南海海绵Callyspongia fallax化学成分的研究

对南海海绵Callyspongia fallax进行次级代谢产物的研究。采用多种色谱分离技术对95%乙醇粗提物进行分离纯化,利用核磁共振(NMR)和质谱(MS)等波谱学手段,与报道的数据进行对比,鉴定得到7个化合物,分别是(2S,3R,4E)-N-palmitoyl-octadecasphinga-4-ene(1);3,6-二苯甲基哌嗪-2,5-二酮(2);β-胆甾醇(3);胸腺嘧啶(4);尿嘧啶核苷(5);脱氧尿嘧啶核苷(6);脱氧胸腺嘧啶核苷(7)。对得到的化合物采用MTT法评价其细胞毒活性,化合物1-7均没有表现出明显细胞毒活性。化合物1和2是首次从该种属海绵中分离得到,该工作的开展丰富了美丽属海绵化合物种类多样性。

海绵Callyspongia Fibrosa的化学成分研究(Ⅰ)

从中国南海海绵中分离鉴定出 5个化合物 ,其结构确定为对羟基苯甲酸 ( Ⅰ)、鲨肝醇 ( Ⅱ )、胸腺嘧啶( Ⅲ )、尿嘧啶 ( Ⅳ )、胸腺嘧啶脱氧核苷 (Ⅴ )。

Isolation and molecular characterization of bioactive secondary metabolites from Callyspongia spp.associated fungi

Objective:To isolate and characterize the bioactive secondary metabolite from Callyspongia spp.associated fungi.Methods:In vitro antibacterial screening of fungi associated with Callyspongia species,collected from south east coast of India,against selected clinical isolates of bacteria were conducted in this study.The extracts showing good antimicrobial activity were subjected to further analysis to identify the active constituents sponge associated fungi(both biomass and filtrate) with five different solvents.The compound responsible for bioactivity was characterized using Fouvier-transform infrared(FT-IR) and gas chromatography-mass spectrometry(GC-MS) instrumental analysis to identify the functional group and compound.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(ITS) region of 5.8srRNA using specific ITS primer. The novelty of the strain was proved by BlastN analysis against non-redundant(NR) database and hence was submitted to GenBank.Results:Active compound was Desmethylnomifensine confirmed by GC-MS and the potent fungi was Aspergillus flavus GU815344.Conclusions: The isolate exhibits a marked antagonistic activity against potential bacterial pathogens thus illuminating the advanced researches in this decade to focus on clinical pharmacology to identify novel therapeutic targets.The present study depicts a promising scenario to focus on Aspergillus flavus derived compounds which can be easily scaled up for large biomass production and stable formulation as a drug.

Essential metal Zn in sponge <i>Callyspongia aerizusa</i>from Spermonde Archipelago

Spermonde Archipelago has been found to have a high sponge biodiversity. Sponges that have feeding behavior as filter feeder can be exposed to heavy metals, including Zn metal. Zn is an essential metal that is physiologically needed by sponge to catalyze chemical reactions and generally operates on enzymes. A study on Zn pollution level in Spermonde Archipelago from zone I to Zone IV was conducted by analyzing Zn concentration in sponges, sediments, and seawater. Physicochemical parameters measurement in Spermonde Archipelago indicates that Spermonde Archipelago meets the requirements for sponge growth. Callyspongia aerizusa can be used as metal pollution indicator because this sponge can accumulate heavy metal Zn in high level (99.30 mg/kg dry weight). This sponge was derived from Kondingareng Lompo island at zone III. In addition, Kodingareng Lompo island waters had the highest Zn concentration compared to other islands. Sediments from Lae-Lae island at zone I had the highest Zn metal concentration. Lae-Lae island is the nearest island to Makassar coastal line.

中国广西涠洲岛海域海绵Callyspongia sp.的化学成分研究

目的:对采自中国广西涠洲岛海域海绵Callyspongia sp.的化学成分进行分离和结构鉴定。方法:采用硅胶薄层色谱、柱色谱、凝胶渗透色谱LH-20,半制备HPLC等方法对采自中国广西涠洲岛海域海绵Callyspongia sp.的次生代谢产物进行化学分离,并通过核磁共振谱(1D、2D NMR)、质谱(ESI-MS)等方法,确定化合物的结构。结果:共分离得到8个化合物,分别为牛磺酸(1)、乙酰牛磺酸(2)、N-甲基吡啶-2-羧酸盐(3)、zooanemonin(4)、N-丁基乙酰胺(5)、尿嘧啶(6)、3-甲基尿嘧啶(7)、苯甲酸(8)。结论:化合物1-5和7-8均为首次从该属海绵中分离得到。

美丽属海绵次生代谢产物及其生物活性

美丽属海绵Callyspongia sp.分布广泛,含有多种生物活性成分,如多炔、肽类、生物碱、脂肪酸、聚酮类、甾体等,其中许多化合物具有抗污浊、抗肿瘤和抗菌等多种生物活性。按化合物的结构类型简要介绍了近几十年来美丽属海绵化学成分及其生物活性的研究进展,为进一步的研究工作提供参考。

A preliminary effort to assign sponge (<i>Callispongia sp</i>) as trace metal biomonitor for Pb, Cd, Zn, and Cr, an environmental perspective in Hative gulf waters Ambon

The aim of this investigation is to estimate metal concentration distribution, Pb, Cd, Zn, and Cr, in Callispongia sp, sediment, and water column in Hative waters of Ambon bay. After sampling, analytical processes were conducted by using a standard method and measured by ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy). The results showed that Zn concentration was highest, 0.231 mg/kg dry weight (ppm) while in sediment and water the numbers were 1.180 and 0.790 ppm respectively. In the meantime, functional group studies through Fourier Transformed Infra Red spectroscopy revealed that OH-group (3421 cm-1), Conjugated Double Bond (1635 cm-1) and Ether Groups (1049 cm-1) of callispongia sp were found. Also, the appearance of wave-number 2926 cm-1 clearly indicates the existence of –CH sp3 group. Combining both results may guide us to establish a relation between metal concentration and organic substance transformation made during interaction inside species. Thus, trace metal investigation can be a choice in doing rapid assessment and biomonitor for the quality of coastal marine life. Furthermore, by more extensive studies, this connection could be developed and proposed as a low cost method for EIA of metal pollution in coastal zone, particularly in coral reef system.

南海美丽海绵属化学成分研究

目的研究南海美丽海绵属Callyspongia sp.的化学成分。方法采用硅胶柱色谱、Sephadex LH-20和反相半制备HPLC等方法分离纯化,根据现代波谱技术（ESI-MS,^1H-NMR,^13C-NMR）进行结构鉴定。结果从南海美丽海绵属Cally-spongiasp.分离得到8个化合物,分别鉴定为：尿嘧啶（1）、胸腺嘧啶（2）、β-吲哚醛（3）、4-羟基苯乙酰胺（4）、3,4-二氯苯甲酸（5）、腺嘌呤（6）、苯氨基丙酰胺（7）、4-（2-氨乙基）-N,N-二乙苯胺（8）。结论其中化合物2-8为首次从南海美丽海绵属Callyspongiasp.中分离得到。