Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific...Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific genetic diversity in tick populations. In this study, the genetic diversity of four H. dromedarii populations was investigated using mitochondrial cytochrome c oxidase subunit I (COI) gene and randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). The results showed that both of the aforementioned techniques produced similar grouping patterns. Moreover, they revealed that the four tick populations had high levels of genetic similarity. However, one population was slightly different from the three other populations. The current study demonstrated that H. dromedarii ticks in the UAE are very similar at the genetic level and that investigating more locations and screening larger numbers of ticks could reveal larger genetic differences.展开更多
为了解巴里坤县双峰驼体表蜱的种类及其携带病原,采集了巴里坤县双峰驼体表寄生蜱样本104只,在形态学分类的基础上,基于蜱的线粒体16S rDNA基因、梨形虫18S r DNA基因和立克次体16S rDNA基因,对48只蜱的DNA样本进行PCR扩增。结果显示,...为了解巴里坤县双峰驼体表蜱的种类及其携带病原,采集了巴里坤县双峰驼体表寄生蜱样本104只,在形态学分类的基础上,基于蜱的线粒体16S rDNA基因、梨形虫18S r DNA基因和立克次体16S rDNA基因,对48只蜱的DNA样本进行PCR扩增。结果显示,经形态学鉴定,104只蜱均为亚洲璃眼蜱;48个蜱DNA样本经PCR扩增和测序,均鉴定为亚洲璃眼蜱,存在9个基因型,以基因型1(n=24)、基因型2(n=4)和基因型3(n=12)为主要感染基因型,基因型4有2个样本,基因型5~9均有1个样本。48个蜱DNA样本检测出犬埃里克体1份,未发现梨形虫感染。结果表明,巴里坤县双峰驼体表蜱以亚洲璃眼蜱为优势种,存在遗传多样性,并发现亚洲璃眼蜱可携带犬埃里克体。展开更多
文摘Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific genetic diversity in tick populations. In this study, the genetic diversity of four H. dromedarii populations was investigated using mitochondrial cytochrome c oxidase subunit I (COI) gene and randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). The results showed that both of the aforementioned techniques produced similar grouping patterns. Moreover, they revealed that the four tick populations had high levels of genetic similarity. However, one population was slightly different from the three other populations. The current study demonstrated that H. dromedarii ticks in the UAE are very similar at the genetic level and that investigating more locations and screening larger numbers of ticks could reveal larger genetic differences.
文摘为了解巴里坤县双峰驼体表蜱的种类及其携带病原,采集了巴里坤县双峰驼体表寄生蜱样本104只,在形态学分类的基础上,基于蜱的线粒体16S rDNA基因、梨形虫18S r DNA基因和立克次体16S rDNA基因,对48只蜱的DNA样本进行PCR扩增。结果显示,经形态学鉴定,104只蜱均为亚洲璃眼蜱;48个蜱DNA样本经PCR扩增和测序,均鉴定为亚洲璃眼蜱,存在9个基因型,以基因型1(n=24)、基因型2(n=4)和基因型3(n=12)为主要感染基因型,基因型4有2个样本,基因型5~9均有1个样本。48个蜱DNA样本检测出犬埃里克体1份,未发现梨形虫感染。结果表明,巴里坤县双峰驼体表蜱以亚洲璃眼蜱为优势种,存在遗传多样性,并发现亚洲璃眼蜱可携带犬埃里克体。
