The genetic diversity analysis was done on 11 phenotypic traits of 35 camellia germplasm materials. The results showed that there were varying degrees of variation in 11 traits concerning the flowers and leaves select...The genetic diversity analysis was done on 11 phenotypic traits of 35 camellia germplasm materials. The results showed that there were varying degrees of variation in 11 traits concerning the flowers and leaves selected in this study,and petal number had the largest coefficient of variation,followed by ovary height,and leaf length had the smallest coefficient of variation. F test results showed that the differences in 11 phenotypic traits reached a significant level. Through the trait correlation analysis,it was found that there was a positive or negative significant correlation between the 11 phenotypic traits. Principal component analysis results showed that the cumulative contribution rate of the first three principal components was 71. 185%,the eigenvector which reflected flower pattern was largest,and flower pattern had a great impact on classification of camellia variety. Based on the genetic differences in traits between varieties,the 35 test materials were divided into three categories: Group I was characterized by large flower pattern; Group II was characterized by large leaf pattern; Group Ⅲ was characterized by many petals. Flower diameter and flower height were important standards for classification of camellia variety.展开更多
Discrimination of 24 wild tea germplasm resources (Camellia sp.) using RAPD markers was conducted. The result showed that RAPD markers were very effective tool and method in wild tea germplasm discrimination. There we...Discrimination of 24 wild tea germplasm resources (Camellia sp.) using RAPD markers was conducted. The result showed that RAPD markers were very effective tool and method in wild tea germplasm discrimination. There were 3 independent ways to discriminate tea germplasms, a) unique RAPD markers, b) specific band patterns and c) a combination of the band patterns or DNA fingerprinting provided by different primers. The presence of 16 unique RAPD markers and the absence of 3 unique markers obtained from 12 primers made it possible to discriminate 14 germplasms. Using the unique band patterns of primer OPO-13 could discriminate 10 tea germplasms. It was of much importance using minimum primers to obtain maximum discrimination capacity. All the 24 wild tea germplasms could be discriminated easily and entirely by the band patterns combination or DNA fingerprinting obtained from OPO-13, OPO-18, OPG-12 and OPA-13, including two wild tea trees of very similar morphological characteristics and chemical components.展开更多
This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also...This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also conducted to measure the major agronomic characteristics of 300 local germplasms.These results allowed us to select some superior lines as genetic resources for green-tea breeding and research genotypes.展开更多
文摘The genetic diversity analysis was done on 11 phenotypic traits of 35 camellia germplasm materials. The results showed that there were varying degrees of variation in 11 traits concerning the flowers and leaves selected in this study,and petal number had the largest coefficient of variation,followed by ovary height,and leaf length had the smallest coefficient of variation. F test results showed that the differences in 11 phenotypic traits reached a significant level. Through the trait correlation analysis,it was found that there was a positive or negative significant correlation between the 11 phenotypic traits. Principal component analysis results showed that the cumulative contribution rate of the first three principal components was 71. 185%,the eigenvector which reflected flower pattern was largest,and flower pattern had a great impact on classification of camellia variety. Based on the genetic differences in traits between varieties,the 35 test materials were divided into three categories: Group I was characterized by large flower pattern; Group II was characterized by large leaf pattern; Group Ⅲ was characterized by many petals. Flower diameter and flower height were important standards for classification of camellia variety.
基金Zhejiang Provincial New Century 151 Personnel Engineering ProgramChina and partially finished in the Centerfor Gene ResearchEhime University,Japan.
文摘Discrimination of 24 wild tea germplasm resources (Camellia sp.) using RAPD markers was conducted. The result showed that RAPD markers were very effective tool and method in wild tea germplasm discrimination. There were 3 independent ways to discriminate tea germplasms, a) unique RAPD markers, b) specific band patterns and c) a combination of the band patterns or DNA fingerprinting provided by different primers. The presence of 16 unique RAPD markers and the absence of 3 unique markers obtained from 12 primers made it possible to discriminate 14 germplasms. Using the unique band patterns of primer OPO-13 could discriminate 10 tea germplasms. It was of much importance using minimum primers to obtain maximum discrimination capacity. All the 24 wild tea germplasms could be discriminated easily and entirely by the band patterns combination or DNA fingerprinting obtained from OPO-13, OPO-18, OPG-12 and OPA-13, including two wild tea trees of very similar morphological characteristics and chemical components.
文摘This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also conducted to measure the major agronomic characteristics of 300 local germplasms.These results allowed us to select some superior lines as genetic resources for green-tea breeding and research genotypes.
