[Objective] This study aimed to construct the recombinant expression plasmids containing H and F protein genes of Canine distemper virus isolated from a mink and to express these two genes in prekaryotic cells as well...[Objective] This study aimed to construct the recombinant expression plasmids containing H and F protein genes of Canine distemper virus isolated from a mink and to express these two genes in prekaryotic cells as well as to study the reactogenieity of the expressed products. [ Method ] RT-PCR amplification was used to obtain H and F protein genes; TA cloning and subclonlng techniques were used to construct the cloning plasmids(pMD-18T-H and pMD-18T-F) and recombinant expression plasmids(pET28a-H and pET28a-F) ; SDS-PAGE and Western-blotting were adopted to verify whether the target proteins were successfully expressed. [ Result] The recombinant expression plasmids pET28a-H and pET28a-F containing H and F protein genes of Canine distemper virus isolated from a mink were successfully constructed, and both the expressed H and F proteins with respectively relative molecular mass of 31 400 and 38 200 produced positive reac- tion with the CDV standard positive serum. [ Conclusion] The H and F proteins expressed in prokaryotic cells were the same with the natural ones in terms of reac- togenicity, which can be utilized for diagnosis of a CDV's infection or for an epidemiological investigation. Meanwhile, they also provide a basis for developing ge- netically engineered subunit vaccines.展开更多
Canine distemper virus(CDV)has recently been identified in populations of wild tigers in Russia and India.Tiger populations are generally too small to maintain CDV for long periods,but are at risk of infections arisin...Canine distemper virus(CDV)has recently been identified in populations of wild tigers in Russia and India.Tiger populations are generally too small to maintain CDV for long periods,but are at risk of infections arising from more abundant susceptible hosts that constitute a reservoir of infection.Because CDV is an additive mortality factor,it could represent a significant threat to small,isolated tiger populations.In Russia,CDV was associated with the deaths of tigers in 2004 and 2010,and was coincident with a localized decline of tigers in Sikhote-Alin Biosphere Zapovednik(from 25 tigers in 2008 to 9 in 2012).Habitat continuity with surrounding areas likely played an important role in promoting an ongoing recovery.We recommend steps be taken to assess the presence and the impact of CDV in all tiger range states,but should not detract focus away from the primary threats to tigers,which include habitat loss and fragmentation,poaching and retaliatory killing.Research priorities include:(i)recognition and diagnosis of clinical cases of CDV in tigers when they occur;and(ii)collection of baseline data on the health of wild tigers.CDV infection of individual tigers need not imply a conservation threat,and modeling should complement disease surveillance and targeted research to assess the potential impact to tiger populations across the range of ecosystems,population densities and climate extremes occupied by tigers.Describing the role of domestic and wild carnivores as contributors to a local CDV reservoir is an important precursor to considering control measures.展开更多
The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canin...The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canine distemper virus(CDV).We use a population viability analysis metamodel,which consists of a traditional individual-based demographic model linked to an epidemiological model,to assess options for controlling the impact of negative factors through domestic dog management in protected areas,increasing connectivity to the neighboring large population(including more than 400 individuals),and habitat expansion.Without intervention,under inbreeding depression of 3.14,6.29,and 12.26 lethal equivalents,our metamodel predicted the extinction within 100 years is 64.4%,90.6%,and 99.8%,respectively.In addition,the simulation results showed that dog management or habitat expansion independently will not ensure tiger population viability for the next 100 years,and connectivity to the neighboring population would only keep the population size from rapidly declining.However,when the above three conservation scenarios are combined,even at the highest level of 12.26 lethal equivalents inbreeding depression,population size will not decline and the probability of extinction will be<5.8%.Our findings highlight that protecting the Amur tiger necessitates a multifaceted synergistic effort.Our key management recommendations for this population underline the importance of reducing CDV threats and expanding tiger occupancy to its former range in China,but re-establishing habitat connectivity to the neighboring population is an important long-term objective.展开更多
Poaching as well as loss of habitat and prey are identified as causes of tiger population declines.Although some studies have examined habitat requirements and prey availability,few studies have quantified cause-speci...Poaching as well as loss of habitat and prey are identified as causes of tiger population declines.Although some studies have examined habitat requirements and prey availability,few studies have quantified cause-specific mortality of tigers.We used cumulative incidence functions(CIFs)to quantify cause-specific mortality rates of tigers,expanding and refining earlier studies to assess the potential impact of a newly emerging disease.To quantify changes in tiger mortality over time,we re-examined data first collected by Goodrich et al.(2008;study period 1:1992–2004)as well as new telemetry data collected since January 2005(study period 2:2005–2012)using a total of 57 tigers(27 males and 30 females)monitored for an average of 747 days(range 26–4718 days).Across the entire study period(1992 to 2012)we found an estimated average annual survival rate of 0.75 for all tigers combined.Poaching was the primary cause of mortality during both study periods,followed by suspected poaching,distemper and natural/unknown causes.Since 2005,poaching mortality has remained relatively constant and,if combined with suspected poaching,may account for a loss of 17–19%of the population each year.Canine distemper virus(CDV)may be an additive form of mortality to the population,currently accounting for an additional 5%.Despite this relatively new source of mortality,poaching remains the main threat to Amur tiger survival and,therefore,population growth.展开更多
基金Supported by the Natural Science Foundation of Jilin Province(201115194)Education Department of Jilin Province(2009.No.66)
文摘[Objective] This study aimed to construct the recombinant expression plasmids containing H and F protein genes of Canine distemper virus isolated from a mink and to express these two genes in prekaryotic cells as well as to study the reactogenieity of the expressed products. [ Method ] RT-PCR amplification was used to obtain H and F protein genes; TA cloning and subclonlng techniques were used to construct the cloning plasmids(pMD-18T-H and pMD-18T-F) and recombinant expression plasmids(pET28a-H and pET28a-F) ; SDS-PAGE and Western-blotting were adopted to verify whether the target proteins were successfully expressed. [ Result] The recombinant expression plasmids pET28a-H and pET28a-F containing H and F protein genes of Canine distemper virus isolated from a mink were successfully constructed, and both the expressed H and F proteins with respectively relative molecular mass of 31 400 and 38 200 produced positive reac- tion with the CDV standard positive serum. [ Conclusion] The H and F proteins expressed in prokaryotic cells were the same with the natural ones in terms of reac- togenicity, which can be utilized for diagnosis of a CDV's infection or for an epidemiological investigation. Meanwhile, they also provide a basis for developing ge- netically engineered subunit vaccines.
基金We would like to thank the Morris Animal Foundation,Zoo Boise,and the Biotechnology and Biological Sciences Research Council for their generous support of the project.In addition,none of this work would have been possible without the continued partnership of the Sikhote-Alin Biosphere Zapovednik(Director D.Yu.Gorskhov),Lazovskii Zapovednik(Director A.A.Laptev)and the Russian Ministry of Natural Resources.Thanks also to V.Keahey(In-Sync Exotics)for insights into the epidemiology of CDV.
文摘Canine distemper virus(CDV)has recently been identified in populations of wild tigers in Russia and India.Tiger populations are generally too small to maintain CDV for long periods,but are at risk of infections arising from more abundant susceptible hosts that constitute a reservoir of infection.Because CDV is an additive mortality factor,it could represent a significant threat to small,isolated tiger populations.In Russia,CDV was associated with the deaths of tigers in 2004 and 2010,and was coincident with a localized decline of tigers in Sikhote-Alin Biosphere Zapovednik(from 25 tigers in 2008 to 9 in 2012).Habitat continuity with surrounding areas likely played an important role in promoting an ongoing recovery.We recommend steps be taken to assess the presence and the impact of CDV in all tiger range states,but should not detract focus away from the primary threats to tigers,which include habitat loss and fragmentation,poaching and retaliatory killing.Research priorities include:(i)recognition and diagnosis of clinical cases of CDV in tigers when they occur;and(ii)collection of baseline data on the health of wild tigers.CDV infection of individual tigers need not imply a conservation threat,and modeling should complement disease surveillance and targeted research to assess the potential impact to tiger populations across the range of ecosystems,population densities and climate extremes occupied by tigers.Describing the role of domestic and wild carnivores as contributors to a local CDV reservoir is an important precursor to considering control measures.
基金This work was supported by the National Natural Science Foundation of China(31971539)the National Science and Technology Basic Resources Survey Program of China(2019FY101700)a scholarship from the China Scholarship Council(202106040062).
文摘The continuation of the isolated Amur tiger(Panthera tigris altaica)population living along the China-Russia border is facing serious challenges due to factors such as its small size(including 38 individuals)and canine distemper virus(CDV).We use a population viability analysis metamodel,which consists of a traditional individual-based demographic model linked to an epidemiological model,to assess options for controlling the impact of negative factors through domestic dog management in protected areas,increasing connectivity to the neighboring large population(including more than 400 individuals),and habitat expansion.Without intervention,under inbreeding depression of 3.14,6.29,and 12.26 lethal equivalents,our metamodel predicted the extinction within 100 years is 64.4%,90.6%,and 99.8%,respectively.In addition,the simulation results showed that dog management or habitat expansion independently will not ensure tiger population viability for the next 100 years,and connectivity to the neighboring population would only keep the population size from rapidly declining.However,when the above three conservation scenarios are combined,even at the highest level of 12.26 lethal equivalents inbreeding depression,population size will not decline and the probability of extinction will be<5.8%.Our findings highlight that protecting the Amur tiger necessitates a multifaceted synergistic effort.Our key management recommendations for this population underline the importance of reducing CDV threats and expanding tiger occupancy to its former range in China,but re-establishing habitat connectivity to the neighboring population is an important long-term objective.
文摘Poaching as well as loss of habitat and prey are identified as causes of tiger population declines.Although some studies have examined habitat requirements and prey availability,few studies have quantified cause-specific mortality of tigers.We used cumulative incidence functions(CIFs)to quantify cause-specific mortality rates of tigers,expanding and refining earlier studies to assess the potential impact of a newly emerging disease.To quantify changes in tiger mortality over time,we re-examined data first collected by Goodrich et al.(2008;study period 1:1992–2004)as well as new telemetry data collected since January 2005(study period 2:2005–2012)using a total of 57 tigers(27 males and 30 females)monitored for an average of 747 days(range 26–4718 days).Across the entire study period(1992 to 2012)we found an estimated average annual survival rate of 0.75 for all tigers combined.Poaching was the primary cause of mortality during both study periods,followed by suspected poaching,distemper and natural/unknown causes.Since 2005,poaching mortality has remained relatively constant and,if combined with suspected poaching,may account for a loss of 17–19%of the population each year.Canine distemper virus(CDV)may be an additive form of mortality to the population,currently accounting for an additional 5%.Despite this relatively new source of mortality,poaching remains the main threat to Amur tiger survival and,therefore,population growth.