Graphene oxide influences bacterial community and soil environments of Cd-polluted Haplic Cambisols in Northeast China

Graphene oxide(GO),a carbon nanomaterial that is widely used in the environment and other industries,may pose potential risks to ecosystems,especially the soil ecosystem.Some soils in Northeast China are frequently polluted with cadmium(Cd) metal.However,there is no study on the influence of GO on the Cd-contaminated soil microbial community and soil chemical properties.In this study,Cd(100 mg kg^(-1))-polluted soils were treated with different concentrations of GO(0,25,50,150,250,and 500 mg L^(-1),expressed as T1,T2,T3,T4,T5,and T6,respectively) for 40 days.The treatment without Cd pollution and GO served as the control(CK).Then,we investigated the influence of the GO concentrations on the bacterial community and chemical properties of Cd-polluted Haplic Cambisols,the zonal soil in Northeast China.After GO addition,the richness and diversity indexes of the bacterial community in Cd-contaminated Haplic Cambisols initially increased by 0.05-33.92% at 25 mg L^(-1),then decreased by0.07-2.37% at 50 mg L^(-1),and then increased by 0.01-24.37%within 500 mg L^(-1) again.The species and abundance of bacteria varied with GO concentration,and GO significantly increased bacterial growth at 25 and 250 mg L^(-1).GO treatments influenced the bacterial community structure,and the order of similarity of the bacterial community structure was as follows:T4=T5> T1=T6> T2> T3> CK.Proteobacteria and Acidobacteria were the dominant bacteria,accounting for 36.0% and 26.2%,respectively,of soil bacteria.Different GO treatments also significantly affected the metabolic function of bacteria and further influenced the diversity of the bacterial community structure by affecting several key soil chemical properties:soil pH,organic matter and available potassium,phosphorus,and cadmium.Our results provide a theoretical basis for scientific and comprehensive evaluation of the environmental impacts of GO on the zonal forest soils of Northeast China.

Corn straw return can increase labile soil organic carbon fractions and improve water-stable aggregates in Haplic Cambisol

Corn straw return to the field is a vital agronomic practice for increasing soil organic carbon(SOC)and its labile fractions,as well as soil aggregates and organic carbon(OC)associated with water-stable aggregates(WSA).Moreover,the labile SOC fractions play an important role in OC turnover and sequestration.The aims of this study were to determine how different corn straw returning modes affect the contents of labile SOC fractions and OC associated with WSA.Corn straw was returned in the following depths:(1)on undisturbed soil surface(NTS),(2)in the 0–10 cm soil depth(MTS),(3)in the 0–20 cm soil depth(CTS),and(4)no corn straw applied(CK).After five years(2014–2018),soil was sampled in the 0–20 and 20–40 cm depths to measure the water-extractable organic C(WEOC),permanganate oxidizable C(KMnO4-C),light fraction organic C(LFOC),and WSA fractions.The results showed that compared with CK,corn straw amended soils(NTS,MTS and CTS)increased SOC content by 11.55%–16.58%,WEOC by 41.38%–51.42%,KMnO4-C and LFOC by 29.84%–34.09%and 56.68%–65.36%in the 0–40 cm soil depth.The LFOC and KMnO4-C were proved to be the most sensitive fractions to different corn straw returning modes.Compared with CK,soils amended with corn straw increased mean weight diameter by 24.24%–40.48%in the 0–20 cm soil depth.The NTS and MTS preserved more than 60.00%of OC in macro-aggregates compared with CK.No significant difference was found in corn yield across all corn straw returning modes throughout the study period,indicating that adoption of NTS and MTS would increase SOC content and improve soil structure,and would not decline crop production.

Study on Soil Denitrification in Wheat-Maize Rotation System

Soil denitrification was studied in wheat-maize rotation cropping system on an aquic cambisol. Results showed that the N loss amount by denitrification ranged from 4.7 to 9.7 kg per hectare with different levels of nitrogen application and the key stage for denitification was during summer maize-growth-period, especially within 1-2 weeks after fertilizer nitrogen was applied. Similar trend was found between soil N2O production/emission dynamic and denitrification dynamic in the rotation system, which may indicate that mainly N2O is produced in nitrification process.