[Objective] The paper was to preliminarily investigate the low yield reason of Camellia oleifera caused by fruit and flower dropping in Dehong prefecture. [Method]Pollen viability of C. oleifera was determined using I...[Objective] The paper was to preliminarily investigate the low yield reason of Camellia oleifera caused by fruit and flower dropping in Dehong prefecture. [Method]Pollen viability of C. oleifera was determined using I-KI and TTC （2,3,5-triphenyl tetrazolium chloride） method, the germination rate of pollen was determined by in vitro culture method, the pollen tube growth was observed by the fluorescent microscope through aniline blue dyeing, and the effects of different pollination methods on fruit setting rate of C. oleifera was analyzed. [Results] The pollen viability and the quantity of pollen tube of C. oleifera with good fruiting were significantly higher than the plants with poor fruiting, but their pollen germination rate had no significant differences; cross pollination was conducive to fruiting setting of C. oleifera. [Conclusion] The growth and development condition of C. oleifera pollen had important effect on the yield of C. oleifera in Dehong prefecture.展开更多
基金Supported by National Key Technology Support Program"Integration and Demonstration of Camellia oleifera High Yield Cultivation Technology of Southwest High Mountain"(2009BADB1B08)Key New Product Development Projects of Yunnan Province"Breeding of Good Varieties of Camellia oleifera"(2009BB004)~~
文摘[Objective] The paper was to preliminarily investigate the low yield reason of Camellia oleifera caused by fruit and flower dropping in Dehong prefecture. [Method]Pollen viability of C. oleifera was determined using I-KI and TTC （2,3,5-triphenyl tetrazolium chloride） method, the germination rate of pollen was determined by in vitro culture method, the pollen tube growth was observed by the fluorescent microscope through aniline blue dyeing, and the effects of different pollination methods on fruit setting rate of C. oleifera was analyzed. [Results] The pollen viability and the quantity of pollen tube of C. oleifera with good fruiting were significantly higher than the plants with poor fruiting, but their pollen germination rate had no significant differences; cross pollination was conducive to fruiting setting of C. oleifera. [Conclusion] The growth and development condition of C. oleifera pollen had important effect on the yield of C. oleifera in Dehong prefecture.
