Objective To explore the potential molecular targets of Cang Fu Dao Tan Formula(CFDTF)in the treatment of obese polycystic ovary syndrome(PCOS)using network pharmacology and bioinformatic approaches.Methods The potent...Objective To explore the potential molecular targets of Cang Fu Dao Tan Formula(CFDTF)in the treatment of obese polycystic ovary syndrome(PCOS)using network pharmacology and bioinformatic approaches.Methods The potential blood-entry active compounds and targets of CFDTF were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and obese PCOS related gene targets were retrieved from GeneCard databases.A protein-protein interaction(PPI)network of CFDTF component-targets and obese PCOS diseasetargets was constructed using STRING.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of the intersection network were conducted using a Bioconductor database.Results In total,114 active compounds were screened according to oral bioavailability(OB)and drug similarity(DL),and 328 targets related to these constituents were obtained.Further,2559 target genes directly related to obese PCOS were obtained from the GeneCard databases,and 82 genes were obtained from the intersection of the component-target and disease-target PPI network.These genes were mainly involved in response to steroid hormones,nutrient levels and lipopolysaccharide as well as in other biological processes.Their molecular functions were mainly related to nuclear receptor activity,phosphatase binding and cytokine activity,and they were enriched in the cytoplasm,cell membrane and membrane region.Conclusions CFDTF consists of 114 active compounds that are responsible for its pharmacodynamic effects.It mainly regulates the AGE-RAGE signaling pathway in diabetic complications,bladder cancer,and hepatitis B and in other signaling networks.These findings provide certain theoretical and scientific basis for the treatment of obese PCOS with Chinese medicine.展开更多
Based on the data collected at the Cau Da station, the Regional Ocean Modeling System (ROMS) is applied for hydrodynamics (e.g. circulation and temperature-salinity) in Binh Cang - Nha Trang Bay, Vietnam. The results ...Based on the data collected at the Cau Da station, the Regional Ocean Modeling System (ROMS) is applied for hydrodynamics (e.g. circulation and temperature-salinity) in Binh Cang - Nha Trang Bay, Vietnam. The results present the several cases of hydrodynamic regimes in the Bay during two mainly monsoon seasons (northeast and southwest monsoons). The clearly hydrodynamic regime occurs in the bay due to the impacts of rivers and monsoon conditions. These changes could be contributed to the hydrodynamic systems and water exchanges, that support basic scientific information for sustainable development of Binh Cang - Nha Trang bay.展开更多
目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Ph...目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Phenopedia、TTD和PharmGKB数据库获取相关鼻炎的基因。通过STRING数据库与Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络;利用STRING数据库对核心基因进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。通过Cytoscape软件构建“中药成分-靶点-信号通路”网络并筛选核心靶点;采用AutoDock软件对筛选得到的关键通路靶点进行分子对接。结果CRZS有131个核心基因,鼻炎相关靶点756个,交集靶点41个。GO生物富集条目2005条,KEGG通路富集条目151条,HIF-1、Lipid and atherosclerosis、Fluid shear stress and atherosclerosis等通路最具连接可能。分子对接显示,CEZS靶点的有效成分与3个核心基因连接紧密。结论CEZS可通过多成分、多靶点,多信号通路发挥治疗鼻炎的作用,其中最有可能通过HIF-1、lipid and atherosclerosis、Fluid shear stress and atherosclerosis等3条通路进行调控。展开更多
目的实验室应激条件下诱发大学生焦虑情绪,并观察经鼻吸入苍艾挥发油(cang-ai volatile oil,CAVO)对该情绪相关生理指标的影响。方法招募108名符合纳入标准的大学生受试者,随机分为苍艾组、芳樟叶油组和空白组,36例/组。各组受试者均以...目的实验室应激条件下诱发大学生焦虑情绪,并观察经鼻吸入苍艾挥发油(cang-ai volatile oil,CAVO)对该情绪相关生理指标的影响。方法招募108名符合纳入标准的大学生受试者,随机分为苍艾组、芳樟叶油组和空白组,36例/组。各组受试者均以“三位数连续减17”心算应激诱发焦虑情绪,剔除应激不响应者后,芳樟叶油组、苍艾组各30例、空白组31例,3组基线期和应激期焦虑相关指标无统计学差异,均衡可比。除空白组无精油干预外,其余2组分别经鼻吸入芳樟叶油、CAVO,监测试验的基线期、应激期、干预期和恢复期心搏总数、收缩压(systolic blood pressure,SBP)、舒张压(diastolic blood pressure,DBP)和心率变异性低频高频比值(low frequency high frequency ratio,LF/HF),并行组间与组内比较。结果①组内比较:3组应激期LF/HF、心搏总数、SBP和DBP高于基线期(P<0.05);空白组干预期与应激期比较无差异(P>0.05);苍艾组干预期LF/HF、心搏总数、SBP和DBP低于应激期(P<0.05),且与基线期、恢复期无明显差异(P>0.05);芳樟叶油组干预期LF/HF、SBP和DBP低于应激期(P<0.05),高于基线期和恢复期(P<0.05);②组间比较:苍艾组干预期LF/HF、SBP低于其它2组,DBP低于空白组(P<0.05)。结论实验室应激条件下,经鼻吸入CAVO后大学生焦虑情绪相关生理指标显著下降,可缓解应激诱发的焦虑情绪。展开更多
基金We thank for the funding support from the the Scientific Research Projects of Shanghai Science and Technology Commission(No.19401930200).
文摘Objective To explore the potential molecular targets of Cang Fu Dao Tan Formula(CFDTF)in the treatment of obese polycystic ovary syndrome(PCOS)using network pharmacology and bioinformatic approaches.Methods The potential blood-entry active compounds and targets of CFDTF were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and obese PCOS related gene targets were retrieved from GeneCard databases.A protein-protein interaction(PPI)network of CFDTF component-targets and obese PCOS diseasetargets was constructed using STRING.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis of the intersection network were conducted using a Bioconductor database.Results In total,114 active compounds were screened according to oral bioavailability(OB)and drug similarity(DL),and 328 targets related to these constituents were obtained.Further,2559 target genes directly related to obese PCOS were obtained from the GeneCard databases,and 82 genes were obtained from the intersection of the component-target and disease-target PPI network.These genes were mainly involved in response to steroid hormones,nutrient levels and lipopolysaccharide as well as in other biological processes.Their molecular functions were mainly related to nuclear receptor activity,phosphatase binding and cytokine activity,and they were enriched in the cytoplasm,cell membrane and membrane region.Conclusions CFDTF consists of 114 active compounds that are responsible for its pharmacodynamic effects.It mainly regulates the AGE-RAGE signaling pathway in diabetic complications,bladder cancer,and hepatitis B and in other signaling networks.These findings provide certain theoretical and scientific basis for the treatment of obese PCOS with Chinese medicine.
文摘Based on the data collected at the Cau Da station, the Regional Ocean Modeling System (ROMS) is applied for hydrodynamics (e.g. circulation and temperature-salinity) in Binh Cang - Nha Trang Bay, Vietnam. The results present the several cases of hydrodynamic regimes in the Bay during two mainly monsoon seasons (northeast and southwest monsoons). The clearly hydrodynamic regime occurs in the bay due to the impacts of rivers and monsoon conditions. These changes could be contributed to the hydrodynamic systems and water exchanges, that support basic scientific information for sustainable development of Binh Cang - Nha Trang bay.
文摘目的基于网络药理学和分子对接技术探究苍耳子散(CRZS)治疗异质性鼻炎的作用机制以及“多成分-多靶点-多通路”的整体药理作用特征。方法利用中药系统药理学数据库与分析平台(TCMSP)检索CRZS的活性成分及靶点;从GeneCards、DisGeNET、Phenopedia、TTD和PharmGKB数据库获取相关鼻炎的基因。通过STRING数据库与Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络;利用STRING数据库对核心基因进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。通过Cytoscape软件构建“中药成分-靶点-信号通路”网络并筛选核心靶点;采用AutoDock软件对筛选得到的关键通路靶点进行分子对接。结果CRZS有131个核心基因,鼻炎相关靶点756个,交集靶点41个。GO生物富集条目2005条,KEGG通路富集条目151条,HIF-1、Lipid and atherosclerosis、Fluid shear stress and atherosclerosis等通路最具连接可能。分子对接显示,CEZS靶点的有效成分与3个核心基因连接紧密。结论CEZS可通过多成分、多靶点,多信号通路发挥治疗鼻炎的作用,其中最有可能通过HIF-1、lipid and atherosclerosis、Fluid shear stress and atherosclerosis等3条通路进行调控。
文摘目的实验室应激条件下诱发大学生焦虑情绪,并观察经鼻吸入苍艾挥发油(cang-ai volatile oil,CAVO)对该情绪相关生理指标的影响。方法招募108名符合纳入标准的大学生受试者,随机分为苍艾组、芳樟叶油组和空白组,36例/组。各组受试者均以“三位数连续减17”心算应激诱发焦虑情绪,剔除应激不响应者后,芳樟叶油组、苍艾组各30例、空白组31例,3组基线期和应激期焦虑相关指标无统计学差异,均衡可比。除空白组无精油干预外,其余2组分别经鼻吸入芳樟叶油、CAVO,监测试验的基线期、应激期、干预期和恢复期心搏总数、收缩压(systolic blood pressure,SBP)、舒张压(diastolic blood pressure,DBP)和心率变异性低频高频比值(low frequency high frequency ratio,LF/HF),并行组间与组内比较。结果①组内比较:3组应激期LF/HF、心搏总数、SBP和DBP高于基线期(P<0.05);空白组干预期与应激期比较无差异(P>0.05);苍艾组干预期LF/HF、心搏总数、SBP和DBP低于应激期(P<0.05),且与基线期、恢复期无明显差异(P>0.05);芳樟叶油组干预期LF/HF、SBP和DBP低于应激期(P<0.05),高于基线期和恢复期(P<0.05);②组间比较:苍艾组干预期LF/HF、SBP低于其它2组,DBP低于空白组(P<0.05)。结论实验室应激条件下,经鼻吸入CAVO后大学生焦虑情绪相关生理指标显著下降,可缓解应激诱发的焦虑情绪。
