Epigenetic modification in histone deacetylase deletion strain of Calcarisporium arbuscula leads to diverse diterpenoids

Epigenetic modifications have been proved to be a powerful way to activate silent gene clusters and lead to diverse secondary metabolites in fungi. Previously, inactivation of a histone H3 deacetylase in Calcarisporium arbuscula had led to pleiotropic activation and overexpression of more than 75% of the biosynthetic genes and isolation of ten compounds. Further investigation of the crude extract of C. arbuscula Δhda A strain resulted in the isolation of twelve new diterpenoids including three cassanes(1-3), one cleistanthane(4), six pimaranes(5-10), and two isopimaranes(11 and 12) along with two know cleistanthane analogues. Their structures were elucidated by extensive NMR spectroscopic data analysis. Compounds 2 and 4 showed potent inhibitory effects on the expression of MMP1 and MMP2(matrix metalloproteinases family) in human breast cancer(MCF-7) cells.

灵芝一共生真菌的脑苷类成分研究

目的 为了从灵芝 Ganoderma lucid um中寻找新的天然抗肿瘤活性产物 ,对其次生代谢产物进行了较系统的研究。方法 利用柱层析和制备 RP-HPL C从灵芝一共生真菌 (Calcarisp orium arbuscula)的发酵菌丝体中分得 4个脑苷类化合物。结果 通过光谱分析鉴定 ,分别为 :(4 E,8E,3′E,2 S,3R,2′R) -2′-羟基 -3′-十六烯酰基 -1-O-D-吡喃葡萄糖基 -9-甲基 -4 ,8-二氢鞘氨二烯醇 ( ) ,(4 E,8E,2 S,3R,2′R) -2′-羟基十六烷酰基 -1-O-β-D-吡喃葡萄糖基 -9-甲基 -4 ,8-二氢鞘氨二烯醇 ( ) ,(4 E,8E,3′E,2 S,3R,2′R) -2′-羟基 -3′-十八烯酰基 -1-O-D-吡喃葡萄糖基 -9-甲基 -4 ,8-二氢鞘氨二烯醇 ( ) ,2 -(2 -羟基二十四烷酰氨基 ) -1,3 ,4 -十八烷三醇 ( )。结论 ～ 均首次从齿梗孢属真菌中分离得到。

赤芝一共生菌发酵过滤液中的新倍半萜酯(英文)

从四川野生赤芝 (Ganodermalucidum (Leyss.exFr.)Karst.)的一共生菌CalcarisporiumarbusculaPreuss的发酵过滤液中分离得到 3个新的倍半萜酯类化合物 ,分别命名为calcarisporinB2 (1)、B3 (2 )和B4(3) ,通过物理方法和波谱分析确定了它们的结构。

灵芝一共生真菌的发酵液化学成分研究

从灵芝一共生真菌 (CalcarisporiumarbusculaPreuss)的发酵液中分离得到 7个化合物 ,经波谱分析确定其结构分别为 :zythiostromicacidA(1) ,zythiostromolide(2 ) ,7,8 二甲基苯并 [g]蝶啶 2 ,4 (1H ,3H) 二酮 (7,8 dimethylbenzo[g]pteridine 2 ,4 (1H ,3H) dione ,3) ,麦角甾醇 (ergosterol,4 ) ,琥珀酸 (butane dioicacid ,5 ) ,狼毒甲素 (5 ,5’ [oxybis(methylene) ]bis 2 furancarboxaldehyde ,6 ) ,5 羟甲基糠醛 (5 hydrox ylmethyl 2 furancarboxaldehyde ,7)。 1,2 ,3首次从齿梗孢属真菌中分离得到。