<strong>Background:</strong> We aimed to investigate the ability of carbon nanoparticles to identify parathyroid glands with vasculature during thyroid surgery. <strong>Material and methods:</stro...<strong>Background:</strong> We aimed to investigate the ability of carbon nanoparticles to identify parathyroid glands with vasculature during thyroid surgery. <strong>Material and methods:</strong> Totally 42 patients with various thyroid diseases were selected for the prospective research of carbon nanoparticle injection used in thyroidectomy. Another 42 patients without receiving carbon nanoparticle injection were selected as the control group. All cases underwent total or subtotal bilateral thyroidectomy. Before the ligation of the superior and inferior poles of the thyriod lobes, 0.1 mL of a carbon nanoparticle suspension was injected into the two poles. Important tissues such as the recurrent laryngeal nerve, parathyroid gland with blood supply and the inferior thyroid artery were identified and protected. <strong>Results:</strong> The parathyroid glands with vasculature were not stained and thus remained the primary color in all cases, while abnormal thyroid tissues were stained black. After 5 minutes, thyroid lobes were injected with the carbon nanoparticle suspension and the original color of parathyroid glands was unchanged. Even the parathyroids with blood supply can be identified and protected. The number of parathyroid glands for autotransplantation was one in the test group and six in the control group. At one day after the thyroid surgery, hypocalcemia became detectable in four patients of the test group and in five of the control group. Twenty-four instances of hypoparathyroidism occurred at 1 day after surgery in the control group, while ten instances in the test group. <strong>Conclusion:</strong> Intraoperatvie carbon nanoparticle suspension injection is an effective and safe technique for guiding thyroid surgery. The carbon nanoparticle suspension plays an important role in identifying the vasculatural parathyroid glands, while protecting the physiologic function of the parathyroid glands during surgery.展开更多
The astonishingly distinct lubricity difference between the ionic liquid modified carbon nanoparticles grafted by anion and cation moieties(A-g-CNPs and C-g-CNPs)was well established as additives of polyethylene glyco...The astonishingly distinct lubricity difference between the ionic liquid modified carbon nanoparticles grafted by anion and cation moieties(A-g-CNPs and C-g-CNPs)was well established as additives of polyethylene glycol(PEG200).The peripheral anion moieties and positively charged inner parts of C-g-CNPs could successively absorb onto the friction interfaces by electrostatic interactions to form the organic-inorganic electric double layer structures,tremendously boosting the lubricity of PEG200.Contrarily,the preferentially electrostatic adsorption of negatively charged inner parts but repulsion of the peripheral cation moieties determined the weak embedded stability of A-g-CNPs between the friction interfaces,even impairing the lubricity of PEG200.This work can offer solidly experimental and theoretical guidance for designing and developing the high-performance nanoadditives modified by ionic molecules.展开更多
目的对照研究计算机辅助检测(computer-aided detection,CAD)自动提取的微钙化多种特征与乳腺导管原位癌(ductal carcinoma in situ,DCIS)不同病理分级的相关性,探讨所有特征对于DCIS不同分级的意义,为开发辅助临床早期诊断和个体化治疗...目的对照研究计算机辅助检测(computer-aided detection,CAD)自动提取的微钙化多种特征与乳腺导管原位癌(ductal carcinoma in situ,DCIS)不同病理分级的相关性,探讨所有特征对于DCIS不同分级的意义,为开发辅助临床早期诊断和个体化治疗DCIS的计算机辅助诊断系统提供参考。方法经本院乳腺钼靶X线摄影检查发现微钙化并经最终病理学证实的203例DCIS患者影像资料,按高、中、低级别病理结果分组,CAD检测微钙化并自动提取反映微钙化形态学、统计学、纹理、分布特征的14个参数,进行对比分析。结果 1所有微钙化特征中,线样分支状钙化数、细颗粒状微钙化率、总数目、簇状分布率、种群密度在低、中、高级别3组间比较和低、高级别2组间比较时差异均具有统计学意义(P<0.01),较高的病理级别容易出现较高的线样分支状钙化数、总数目、簇状分布率、种群密度,较低的病理级别出现细颗粒状微钙化的概率较高。2除线样分支状钙化数外,其余所有特征参数在中级别组和低级别或高级别组间比较时,差异均无统计学意义(P>0.05)。3微钙化的四种分布类型仅在高级别组出现统计学差异,其中簇状分布的出现概率相对较高。结论通过CAD辅助检测DCIS微钙化获得的多种特征参数,与不同生物学行为的病理分级相关性不同,有利于深入研究计算机辅助诊断DCIS,为早期诊断DCIS和临床个性化治疗提供重要的参考依据。展开更多
Purpose: Identifying the source of stone formation in recurrent stone formers has always been a big problem. Material and Methods: In this study kidney stones from 52 patients were submitted to direct examination by s...Purpose: Identifying the source of stone formation in recurrent stone formers has always been a big problem. Material and Methods: In this study kidney stones from 52 patients were submitted to direct examination by scanning electronic microscopy (SEM) after manual fracture and 27 calculi were cultured in Eagle’s Minimum Essential Medium (E-MEM) and Brain-Heart Infusion (BHI) for eight weeks at 37°C in 5% CO2 atmosphere. Twenty-seven powdered and demineralized stones were suspended in sterile PBS, filtered through 0.22 m-pore-size sterile filters Millex (Millipore, Massachusetts, USA) and submitted to DNA extraction (Quiagen-Brazil). Platinum PCR SuperMIX (GIBCO-BRL), primers (Invitrogen), and Ultra Pure Water (Advanced Biotechnologies, Columbia, MD) were used for PCR (Polymerase Chain Reaction), which was generally conducted for 30 or 35 cycles with annealing of primers for 40 sec at 55°C and extension for 1 min at 72°C. Results: In 36 out 52 (69%) kidney stones it was detected the presence of biofilm coating the mineral surface of the stone when examined by SEM, consisting of coccoid particles, isolated or clustered, with diameter of 500 nm or less. Eleven out 27 (41%) kidney stone cultures produced white-colored sediment on the bottom of the tubes after eight-week incubation, revealing tiny structures similar to those observed directly by SEM. These structures were similar in size and morphology to spherical apatite particles previously observed in human kidney stones and named as nanobacteria (NB). No PCR products were observed in the samples. Conclusion: We found a strong correlation between renal stones and calcifying nanoparticles (CNP) in this study and these results open a new insight on this area to explore the etiology of stone formation. Whether NB/CNP are truly microorganisms or self-propagating mineral compounds is still controversial and its contribution, if any, in apatite nucleation and crystal growth remains uncertain.展开更多
A simple （2×2） pixelated flexible infrared nanosensor array based on carbon nanoparticles （CNPs） was fabricated through a simple and low-cost flame method. By integrated with a micro controller unit, the sens...A simple （2×2） pixelated flexible infrared nanosensor array based on carbon nanoparticles （CNPs） was fabricated through a simple and low-cost flame method. By integrated with a micro controller unit, the sensor array could detect power density of incident infrared light in real-time. The mechanism for the superior infrared sensing property of the flexible sensor array based on CNP was also studied in detail in this work.展开更多
文摘<strong>Background:</strong> We aimed to investigate the ability of carbon nanoparticles to identify parathyroid glands with vasculature during thyroid surgery. <strong>Material and methods:</strong> Totally 42 patients with various thyroid diseases were selected for the prospective research of carbon nanoparticle injection used in thyroidectomy. Another 42 patients without receiving carbon nanoparticle injection were selected as the control group. All cases underwent total or subtotal bilateral thyroidectomy. Before the ligation of the superior and inferior poles of the thyriod lobes, 0.1 mL of a carbon nanoparticle suspension was injected into the two poles. Important tissues such as the recurrent laryngeal nerve, parathyroid gland with blood supply and the inferior thyroid artery were identified and protected. <strong>Results:</strong> The parathyroid glands with vasculature were not stained and thus remained the primary color in all cases, while abnormal thyroid tissues were stained black. After 5 minutes, thyroid lobes were injected with the carbon nanoparticle suspension and the original color of parathyroid glands was unchanged. Even the parathyroids with blood supply can be identified and protected. The number of parathyroid glands for autotransplantation was one in the test group and six in the control group. At one day after the thyroid surgery, hypocalcemia became detectable in four patients of the test group and in five of the control group. Twenty-four instances of hypoparathyroidism occurred at 1 day after surgery in the control group, while ten instances in the test group. <strong>Conclusion:</strong> Intraoperatvie carbon nanoparticle suspension injection is an effective and safe technique for guiding thyroid surgery. The carbon nanoparticle suspension plays an important role in identifying the vasculatural parathyroid glands, while protecting the physiologic function of the parathyroid glands during surgery.
基金financially supported by the National Natural Science Foundation of China(No.51975493).
文摘The astonishingly distinct lubricity difference between the ionic liquid modified carbon nanoparticles grafted by anion and cation moieties(A-g-CNPs and C-g-CNPs)was well established as additives of polyethylene glycol(PEG200).The peripheral anion moieties and positively charged inner parts of C-g-CNPs could successively absorb onto the friction interfaces by electrostatic interactions to form the organic-inorganic electric double layer structures,tremendously boosting the lubricity of PEG200.Contrarily,the preferentially electrostatic adsorption of negatively charged inner parts but repulsion of the peripheral cation moieties determined the weak embedded stability of A-g-CNPs between the friction interfaces,even impairing the lubricity of PEG200.This work can offer solidly experimental and theoretical guidance for designing and developing the high-performance nanoadditives modified by ionic molecules.
文摘Purpose: Identifying the source of stone formation in recurrent stone formers has always been a big problem. Material and Methods: In this study kidney stones from 52 patients were submitted to direct examination by scanning electronic microscopy (SEM) after manual fracture and 27 calculi were cultured in Eagle’s Minimum Essential Medium (E-MEM) and Brain-Heart Infusion (BHI) for eight weeks at 37°C in 5% CO2 atmosphere. Twenty-seven powdered and demineralized stones were suspended in sterile PBS, filtered through 0.22 m-pore-size sterile filters Millex (Millipore, Massachusetts, USA) and submitted to DNA extraction (Quiagen-Brazil). Platinum PCR SuperMIX (GIBCO-BRL), primers (Invitrogen), and Ultra Pure Water (Advanced Biotechnologies, Columbia, MD) were used for PCR (Polymerase Chain Reaction), which was generally conducted for 30 or 35 cycles with annealing of primers for 40 sec at 55°C and extension for 1 min at 72°C. Results: In 36 out 52 (69%) kidney stones it was detected the presence of biofilm coating the mineral surface of the stone when examined by SEM, consisting of coccoid particles, isolated or clustered, with diameter of 500 nm or less. Eleven out 27 (41%) kidney stone cultures produced white-colored sediment on the bottom of the tubes after eight-week incubation, revealing tiny structures similar to those observed directly by SEM. These structures were similar in size and morphology to spherical apatite particles previously observed in human kidney stones and named as nanobacteria (NB). No PCR products were observed in the samples. Conclusion: We found a strong correlation between renal stones and calcifying nanoparticles (CNP) in this study and these results open a new insight on this area to explore the etiology of stone formation. Whether NB/CNP are truly microorganisms or self-propagating mineral compounds is still controversial and its contribution, if any, in apatite nucleation and crystal growth remains uncertain.
文摘A simple （2×2） pixelated flexible infrared nanosensor array based on carbon nanoparticles （CNPs） was fabricated through a simple and low-cost flame method. By integrated with a micro controller unit, the sensor array could detect power density of incident infrared light in real-time. The mechanism for the superior infrared sensing property of the flexible sensor array based on CNP was also studied in detail in this work.
