Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. W...Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.展开更多
Background:The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation.Methods:Bone marrow mesenchymal cells were characterized for ost...Background:The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation.Methods:Bone marrow mesenchymal cells were characterized for osteoblastic phenotype.Calvariae of male Wistar rats were irradiated(20 Gy)and,after 4 weeks,osteoblastic cells were placed in surgically created defects in irradiated(IRC)and control animals(CC),paired with untreated irradiated(IR)and control(C)animals.After 30 days,histological and microtomographic evaluation was performed to establish significant(P<0.05)differences among the groups.Results:Higher alkaline phosphatase detection and activity,along with an increase in mineralized nodules,in the IRC,C and CC groups compared to the IR group,confirmed an osteoblastic phenotype.Histology showed impaired bone neoformation following irradiation,affecting bone marrow composition.Cell therapy in the IRC group improved bone neoformation compared to the IR group.Microtomography revealed increased bone volume,bone surface and trabecular number in IRC group compared to the IR group.Conclusion:Cell therapy may improve bone neoformation in defects created after irradiation.展开更多
为研究细胞毒性物质对成骨细胞的影响,采用 24 h 内乳鼠头盖骨,对国外现行报道方法进行了改进,建立了适合我国同类试验室条件下的骨组织细胞培养方法——酶消化分离培养法,并用该新方法培养出了具有典型特征的成骨细胞系。研究结果表明...为研究细胞毒性物质对成骨细胞的影响,采用 24 h 内乳鼠头盖骨,对国外现行报道方法进行了改进,建立了适合我国同类试验室条件下的骨组织细胞培养方法——酶消化分离培养法,并用该新方法培养出了具有典型特征的成骨细胞系。研究结果表明,此法具有耗时短、消化充分和对细胞损伤小的特点,能够满足试验的要求。镜下细胞形态不规则,多呈三角形、多角形,有突起,胞质丰富、清晰。应用特异性染色方法,胞质中呈现相应的特征性染色。培养过程中,成骨细胞经历静止期、指数成长期和滞后期等三个阶段。培养 14 d 后,出现矿化结节。展开更多
文摘Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.
基金National Institute of Science and Technology, Translational Medicine, Brazil (INCT.TM)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
文摘Background:The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation.Methods:Bone marrow mesenchymal cells were characterized for osteoblastic phenotype.Calvariae of male Wistar rats were irradiated(20 Gy)and,after 4 weeks,osteoblastic cells were placed in surgically created defects in irradiated(IRC)and control animals(CC),paired with untreated irradiated(IR)and control(C)animals.After 30 days,histological and microtomographic evaluation was performed to establish significant(P<0.05)differences among the groups.Results:Higher alkaline phosphatase detection and activity,along with an increase in mineralized nodules,in the IRC,C and CC groups compared to the IR group,confirmed an osteoblastic phenotype.Histology showed impaired bone neoformation following irradiation,affecting bone marrow composition.Cell therapy in the IRC group improved bone neoformation compared to the IR group.Microtomography revealed increased bone volume,bone surface and trabecular number in IRC group compared to the IR group.Conclusion:Cell therapy may improve bone neoformation in defects created after irradiation.
文摘为研究细胞毒性物质对成骨细胞的影响,采用 24 h 内乳鼠头盖骨,对国外现行报道方法进行了改进,建立了适合我国同类试验室条件下的骨组织细胞培养方法——酶消化分离培养法,并用该新方法培养出了具有典型特征的成骨细胞系。研究结果表明,此法具有耗时短、消化充分和对细胞损伤小的特点,能够满足试验的要求。镜下细胞形态不规则,多呈三角形、多角形,有突起,胞质丰富、清晰。应用特异性染色方法,胞质中呈现相应的特征性染色。培养过程中,成骨细胞经历静止期、指数成长期和滞后期等三个阶段。培养 14 d 后,出现矿化结节。
