菌相对丰度,以添加15%鲜木豆叶粉效果最优。 展开更多
关键词 鲜木豆叶粉 育肥猪 生长性能 屠宰性能 肉品质 肠道微生物
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两份海南地方木豆资源的核型分析
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作者 程志号 孙长君 +3 位作者 郭素霞 曾学蔚 严琳玲 吴琼 《草业科学》 CAS CSCD 北大核心 2024年第7期1645-1649,共5页
木豆(Cajanus cajan)是我国热带亚热带地区重要的豆类作物,是一种集粮、饲料、药物为一体的多用途植物,同时,作为先锋植物,在水土保持、土壤改良等方面有重要功能。海南木豆种植时间长、资源多样性高,但细胞遗传学方面的报道目前较少。... 木豆(Cajanus cajan)是我国热带亚热带地区重要的豆类作物,是一种集粮、饲料、药物为一体的多用途植物,同时,作为先锋植物,在水土保持、土壤改良等方面有重要功能。海南木豆种植时间长、资源多样性高,但细胞遗传学方面的报道目前较少。本研究以海南两个重要木豆产区‘保亭’和‘白沙’木豆地方资源为材料,通过核型分析发现,‘保亭’木豆核型公式为2n=2x=22=16m+6sm,其中10号染色体有一对随体,相对长度组成为2n=1L+5M2+3M1+2s,为1A型;‘白沙’木豆核型公式为2n=2x=22=20m+2sm,未发现随体,相对长度组成为2n=1L+4M2+5M1+1s,为2A型。本研究结果为木豆资源鉴定提供了新技术,也为种质创新和新品种选育提供参考。 展开更多
关键词 木豆 资源 核型分析 随体 对称性
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木豆WD 40基因家族鉴定及响应茉莉酸甲酯的表达分析
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作者 牛禹极 吴锦林 +3 位作者 陈耀阳 林岩松 杨杰 付玉杰 《石河子大学学报(自然科学版)》 CAS 北大核心 2024年第1期36-46,共11页
WD40蛋白参与调控植物生长发育、次生代谢产物合成及胁迫应答等生物学过程。WD40主要作为MYB-bHLH-WD40(MBW)蛋白复合体成员之一,激活花青素合成下游基因转录进而促进花青素的积累。探究WD 40基因家族的功能是解析黄酮类次生代谢产物代... WD40蛋白参与调控植物生长发育、次生代谢产物合成及胁迫应答等生物学过程。WD40主要作为MYB-bHLH-WD40(MBW)蛋白复合体成员之一,激活花青素合成下游基因转录进而促进花青素的积累。探究WD 40基因家族的功能是解析黄酮类次生代谢产物代谢调控机制的关键环节。本研究基于木豆(Cajanus cajan)基因组鉴定了木豆CcWD40家族成员,并对CcWD 40基因进行生物信息学分析以及对茉莉酸甲酯响应的实验。利用生物信息学在木豆基因组中鉴定出116个CcWD 40基因家族成员,系统全面地评价了候选基因的基因结构、染色体分布、启动子顺式作用元件及系统发育进化历程等特征,并分析了其在茉莉酸甲酯处理下的表达模式。结果表明:WD40蛋白质包含的氨基酸残基数目在296~1709之间,等电点范围为4.33~9.58;116个基因可进行定位的基因有68个,这68个CcWD 40基因不均匀分布于11条染色体上,大多位于3号染色体;系统进化树将木豆CcWD 40家族成员分为18个亚家族,尽管其基因结构间内含子差异较大,但在进化树同一分支中的较为相似;共线性分析显示,在所选3种模式植物中,木豆与大豆亲缘关系最近;木豆CcWD 40基因具有多个响应元件,包括胁迫响应元件、发育响应元件和激素响应元件,如脱落酸响应元件(ABRE)、水杨酸响应元件(TCA-element)、赤霉素响应元件(GARE-Motif)等,可见它们的表达受到复杂的调控网络的控制,可能在非生物胁迫中起到重要作用;基于RNA-seq数据分析木豆CcWD40家族基因应茉莉酸甲酯的表达特征,其中96个CcWD 40基因的表达量会在MeJA处理后首先逐渐降低,只有20个CcWD 40基因的表达量会首先呈现升高趋势,并且同一进化分支的CcWD 40基因相应趋势大体相同。该研究将为进一步探索WD 40基因在调控黄酮类化合物合成和响应非生物胁迫应答中的功能提供重要基因资源和理论基础。 展开更多
关键词 木豆 CcWD 40 全基因组鉴定 茉莉酸甲酯
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木豆叶HPLC指纹图谱建立及2种成分测定 被引量:1
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作者 胥爱丽 毕晓黎 +3 位作者 李素梅 李养学 江洁怡 刘布鸣 《中成药》 CAS CSCD 北大核心 2017年第7期1435-1439,共5页
目的建立木豆Cajanus cajan(L.)Millsp.叶HPLC指纹图谱,并测定荭草苷和木犀草素的含有量。方法木豆叶65%甲醇提取液的分析采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);以甲醇-1%醋酸为流动相,梯度洗脱;体积流量1.0 m L/m... 目的建立木豆Cajanus cajan(L.)Millsp.叶HPLC指纹图谱,并测定荭草苷和木犀草素的含有量。方法木豆叶65%甲醇提取液的分析采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);以甲醇-1%醋酸为流动相,梯度洗脱;体积流量1.0 m L/min;柱温25℃;检测波长260 nm。结果 10批样品(S1~S10)HPLC指纹图谱中有21个共有峰,除S3(0.516)外,其相似度均大于0.950。荭草苷和木犀草素分别在0.089 5~3.960μg和0.015 5~0.408μg范围内呈良好的线性关系,平均加样回收率分别为99.43%(RSD=1.32%)和98.50%(RSD=0.82%),3个产地(广东、云南、海南)样品中2种成分含有量有明显差异。结论该方法简单准确,重复性好,可用于木豆叶的质量控制。 展开更多
关键词 木豆 HPlC指纹图谱 荭草苷 木犀草素
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木豆叶、鲜地黄提取物对ox-LDL损伤的HUVEC的保护作用 被引量:2
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作者 刘长河 李开言 +5 位作者 张雪侠 王艳艳 李华妮 葛文静 刘方洲 王晓丽 《中药新药与临床药理》 CAS CSCD 北大核心 2021年第8期1073-1078,共6页
目的研究木豆叶、鲜地黄提取物(DMT)对氧化低密度脂蛋白(ox-LDL)诱导损伤的人脐静脉内皮细胞(HUVEC)的影响,初步探讨DMT防治动脉粥样硬化的机制。方法应用血清药理学方法制备正常对照、辛伐他汀、DMT血清,以供细胞实验使用。采用ox-LDL... 目的研究木豆叶、鲜地黄提取物(DMT)对氧化低密度脂蛋白(ox-LDL)诱导损伤的人脐静脉内皮细胞(HUVEC)的影响,初步探讨DMT防治动脉粥样硬化的机制。方法应用血清药理学方法制备正常对照、辛伐他汀、DMT血清,以供细胞实验使用。采用ox-LDL损伤HUVEC,与各组药物共培养,即空白组、模型组、辛伐他汀组(15%辛伐他汀血清)及DMT低(5%DMT血清)、中(10%DMT血清)、高剂量组(15%DMT血清),24 h后MTT法检测细胞活力;收集细胞,流式细胞术检测细胞内活性氧(ROS)含量;硝酸还原酶法检测上清液NO含量;ELISA法检测上清液内皮素(ET-1)、细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)含量;Western Blot法检测细胞凝集素样氧化低密度脂蛋白受体(Lox-1)、Caspase-3、Cytc蛋白的表达。结果与模型组比较,辛伐他汀组及DMT低、中、高剂量组细胞活力明显提高(P<0.01);DMT低剂量组ROS水平明显降低(P<0.05);辛伐他汀组细胞上清液ET-1、VCAM-1含量及Lox-1、Caspase-3、Cytc蛋白含量明显降低(P<0.05,P<0.01);DMT低剂量组细胞Caspase-3、Cytc蛋白含量明显降低(P<0.01);DMT中剂量组细胞上清液ET-1、VCAM-1含量及Lox-1、Caspase-3、Cytc蛋白含量明显降低(P<0.05,P<0.01),细胞上清液中NO含量明显升高(P<0.01);DMT高剂量组细胞上清液ET-1、VCAM-1含量及Lox-1、Cytc蛋白含量明显降低(P<0.01),NO含量明显升高(P<0.01)。结论DMT能够抑制ox-LDL诱导的HUVEC损伤,恢复其收缩舒张分泌功能,其机制可能与影响Lox-1介导的内皮细胞氧化应激有关。 展开更多
关键词 木豆叶 鲜地黄 氧化低密度脂蛋白(ox-lDl) 人脐静脉内皮细胞(HUVEC) 细胞凝集素样氧化低密度脂蛋白受体(lox-1) 氧化应激
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Influence of Water Content on the Quality of Pigeonpea Seeds 被引量:1
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作者 Cristiane Fernandes Lisboa Rodrigo Starnek Lopes De Araújo +7 位作者 Itamar Rosa Teixeira José Hortêncio Mota Alessandro Guerra Da Silva Marcos Eduardo Viana De Araújo Deyner Damas Aguiar Silva Edgar Estevam Franca Isneider Luiz Silva Fernando Ribeiro Teles De Camargo 《American Journal of Plant Sciences》 2017年第10期2397-2406,共10页
The species Cajanus cajan L. where pigeonpea is from presents a harvest potential in tropical regions for it is used as human and animal feed, besides being used as green fertilizer. However, the harvest area of this ... The species Cajanus cajan L. where pigeonpea is from presents a harvest potential in tropical regions for it is used as human and animal feed, besides being used as green fertilizer. However, the harvest area of this Fabaceae is still insignificant, due to especially the lack of quality seeds. In this context, it is possible to affirm that drying and store are portrayed as important steps for obtaining superior quality seeds. The aim of this study is to evaluate the physiologic quality of dwarf pigeonpea seeds, with different water content during storage. A factorial scheme 3 × 6 was adopted in the delimitation completely randomized, with four replications. Treatments were constituted by the combination of lots of seeds containing three different water contents (11%, 14% and 16%), submitted to a 10-month storage period, with evaluations every two months (0, 2, 4, 6, 8 and 10). Seeds were stocked in a bag type kraft under normal lab conditions, that is, no control. Physiologic quality of the seeds was evaluated through the following tests: Germination Pattern Test—GPT, first count of germination, accelerated aging and electric conductivity. It can be concluded that pigeonpea with hard seeds containing water content at 11% existing superior physiologic quality throughout the 10-month storage, under no controlled condition, certainly promoted by the less intense breathing from the reserves cumulated in the seed lot. Pigeonpea seeds storage with water content superior to 14% promotes a sharp decrease of physiologic quality, due to an increase in metabolic activity. 展开更多
关键词 cajanus cajan l. Physiologic Deterioration Seed Quality Viability and Vigour
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干旱胁迫对木豆叶片的影响及机理验证
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作者 魏一凡 杨清 +4 位作者 宋治华 董碧莹 曹红燕 代阳 孟冬 《东北林业大学学报》 CAS CSCD 北大核心 2024年第9期36-45,共10页
以木豆(Cajanus cajan)为研究对象,运用生物信息学方法从木豆的基因组中鉴定基因,对其进行了基本的理化性质预测、系统发育、保守基序、顺式作用元件等分析,同时进行组织特异性分析、过表达植株构建以及生理指标分析。结果表明:从木豆... 以木豆(Cajanus cajan)为研究对象,运用生物信息学方法从木豆的基因组中鉴定基因,对其进行了基本的理化性质预测、系统发育、保守基序、顺式作用元件等分析,同时进行组织特异性分析、过表达植株构建以及生理指标分析。结果表明:从木豆基因组中鉴定得到14个SnRK2家族基因,发现SnRK2氨基酸的数量在217~383之间,相对分子质量为24578.32~43627.79,等电点范围为4.78~9.22;SnRK2基因家族聚集成3个分支,分布均匀;外显子和内含子分布保守,除CcSnRK2.13(8个外显子和7个内含子)和CcSnRK2.14(6个外显子和5个内含子)外,都含有9个外显子以及8个内含子;所有的CcSnRK2基因启动子区域都包含启动子和增强子区、厌氧诱导、光诱导调控、玉米蛋白代谢诱导调控、干旱诱导调控和脱落酸诱导调控元件;CcSnRK2家族成员的二级结构在一定程度上有相似性,每个结构的氨基酸数量占比较为一致;根据基因表达情况以及前面的分析结果,选择CcSnRK2.5、CcSnRK2.8和CcSnRK2.9进行功能验证,发现其在叶片中高表达。CcSnRK2.5、CcSnRK2.8和CcSnRK2.9过表达显著提高了植株的耐旱生存能力,保持较好的叶片形态、水分平衡、细胞膜完整性等,表现出干旱胁迫条件下生理指标的显著改善。从全基因组鉴定了SnRK2基因并表征了其基本特征,通过植物遗传转化发现SnRK2增强了木豆耐旱性。 展开更多
关键词 木豆 干旱胁迫 SnRK2 生物信息学 表达分析
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森林植物木豆CcGRFs全基因组鉴定及耐盐功能
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作者 武模征 王胜杰 +5 位作者 代阳 陈杨 张修齐 吴睿 孟冬 杨清 《东北林业大学学报》 CAS CSCD 北大核心 2024年第8期14-22,共9页
从木豆(Cajanus cajan)全基因组中鉴定出CcGRF家族的全部成员CcGRF1~CcGRF10,系统发育分析显示所有的CcGRFs聚类为5组,基序分析显示CcGRF家族每个成员都具有谷氨酰胺-亮氨酸-谷氨酰胺(QLQ)结构域和色氨酸-精氨酸-半胱氨酸(WRC)结构域。... 从木豆(Cajanus cajan)全基因组中鉴定出CcGRF家族的全部成员CcGRF1~CcGRF10,系统发育分析显示所有的CcGRFs聚类为5组,基序分析显示CcGRF家族每个成员都具有谷氨酰胺-亮氨酸-谷氨酰胺(QLQ)结构域和色氨酸-精氨酸-半胱氨酸(WRC)结构域。对启动子区域的顺式作用元件预测,发现CcGRFs启动子序列中包含胁迫相关的顺式作用元件。利用荧光定量PCR技术检测CcGRFs基因的组织特异性及其在NaCl处理不同时间时的表达模式,结果显示:CcGRF10在各个组织中的表达水平均显著高于其他CcGRF基因,且盐处理下CcGRF10的表达持续上调。对木豆幼苗进行不同浓度NaCl处理,发现在75 mmol/L NaCl处理后木豆叶片黄化,木豆的存活率和叶片相对含水量降低,叶片卷曲指数升高。通过农杆菌介导的瞬时转化及发状根(Hairy Root)稳定转化的方法在木豆中过表达CcGRF10。NaCl处理后,CcGRF10-OE株系的存活率、叶片相对含水量显著高于EV株系,叶片卷曲指数和丙二醛(MDA)质量摩尔浓度显著低于EV株系,表明过表达CcGRF10能够提高木豆植株的耐盐性。通过观察CcGRF10的亚细胞定位,发现CcGRF10定位于细胞核,与生物信息学分析结果一致。 展开更多
关键词 木豆 CcGRFs 转基因 非生物胁迫 盐胁迫
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Conventional and Molecular Approaches towards Genetic Improvement in Pigeonpea for Insects Resistance 被引量:1
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作者 Arbind K. Choudhary Ranjeet Sharad Raje +2 位作者 Subhojit Datta Rafat Sultana Timmanna Ontagodi 《American Journal of Plant Sciences》 2013年第2期372-385,共14页
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the chara... Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the characteristic feature of this crop. This is primarily ascribed to its susceptibility/sensitivity to a number of biotic and abiotic factors. Among biotic factors, insects such as pod borer (Helicoverpa armigera), pod fly (Melanoagromyza obtusa) and spotted borer (Maruca vitrata) substantially damage the crop and result in significant economic losses. Management of these insects by genetic means has always been considered environment friendly approach. However, genetic improvement has always been impeded by limited genetic variability in the primary gene pool of pigeonpea. Wild species present in the secondary and tertiary gene pools have been reported to carry resistance for such insects. However, transfer of resistance through conventional backcrossing has not been much successful. It calls for gene introgression through marker assisted backcrossing (MABC) or advanced backcross breeding (AB breeding). In this review, we have attempted to assess the progress made through conventional and molecular breeding and suggested the ways to move further towards genetic enhancement for insects resistance in 展开更多
关键词 cajanus cajan INSECTS RESISTANCE Wild Species Secondary Gene POOl MABC AB Breeding
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一株特异性产荭草苷木豆内生真菌Fusarium solani RFF的分离纯化及鉴定 被引量:3
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作者 韩海燕 张智慧 +3 位作者 唐佩佳 郑文艺 顾成波 罗朝晖 《黑龙江医药》 CAS 2020年第5期979-982,共4页
目的:从药用植物木豆中分离纯化特异性产荭草苷的内生真菌,扩大荭草苷的药源。方法:采用组织块分离法分离木豆根内生真菌,利用HPLC法和LC-ESI-MS法对内生真菌的发酵产物进行鉴定,并通过形态观察及ITS-rDNA法对特异性产红草苷的内生真菌... 目的:从药用植物木豆中分离纯化特异性产荭草苷的内生真菌,扩大荭草苷的药源。方法:采用组织块分离法分离木豆根内生真菌,利用HPLC法和LC-ESI-MS法对内生真菌的发酵产物进行鉴定,并通过形态观察及ITS-rDNA法对特异性产红草苷的内生真菌进行种属鉴定。结果:内生真菌RFF可特异性产红草苷,菌落呈现白色,短绒毛状,分生孢子呈弯曲镰刀,孢子有2~3隔;ITS序列与Fusarium solani相应序列的相似性为100%。结论:从药用植物木豆中分离到一株特异性产荭草苷的内生真菌RFF,鉴定为腐皮镰孢菌(Fusarium solani)。 展开更多
关键词 木豆内生真菌 分离 鉴定 荭草苷
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Quality nutrition through pigeonpea—a review
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作者 Kul Bhushan Saxena Ravikoti Vijaya Kumar Rafat Sultana 《Health》 2010年第11期1335-1344,共10页
Protein mal-nutrition is widespread among poor of developing and under developed countries. Since animal protein is beyond the reach of this group, their primary protein supply comes from plant based products. Amongst... Protein mal-nutrition is widespread among poor of developing and under developed countries. Since animal protein is beyond the reach of this group, their primary protein supply comes from plant based products. Amongst these, pigeonpea or red gram (Cajanus cajan (L.) Millspaugh) is an important food legume that can be grown under rainfed conditions with least inputs. Pigeonpea is rich in starch, protein, calcium, manganese, crude fiber, fat, trace elements, and minerals. Besides its high nutritional value, pigeonpea is also used as traditional folk medicine in India, China, Philippines and some other nations. Literature on this aspect show that pigeonpea is capable to prevent and cure a number of human ailments such as bronchitis, coughs, pneumonia, respiratory infections, dysentery, menstrual disorders, sores, wounds, abdominal tumors, tooth ache, and diabetes. 展开更多
关键词 PROTEIN PIGEONPEA cajanus cajan NUTRITION FOlK MEDICINE
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Antibacterial activity of naringenin-rich fraction of pigeon pea leaves toward Salmonella thypi
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作者 Sarifa Agus Suminar Setiati Achmadi Nisa Rachmania Mubarik 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2017年第8期725-728,共4页
To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).MethodsThe leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extrac... To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).MethodsThe leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby-Bauer method.ResultsSubfraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol.ConclusionsNaringenin shows antibacterial activity and can be developed to treat typhoid. 展开更多
关键词 Antibacterial activity cajanus cajan FlAVANONES NARINGENIN Salmonella thypi TYPHOID
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木豆种子和荚的化学成分及其抗氧化、抗炎活性 被引量:2
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作者 徐晓俞 李程勋 李爱萍 《林业科学》 EI CAS CSCD 北大核心 2023年第2期67-74,共8页
【目的】对木豆种子和荚部位的化学成分进行分离鉴定,并研究这些化学成分的抗氧化、抗炎活性,以期为发现新的天然抗氧化、抗炎活性成分以及木豆资源的开发利用提供科学依据。【方法】分别采用95%乙醇回流提取木豆种子和荚,提取液过滤后... 【目的】对木豆种子和荚部位的化学成分进行分离鉴定,并研究这些化学成分的抗氧化、抗炎活性,以期为发现新的天然抗氧化、抗炎活性成分以及木豆资源的开发利用提供科学依据。【方法】分别采用95%乙醇回流提取木豆种子和荚,提取液过滤后减压浓缩得到木豆种子和荚的浸膏。浸膏分别用不同有机溶剂萃取,对乙酸乙酯萃取部继续采用硅胶、凝胶等柱层析色谱分离技术进行进一步的分离、纯化。利用MS、NMR等技术鉴定分离得到的化合物的结构。通过测定化合物DPPH自由基清除能力评价化合物的抗氧化活性,采用Griess法测定化合物对脂多糖诱导小鼠巨噬细胞RAW 264.7产生NO的抑制能力评价化合物的抗炎活性。【结果】从木豆种子乙酸乙酯萃取部中分离出4个化合物,通过ESI-MS、NMR等技术鉴定为对苯二酚(S1)、1–亚油酰–3–棕榈酰甘油(S2)、胡萝卜苷亚油酸酯(S3)、豆甾烷–3β,6α–二醇(S4)。从木豆荚乙酸乙酯萃取部中分离出5个化合物,根据理化性质初步鉴定为黄酮类化合物,通过ESI-MS、NMR等技术鉴定为2’,5,7–三羟基–4’–甲氧基异黄酮(P1)、毛蕊异黄酮(P2)、大豆苷元(P3)、2’-羟基染料木素(P4)、染料木素(P5)。化合物S1~S4、P1~P2为首次从木豆属植物中分离得到,其中化合物S2为新化合物。抗氧化和抗炎活性研究表明,化合物S1、 P1、 P2、 P4具有较强的DPPH自由基清除活性,EC50值分别达到(63.10±0.15)、(64.97±0.67)、(79.63±1.14)和(79.43±1.29)μmol·L^(-1),化合物S1~S2、 P1~P5具有较强的NO抑制活性,IC50值分别为(31.36±0.44)、(52.08±1.11)、(30.66±0.83)、(46.30±0.90)、(55.18±2.07)、(44.11±0.79)和(44.92±2.24)μmol·L^(-1)。化合物P1的抗氧化和抗炎活性以及化合物P4的抗炎活性为首次报道。【结论】木豆种子中主要含脂肪酸及甾醇衍生物,抗氧化和抗炎活性较弱,木豆荚中含有的多种异黄酮类成分具有较好的抗氧化和抗炎活性。因此,木豆荚更具有开发利用价值,可作为天然抗氧化及抗炎成分的提取原料来源。 展开更多
关键词 木豆种子 化学成分 异黄酮 抗氧化活性 抗炎活性
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Physiological Quality of Pigeonpea Seed after Application of Desiccant Herbicides
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作者 Raniele Tadeu Guimaraes Itamar Rosa Teixeira +4 位作者 André José Campos Gisele Carneiro da Silva Ivano Alessandro Devilla Alessandro Guerra da Silva Paulo César Timossi 《American Journal of Plant Sciences》 2015年第6期826-832,共7页
The pre-harvest application of herbicides may impair seed quality. This way, this paper was conducted to evaluate the effects of the application of desiccant herbicides on the physiological quality of pigeonpea seeds.... The pre-harvest application of herbicides may impair seed quality. This way, this paper was conducted to evaluate the effects of the application of desiccant herbicides on the physiological quality of pigeonpea seeds. Six batches of seeds from plants desiccated with glyphosate were evaluated at doses: 1.125 (B1), 1.5 (B2) and 1.875 L&middotha-1 (B3);and Gramocil (20% Paraquat + 10% Diurom) at doses: 1.5 (B4) and 2 L&middotha-1 (B5), and a control which received no application (B6). Seed viability was assessed through the germination standard test, and vigor through the first germination count, accelerated aging, electrical conductivity, seedling length, seedling dry matter and biomass density tests. The experimental design was the completely randomized, with four replications. Data were submitted to variance analysis, and when significant effects were observed, the Scott- Knott test was carried out at 5% probability using the SISVAR 5.1 software. The results showed that: a) the application of 1.875 L&middotha-1 of glyphosate was harmful to pigeonpea seed viability and vigor, evaluated through the accelerated aging test;b) applications of glyphosate in a 1.875 L&middotha-1 dose and Gramocil in 2 L&middotha-1 resulted in low vigor according to the electrical conductivity test of seeds;and c) the seed vigor measured by the first count, seedling length, seedling dry matter and biomass density test was not influenced by the type of desiccant applied. 展开更多
关键词 cajanus cajan(l.)Millsp. Pre-Harvest Desiccation Deterioration Maturity VIGOR
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