Valorization of Camellia oleifera oil processing byproducts to value-added chemicals and biobased materials: A critical review

The C.oleifera oil processing industry generates large amounts of solid wastes,including C.oleifera shell(COS)and C.oleifera cake(COC).Distinct from generally acknowledged lignocellulosic biomass(corn stover,bamboo,birch,etc.),Camellia wastes contain diverse bioactive substances in addition to the abundant lignocellulosic components,and thus,the biorefinery utilization of C.oleifera processing byproducts involves complicated processing technologies.This reviewfirst summarizes various technologies for extracting and converting the main components in C.oleifera oil processing byproducts into value-added chemicals and biobased materials,as well as their potential applications.Microwave,ultrasound,and Soxhlet extractions are compared for the extraction of functional bioactive components(tannin,flavonoid,saponin,etc.),while solvothermal conversion and pyrolysis are discussed for the conversion of lignocellulosic components into value-added chemicals.The application areas of these chemicals according to their properties are introduced in detail,including utilizing antioxidant and anti-in-flammatory properties of the bioactive substances for the specific application,as well as drop-in chemicals for the substitution of unrenewable fossil fuel-derived products.In addition to chemical production,biochar fabricated from COS and its applications in thefields of adsorption,supercapacitor,soil remediation and wood composites are comprehensively reviewed and discussed.Finally,based on the compositions and structural characteristics of C.oleifera byproducts,the development of full-component valorization strategies and the expansion of the appli-cationfields are proposed.

High temperature treatment induced production of unreduced 2n pollen in Camellia oleifera

Unreduced gametes through chromosome doubling play a major role in the process of plant polyploidization.Our previous work confirmed that Camellia oleifera can produce natural 2n pollen,and it is possible to induce the 2n pollen formation by high temperature treatment.This study focused on the optimization of the 2n pollen induction technique and the mechanisms of high temperature-induced2n pollen formation in C.oleifera.We found that the optimal protocol for inducing 2n pollen via high temperature was to perform 45℃with4 h at the prophaseⅠstage of the pollen mother cells(PMCs).Meanwhile,high temperature significantly decreased the yield and fertility of2n pollen.Through the observation of meiosis,abnormal chromosome and cytological behaviour was discovered under high-temperature treatment,and we confirmed that the formation of 2n pollen is caused by abnormal cell plate.Based on weighted gene co-expression network analysis,fifteen hub genes related to cell cycle control were identified.After male flower buds were exposed to heat shock,polygalacturonase gene(CoPGX3)was significantly upregulated.We inferred that high temperature causes the CoPGX3 gene to be overexpressed and that CoPGX3 is redistributed into the cytosol where it degrades cytoplasmic pectin,which leads to an abnormal cell plate.Furthermore,abnormal cytokinesis resulted in the formation of dyads and triads,and PMCs divided to produce 2n pollen.Our findings provide new insights into the mechanism of 2n pollen induced by high temperature in a woody plant and lay a foundation for further ploidy breeding of C.oleifera.

Synthesis and Characterization of Phenyl Camellia oleifera Seed Oil Ester Plasticizing PVC

Plasticizers are essential additives in the processing of polyvinyl chloride(PVC),with phthalate plasticizers being widely used.However,these conventional plasticizers have been shown to be harmful to human health and environmentally unfriendly,necessitating the exploration of eco-friendly bio-based alternatives.In this study,Camellia oleifera seed oil,a specialty resource in China,was utilized as a raw material and reacted with 4,4′-Methylenebis(N,N-diglycidylaniline)(AG-80)to synthesize Phenyl Camellia seed Oil Ester(PCSOE).PCSOE was employed as a plasticizer to prepare modified PVC films with varying concentrations,with the conventional plasticizer dioctyl phthalate(DOP)serving as a control.Experimental results demonstrate that PSCOE-plasticized PVC films exhibit enhanced hydrophilicity,tensile strength,and thermal stability compared to DOP-modified PVC films.The contact angle of PSCOE-plasticized PVC films ranges from 66.26°to 78.48°,which is generally lower than the contact angle of DOP-modified PVC films at 78.40°,indicating improved hydrophilicity due to the modification with PCSOE.The tensile strength of PSCOE-plasticized PVC films ranges from 17.73 to 20.17 MPa,all surpassing the value of 16.41 MPa for DOP-modified PVC films.Moreover,the temperatures corresponding to 5%,10%,and 50%weight loss for PVC samples modified with PCSOE are higher than those for DOP.Hence,PCSOE presents a viable alternative to DOP as a plasticizer for PVC materials.

Effects of Fungi Fusarium sp. to Rhizosphere Soil and Physiological Characteristics of Camellia oleifera Abel.

[Objectives]To study the effects of fungi Fusarium sp.to rhizosphere soil and physiological characteristics of Camellia oleifera Abel.[Methods]We investigated the effects of Fusarium sp.to rhizosphere soil nutrient element content and metabolites of C.oleifera.C.oleifera was inoculated with the suspension of Fusarium sp.in pot experiments and ammonium-N,available phosphorus,available potassi-um,organic matter,enzymes and pH of rhizosphere soil,MDA content,activity of SOD,POD of C.oleifera leaves were analyzed.[Results]Fusarium sp.stress significantly inhibited soil enzyme activities and significantly reduced available phosphorus content,especially for phospha-tase and sucrase.Antioxidant enzyme activities in C.oleifera tissues showed that Fusarium sp.stress significantly increased MDA and SOD enzyme activities and decreased POD enzyme activity.Especially,SOD enzyme activity was elevated by 53.86%compared with the CK group.In addition,analysis of the content of major metabolites in C.oleifera leaves showed that Fusarium sp.stress significantly reduced the content of total flavonoids,quercetin,isoquercitrin and isoquercitrin in C.oleifera leaves by 7.80%,50.00%and 75.90%,respectively.[Conclusions]Our results are an important step which showed strong resistance of C.oleifera and can give a novel insight for researches on the effects in the rhizosphere soil enzyme,soil nutrient elements and metabolites of C.oleifera under the Fusarium sp.too.

Research Progress on Application of Molecular Markers in Breeding of Camellia oleifera

Camellia oleifera is an important woody oil tree species unique to China.It is known as the world s four major woody oil crops along with olive,oil palm and coconut.It is known as the‘king of oil’because of its high oil content.With the increase of people's attention to the yield of Camellia oleifera,its high yield has become the focus.In traditional breeding model,judgment is performed by phenotypic traits,but this method is single and easily affected by the environment,and can no longer meet the demand.In contrast,molecular marker breeding is not affected by the environment,and is stable and efficient and capable of accurately mapping target genes,so it has attracted much attention.In this paper,the research progress on C.oleifera germplasm resources diversity,DNA fingerprinting construction,genetic linkage map construction and QTL mapping was summarized,and the application of SSR molecular marker technique combined with association analysis in C.oleifera breeding in recent years was discussed,in order to provide new ideas for high-yield breeding of C.oleifera.

Plasticizing Effect of Camellia oleifera Seed-Oil-Based Plasticizer on PVC Material Modification

In this study,as the plasticizer,Camellia oleifera seed-oil-based cyclohexyl ester(COSOCE)was prepared by the reaction of cyclohexene oxide and refined C.oleifera seed oil(RCOSO)obtained by acidification hydrolysis after saponification.In addition,the structure of the target product was confirmed by Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy,and Raman spectroscopy.COSOCE was used as plasticizer-modified polyvinyl chloride(PVC)membranes.The structure of the COSOCE-modified PVC membranes were characterized by Raman spectroscopy and scanning electron microscopy(SEM).The properties of the COSOCE-modified PVC membrane were characterized by contact angle measurements,universal testing machine,thermogravimetric analysis(TGA),and differential scanning calorimetry(DSC).The results revealed that(1)The COSOCE-modified PVC membranes exhibit a good microscopic morphology.Combined with energy-dispersive X-ray spectroscopy(EDS)and contact angle measurement results,the COSOCE-modified PVC membranes are confirmed to be a hydrophilic material.(2)The modified PVC membrane with 60%COSOCE exhibited the best mechanical properties.The tensile strength reached 23.56±2.94 MPa.(3)COSOCE-modified PVC material exhibited better thermal stability,with a loss rate of less than 75%at the end of the first decomposition stage.Compared with that of the dioctyl-phthalate(DOP)-modified PVC membrane,the initial decomposition temperature of PVC was increased by 1.17°C–8.17°C,and the residual rate was increased by 0.67%–5.75%.The carbon–carbon double bond in the COSOCE molecular structure can remove the free radicals generated during the degradation of PVC material and slow down the decomposition rate of PVC.In addition,the double bond can be cross-linked partially with the PVC molecular chain containing the conjugated polyene structure,thereby increasing the movement resistance of the PVC molecular chain segment.Hence,COSOCE can replace DOP as a PVC plasticizer.

The Crosslinking Mechanism of Camellia Oleifera Protein Adhesive with Amine Resins by ^(13)C-NMR and ESI-MS

A new protein wood adhesive was studied with Camellia oleifera protein.Formaldehyde and N-(2)-L-Ala-L-Gln(LAG)were used as the model compounds of amino resins and Camellia oleifera protein,aiming to provide scientific foundations for the improvement and applications of Camellia oleifera protein adhesive by the reaction of model compounds.The experimental results demonstrate that,under alkaline conditions,formaldehyde is easier to react with Camellia oleifera protein by quicker reaction and lower curing temperature.Under acid conditions,amino hydroxymethylated structure of aliphatic series from LAG is difficult to form stable reactive intermediates and further polycondensation.Hydroxymethylation of end acylamino and peptide bond amino from LAG is relatively weak.Under alkaline conditions,the free aliphatic amino and acylamino of LAG both can make hydroxymethylation reaction with formaldehyde.In the polycondensation,hydroxymethyl amide is the initial structure and the reactive intermediate is produced by E1cb reaction of hydroxymethyl amide.Methylene bridge bonds and methylene ether bonds are structures of the polycondensation products,which are competing reactions.The former is mainly formed by the reaction between alkaline reactive intermediate and amino of aliphatic series,and the latter is produced by the reaction of reactive intermediate and amino of hydroxymethyl aliphatic series with hydroxymethyl amide.

Evidence of the predominance of passive symplastic phloem loading and sugar transport with leaf ageing in Camellia oleifera

Phloem loading and transport of sugar from leaves to sink tissues such as fruits are crucial for yield formation.Camellia oleifera is an evergreen horticultural crop with high value;however,its low production limits the development of the C.oleifera industry.In this study,using a combination of ultrastructural observation,fluorescence loss in photobleaching(FLIP)and inhibitor treatment,we revealed that C.oleifera leaves mainly adopt a symplastic loading route from mesophyll cells to the surrounding vascular bundle cells in minor veins.HPLC assays showed that sucrose is the main sugar transported and only a small amount of raffinose or stachyose was detected in petioles,supporting a passive symplastic loading route in C.oleifera leaves.Compared to leaves grown this year(LT),the carbohydrate synthesis capacity in leaves grown last year(LL)was decreased while LL retained more soluble sugar,suggesting a decrease in transport capacity with leaf ageing.TEM and tissue staining showed that a reduction in plasmodesmata density leads to a decline in the degree of cellular coupling and is responsible for the weakening transport capacity in older leaves.RNA-seq revealed several differentially expressed genes(DEGs)including CoPDCB1-1,CoSUT1 and CoSWEET12,which are likely involved in the regulation of phloem loading and sugar transport.An expression correlation network is constructed between PD-callose binding protein genes,sugar transporter genes and senescence-associated genes.Collectively,this study provides the evidence of the passive symplastic phloem loading pathway in C.oleifera leaves and constructs the correlation between sugar transport and leaf ageing.

Species and Control Techniques of Main Diseases and Insect Pests of Camellia oleifera in Anhui Province

[Objectives]The paper was to master the species,incidence regularity and control techniques of main diseases and insect pests of Camellia oleifera in Anhui Province.[Methods]The species of main diseases and insect pests of C.oleifera in major C.oleifera afforestation bases and seedling bases in Anhui Province were investigated through field survey and literature search.Afterwards,the symptom characteristics,occurrence regularity and harms of diseases and insect pests were analyzed,and scientific and reasonable control techniques were put forward.[Results]The main diseases of C.oleifera in Anhui Province were soft rot disease,blister blight,anthracnose,sooty blotch,etc.,and the main insect pests were Euproctis pseudoconspersa,Biston marginata,Hypomeces squamosus,Curculio chinensts,Chrenoma atritarsis,etc.The control techniques mainly included ecological regulation,physical prevention and control,chemical prevention and control,and biological prevention and control.[Conclusions]The results will promote the high-quality development of C.oleifera industry in Anhui Province,and contribute to the improvement of China s edible vegetable oil supply and national grain and oil security.

Study on Extraction of Tea Saponin from Camellia oleifera Cake Using Water as Extraction Solvent

This paper studied the effects of liquid-solid ratio, temperature, time and pH value on the extraction rate of tea saponin from the cake of Camellia oleifera seeds by using single factor experiment with the cake of Camellia oleifera seeds as the raw materials, and water as the extraction solvent, and orthogonal test was used to determine the optimal extraction process conditions. The results showed that the extraction ratio of tea saponin could reach up to 95.50% when the liquidsolid ratio was 11：1, extracting temperature of 80 ℃, extraction time of 6 h, and pH value of 9.

A Preliminary Investigation of Low Yield Reason of Camellia oleifera in Dehong Prefecture

[Objective] The paper was to preliminarily investigate the low yield reason of Camellia oleifera caused by fruit and flower dropping in Dehong prefecture. [Method]Pollen viability of C. oleifera was determined using I-KI and TTC （2,3,5-triphenyl tetrazolium chloride） method, the germination rate of pollen was determined by in vitro culture method, the pollen tube growth was observed by the fluorescent microscope through aniline blue dyeing, and the effects of different pollination methods on fruit setting rate of C. oleifera was analyzed. [Results] The pollen viability and the quantity of pollen tube of C. oleifera with good fruiting were significantly higher than the plants with poor fruiting, but their pollen germination rate had no significant differences; cross pollination was conducive to fruiting setting of C. oleifera. [Conclusion] The growth and development condition of C. oleifera pollen had important effect on the yield of C. oleifera in Dehong prefecture.

Optimization of Cultivation Medium for Container Seedling Raising of Camellia oleifera

[Objective] This study aimed to investigate the optimal component of culti- vation medium for container seedling raising of Camellia oleifera. [Method] In the application of container nursery technology of Camellia oleifera grafting in emergent stocks, yellow clay soil, surface soil of pine forest, decomposed edible fungus, peat soil, dung and dirt were mixed by different proportions into five formulae as nursery substrates, with garden soil as the control, in order to investigate the influences of various substrates on the growth of annual Camellia oleifera container seedlings by using randomized block design based on variance analysis and multiple comparisons, and to screen the optimal substrate formula for container nursery of Camellia oleifera. [Result] The influences of different substrates on the transplanting survival rate, seedling height, basal diameter, height-diameter ratio and lateral root length of Camellia oleifera container seedlings were significantly different. Various growth indi- cators of Camellia oleifera container seedlings cultivated in the substrate containing 40% of yellow clay soil ＋ 15% of surface soil of pine forest ＋ 20% of decomposed edible fungus ＋ 20% of peat soil ＋ 5% of dung and dirt exceeded that of the control and other substrate formulae, which could be used as the optimal substrate formula for the container nursery of Camellia oleifera. [Conclusion] This study screened the optimal substrate formula for the container nursery of Camellia oleifera, which provided technical reference for the cultivation of Camellia oleifera container seedlings.

Study on the Redifferentiation Technology after the Dedifferentiation of Blades of Camellia oleifera Abel.

Based on selecting MS minimal medium, through changing the concentrations and additive amounts of two plant growth regulators of NAA and BA, the redifferentiation experiments of plant morphology on callus and embryos cell which got after the dedifferentiation of ＂Xianglin No.1＂ Camellia oleifera Abel. were carried out.The experimental results showed that callus lines with white, yellowish white or oyster colors, obvious uneven surface and loose texture were inoculated on the medium of MS ＋NAA（0.3 mg/L） ＋BA（2.0 mg/L） ＋saccharose（30 g/L） ＋agar（7 g/L）for 30 d,then multiple shoots were differentiated from the white protuberant part, moreover,the growth vigor was good. If inoculating using regeneration buds of C. oleifera, its multiplication coefficient was 6.50.

木本植物适应酸性土壤机理的研究进展——以胡枝子(Lespedeza bicolor)和油茶(Camellia oleifera)为例

综述了近年来木本植物适应酸性土壤的耐铝机理研究进展,重点以酸性土壤先锋植物胡枝子(Lespedeza bicolor)和铝累积植物油茶(Camellia oleifera)为例,总结了木本植物根系有机酸的分泌、铝吸收和运输机制及铝与氮磷胁迫的协同适应等。木本植物有铝累积和铝排斥植物之分;铝排斥植物胡枝子耐铝的重要机制是其根系同时分泌柠檬酸和苹果酸;铝累积植物油茶高效累积铝的原因在于其不仅可以高效吸收土壤和土壤溶液中广泛存在的铝(Al3+和Al-F),而且可以通过木质部运输和特定季节韧皮部运输的配合实现铝的高效分配和传输;铵态氮相对于硝态氮可缓解胡枝子的铝毒害;磷对不同胡枝子耐铝作用的影响明显不同。木本植物适应酸性土壤机理的深入研究将会有助于完善植物的耐铝机理及铝运输理论,并为酸性土壤中矿质养分管理提供理论基础。

Flavonoid triglycosides from the seeds of Camellia oleifera Abel

Two flavonoid triglycosides, kaempferol 3-O-{ β-D-glucopyranosyl-（1→2）-[α-L-rhamnopyranosyl-（1→ 6）]-β-D-glucopyranoside} （1） and kaempferol 3-O-{ β-D-xylopyranosyl-（1 → 2）-[α-L-rhamnopyranosyl-（1→6）]-β-D-glucopyranoside } （2）, were isolated from the seed of Camellia oleifera Abel. The absolute configuration of compound 1 was established on the basis of its X-ray analysis. Their cytotoxic activities and anti-HIV-RT activities were evaluated.

Study on Camellia Oleifera Protein based Wood Adhesive by Epoxy Resin and Its Crosslinking Mechanism

Epoxy resin (EPR) was used to crosslink with Camellia oleifera Abel.protein to prepare wood adhesive,and the bonding performance and curing characteristics of which were mainly investigated,and the synthesis mechanism was also discussed by using model compounds.The experimental results show that EPR can significantly improve the bonding performance of Camellia oleifera Abel.protein-based adhesive,and the maximum of which reaches 0.72 MPa satisfies the strength requirement of Type II plywood in GB/T 17657-2013.After alkali treatment,the protein can more easily crosslink with EPR at low curing temperature,and the adhesive has high degree of crystallinity of curing products,high degree of crosslinking reaction,and high bonding strength.The reaction mechanism of EPR-modified Camellia oleifera Abel.protein adhesive can be divided into resinification phase and curing phase.

Isolation, Identification and Phosphate Solubilizing Capacity of Organophosphorus Solubilizing Bacteria in Rhizosphere Soil of Camellia oleifera

In order to obtain high-efficiency organophosphorus solubilizing bacteria, 21 strains of organophosphate solubilizing bacteria were isolated from the rhizosphere soil of Camellia oleifera plants, and the transparent circle method was used for rescreening. Only 4 strains of bacteria could form transparent circle on organophosphorus medium. The D/d value of 4 strains of bacteria was between 1.62 and 2.71, among which the D/d value of strain Y6 was the highest(2.71). The available phosphorus content of the fermentation supernatant was 8.50~14.79 mg/L, which was 7.88~14.17 mg/L higher than that of CK. The strain Y6 had the highest soluble phosphorus content of 14.79 mg/L in the fermentation supernatant, which was 14.17 mg/L higher than that of CK. According to the colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis of strain Y6, it is preliminarily determined that strain Y6 is Pseudomonas. Strain Y6 is beneficial to improve the supply of phosphorus in rhizosphere soil of Camellia oleifera and promote the growth of Camellia oleifera. It has great potential in the development of bio-organic fertilizer.

Survey on Early-bearing High-yielding Model Plantation of Camellia oleifera

[Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in two demonstration bases, one for investigating the relationship between different cultivated varieties with growth and production, the other for investigating the relationship between different planting densities with growth and production. [Result] For different cultivated varieties, Cenruan 2 showed tree height, ground diameter and crown product significantly higher than other two varieties, besides, the per unit area fruit yield of Cenruan 2 was also significantly higher than other two varieties. From flower bud differentiation,the best variety was Changlin 4, which showed number of differentiated flower buds significantly higher than Changlin 27, but there were no significant differences between Cenruan 2 with Changlin 4 and Changlin 27. For different planting densities, plant height and crown product at planting density of 1.5 m×2 m were significantly higher than those of other two planting densities, but from ground diam- eter, the best planting density was 1.5 m×1.0 m, with a ground diameter significantly higher than other two planting densities. From per unit area fruit yield, the best planting density was 1.5 m×2.0 m, under which early plant growth and fruit production were better. Therefore, the best planting density is 2.0 m ×3.0 m when constructing early-bearing high-yielding model plantations. For variety Cenruan 3, it＇s fruit production is high in the early pedod, and the best planting density is 1.5 m× 2.0 m or 1.0 m×1.5 m when constructing early-bearing high-yielding model plantations, and after 4-6 years when it put into production, the plants could be transplanted.

Construction of Rooting System during Camellia oleifera Tissue Culture

An rooting experiment of tissue culture plantlets was carried out with sterile plantlets obtained from the stem segments of a good clone of Camellia oleifera as materials. The results showed that basic medium and illumination condition are factors crucial to rooting of C. oleifera. With 1/4MS as basic medium, the treat- ment with the addition of 0.5 mg/L NAA and soaking in 2 000 mg/L KIBA, containing 30 mg/L sucrose and subjected to dark culture of 20 d was the optimal treat- ment, achieving a rooting rate of 86. 7%.

Construction of Camellia oleifera Cultivation Standardization System

The Camellia oleifera industry is a rising industry with Chinese characteristics.In recent years,the C.oleifera industry has made great achievements and is also in the development stage of climbing uphill.This paper overviewed the background of standardization,expounded the necessity of the standardization of C.oleifera cultivation,and proposed a new ideas that the construction of C.oleifera cultivation standardization needs to be divided into such six key links as indispensable forest land selection,seedling selection,afforestation technology,fertilization technology,tree cultivation technology and timely harvesting according to the current situation and problems of C.oleifera cultivation standardization.