This paper expresses the efficient outputs of decisionmaking unit（DMU） as the sum of ＂average outputs＂ forecasted by a GM（1,N） model and ＂increased outputs＂ which reflect the difficulty to realize efficient ou...This paper expresses the efficient outputs of decisionmaking unit（DMU） as the sum of ＂average outputs＂ forecasted by a GM（1,N） model and ＂increased outputs＂ which reflect the difficulty to realize efficient outputs.The increased outputs are solved by linear programming using data envelopment analysis efficiency theories,wherein a new sample is introduced whose inputs are equal to the budget in the issue No.n ＋ 1 and outputs are forecasted by the GM（1,N） model.The shortcoming in the existing methods that the forecasted efficient outputs may be less than the possible actual outputs according to developing trends of input-output rate in the periods of pre-n is overcome.The new prediction method provides decision-makers with more decisionmaking information,and the initial conditions are easy to be given.展开更多
为研究免疫抑制信号通路程序性细胞死亡因子-1(programed cell death 1, PD-1)及其配体-1(ligands of programmed death-1,PD-L1)在犬肿瘤等免疫抑制类疾病中发挥的作用,进一步寻求治疗肿瘤的方法和手段,试验根据在GenBank中查询的犬PD-...为研究免疫抑制信号通路程序性细胞死亡因子-1(programed cell death 1, PD-1)及其配体-1(ligands of programmed death-1,PD-L1)在犬肿瘤等免疫抑制类疾病中发挥的作用,进一步寻求治疗肿瘤的方法和手段,试验根据在GenBank中查询的犬PD-1及PD-L1基因序列信息,结合生物信息学技术,以及在其他种源中二者蛋白质结构研究的相关结果,克隆编码胞外区主要结构域的基因片段,构建相应原核表达载体并诱导表达,并应用Western-blot对目标蛋白质产物进行鉴定。结果表明:成功构建了表达犬PD-1(25~168 aa)及PD-L1(19~238 aa)胞外区主要结构域的原核重组表达载体pET-30a-PD-1和pET-30a-PD-L1,诱导表达的目标蛋白质产物分子质量分别约为27 ku和33 ku,二者均以包涵体形式表达,纯化后蛋白质产物纯度可达到80%,且均能够被商品化抗体特异性识别,具有良好的免疫原性。展开更多
基金supported by the Research Start Funds for Introducing High-level Talents of North China University of Water Resources and Electric Power
文摘This paper expresses the efficient outputs of decisionmaking unit（DMU） as the sum of ＂average outputs＂ forecasted by a GM（1,N） model and ＂increased outputs＂ which reflect the difficulty to realize efficient outputs.The increased outputs are solved by linear programming using data envelopment analysis efficiency theories,wherein a new sample is introduced whose inputs are equal to the budget in the issue No.n ＋ 1 and outputs are forecasted by the GM（1,N） model.The shortcoming in the existing methods that the forecasted efficient outputs may be less than the possible actual outputs according to developing trends of input-output rate in the periods of pre-n is overcome.The new prediction method provides decision-makers with more decisionmaking information,and the initial conditions are easy to be given.
