11份毛蔓豆属种质营养价值评价

本研究对11份毛蔓豆属种质的常规营养成分进行测定分析,并采用主成分、隶属函数值法和聚类分析对其营养价值进行综合评价。结果表明:11份毛蔓豆种质的粗蛋白、粗灰分、中性洗涤纤维、酸性洗涤纤维、钙、磷含量间差异极显著,将其隶属函数值进行聚类,分为2类,其中,041130150毛蔓豆、051210057毛蔓豆、050320021毛蔓豆、050106023毛蔓豆、050319007毛蔓豆为高营养价值种质,其他种质综合营养价值较低;通过主成分分析发现,磷、中性洗涤纤维和粗蛋白的含量可作为其营养价值评价的重要指标。

Germination and Vigor of Fodder Fabacceae Seeds Submitted to in Vitro and in Situ Incubation

The current study was developed with the purpose to evaluate the germination and the vigor of Kudzu (Pueraria phaseoloides), Leucaena (Leucaena leucocephala) and Calopog?nio seeds (Calopogonium mucunoides) submitted to 0, 6, 12, 24, 48, 72 and 96 hours of testing to natural (in situ incubation) and simulated conditions (in vitro incubation) from the ruminal environment of bovines. For each period of in situ and in vitro incubation, in each kind of seed, the percentage of normal plants was evaluated, as well as abnormal plants, hard seeds, soaked seeds and dead seeds, besides the index of germination speed (IGS). The results were submitted to the Duncan test at a 5% probability. Higher percentages of normal plants were verified in the Kudzu and in the Leucaena kinds, when the seeds were submitted to in situ incubation, as well as the IGS for the three forage species. The Calopog?nio seeds did not turn out to be susceptible of use in the in situ and in vitro incubations. The in vitro incubation revealed to be more harmful to the seeds of the three species used.

Germination,Vigor of Seeds and Emergence of Fabaceae Seedling in Bovine Faeces

This study was conducted to evaluate the physiological quality of forage seeds after passage through the gastrointestinal tract of cattle, as well as the emergence of seedlings derived from these seeds. Three Fabaceae species were used: tropical kudzu, leucaena and calopo. One hundred grams of seeds of each species were offered, separately, to five bovines. After the start of the dispersion, the faeces of the animals were collected every 6 hours, in a total of 72 hours, being taken to the greenhouse immediately after each collection. The study evaluated the total number of seedlings in the stool, the lengths of roots and aerial parts, as well as the natural and dry seedling weight thirty days after the disposal of the faeces in the greenhouse. The experimental design consisted of randomized blocks, with twelve treatments (sampling periods) and five replications (cattle). The best performance for the total number of seedlings in the faeces of cattle was observed in the kudzu species. The length of the root, length of the aerial part and dry matter seedling weight was observed in the leucaena species, followed by kudzu. The calopo showed low results for all variables due to the high degradation of the seeds.

Antimicrobial activity and DNA-fragmentation effect of isoflavonoids isolated from seeds of Millettia ferruginea,an endemic legume tree in Ethiopia

Objective:To investigate the seeds of Millettia ferruginea(M.ferruginea)to unravel its antibacterial,antifungal,antitubercular,and antileishmanial potential for the first time.Methods:M.ferruginea seeds were refluxed separately with chloroform,methanol and water to prepare the three extracts,which were tested against the reference strains of Gram-positive and Gram-negative bacteria,yeast cells,Mycobacterium tuberculosis and Leishmania donovani(L.donovani)promastigotes.Next,the seeds were chemically analysed to isolate three constituent compounds,viz.,barbigerone,calopogonium isoflavone-A and durmillone,which were purified,characterised and evaluated for antibacterial and antileishmanial activity.Further,Comet assay was conducted to observe DNA fragmentation effects on human peripheral blood mononuclear cells pretreated with the isoflavonoid compounds.Results:The chloroform and methanol extracts of M.ferruginea seeds exhibited antibacterial and antileishmanial activity.The pure compounds also showed inhibitory activity against Gram-negative ATCC strains(minimum inhibitory concentration~0.5μmol/L),and L.donovani promastigotes(IC_(50)8.2-87.3μg/mL).However,they had little or no activity against yeast cells and tubercle bacilli.The DNA fragmentation study showed that the isoflavonoid constituents of M.ferruginea seeds were safe at therapeutic doses.Conclusions:The antibacterial efficacy of the non-aqueous extracts of M.ferruginea seed was observed against both Gram-positive and Gram-negative ATCC strains.Moreover,the constituents isoflavonoids,viz.,barbigerone,calopogonium isoflavone-A and durmillone,exhibited inhibitory activity against Gram-negative ATCC strains and L.donovani promastigotes.The comet assay showed that the compounds were safe to be considered for human consumption.