Preliminary Studies on the Tissue Culture of Cannabis sativa L.(Industrial hemp)

The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on.Hemp seed disinfection used 75% alcohol for 2 min and sterilized in 1‰ Hg Cl2 for 5min. The best combinations of plant growth regulators were 1.0 mg/L 6-BA and 0.5mg/L NAA for the induction of callus, and the best combinations of hormones were1.0 mg/L KT and 0.5 mg/L NAA for differentiation rate of adventitious bud.

A Comparative Study on Hemp(Cannabis sativa)Essential Oil Extraction Using Traditional and Advanced Techniques

A comparative study of Cannabis sativa(Hemp)essential constituents obtained by using Supercritical Fluid Extraction(SCFE),Steam Distillation(SD)and Hydrodistillation(HD)is presented here.The optimized extraction temperatures were 130,110and 50℃for hydrodistillation,steam distillation and supercritical fluid extraction respectively.The essential oil of C.sativa was analyzed by using Gas chromatography mass spectrometry(GC-MS).A total of 33,30and 31components have been identified in HD,SD and SCFE respectively.Yield of essential oil using SCFE(0.039%)was more than HD(0.025%)and SD(0.035%)extraction respectively.The main component of sesquiterpenes obtained by hydrodistillation at 130℃with their percentages included caryophyllene(40.58%),trans-α-bergamotene(5.41%),humulene(10.97%),cis-β-farnesene(8.53%)and monoterpenes includedα-pinene(2.13%),d-limonene(6.46%),p-cymol(0.65%)and cineole(2.58%)respectively.The main component of sesquiterpenes obtained by SD steam distillation at110℃including caryophyllene(38.60%)trans-α-bergamotene(4.22%),humulene(10.26%),cis-β-farnesene(6.67%)and monoterpenes includedα-pinene(3.21%),d-limonene(7.07%),p-cymol(2.59%)and cineole(3.88%)whereas the more percentages of major components were obtained by SCFE at 50℃included caryophyllene(44.31%),trans-α-bergamotene(6.79%),humulene(11.97%)cis-β-farnesene(9.71%)and monoterpenes includedα-pinene(0.45%),d-limonene(2.13%)p-cymol(0.19%)and cineole(1.38%)respectively.We found yield/efficiency,chemical composition,quality of the essential oils by supercritical fluid extraction superior in terms of modern,green,saving energy and a rapid approach as compared to traditional techniques.

镉胁迫对线麻(CannabisSativaL.)富集及光合特性的影响

选择广泛栽培于西北地区的线麻(Cannabis Sativa L.)进行盆栽实验,研究了Cd胁迫对线麻富集和光合特性的影响.结果显示,线麻根、茎、叶中Cd最大富集量分别达到503.40、350.63、77.90 mg·kg^(-1).随着土壤Cd浓度的增加,线麻根、茎、叶的富集量逐渐增加,且表现为:根部>茎部>叶部.当Cd≥150 mg·kg^(-1)时,Cd从根部向地上部分的转运开始加强,且地上部分组织中主要集中在茎部.线麻株高、比叶重和生物量随Cd浓度的增加先升高后下降.当Cd浓度≥150 mg·kg^(-1)时,株高、比叶重和地下部分生物量明显低于对照(P<0.05),而地上部分生物量不存在显著性差异(P>0.05).当Cd浓度为50 mg·kg^(-1)时线麻叶绿素含量、类胡萝卜素含量、净光合速率、蒸腾速率、气孔导度、最大净光合速率和光饱和点达到最大值,光补偿点随Cd浓度的增加而持续增加.结果表明,线麻具有较高的Cd富集能力,可以作为潜在的Cd污染土壤修复栽培作物,但野外修复中如何提高线麻的生物量有待于进一步研究.

Study on the Anti-inflammatory and Repairing Effects of Cannabis Sativa Leaf Extract by Epikutis Model Substitution

To explore the anti-inflammatory effect of Cannabis sativa leaf extracts and their repair to damaged skin,a 3D epidermal model(Epikutis®)was used.During the experiment,after epithelial cell surface was pretreated with SLS for 24 h,the anti-inflammatory effect and repair effect of Cannabis sativa leaf extract were observed by testing tissue viability and the representative pro-inflammatory factors such as IL-1α,TNF-αand IL-8.The tissue viability was detected by the MTT test.The results showed that the Cannabis sativa leaf extract effectively inhibited the secretion of inflammatory factors IL-1α,TNF-αand IL-8,and the tissue viability of the skin model was significantly improved after using the sample.The Cannabis sativa leaf extract has a repairing effect on skin barrier damage by inhibiting skin inflammatory reaction.

<i>Cannabis sativa</i>L. Response to Narrow Bandwidth UV and the Combination of Blue and Red Light during the Final Stages of Flowering on Leaf Level Gas-Exchange Parameters, Secondary Metabolite Production, and Yield

Many modifications to growing environments and specific stress induction techniques exist which are supported by preliminary research to increase yield and/or concentration of secondary metabolites in Cannabis sativa L. One such technique is the modification of the spectral composition of light late in flowering-stage development. In this study, we evaluated the hypothesis that the addition of narrow bandwidth UV-A light and the combination of narrow bandwidth blue and red light, during the final two weeks of the flowering cycle affect the concentration of cannabinoids and terpenes, with no difference in harvest dry weight. Three treatments were used in this experimental design, 1) control-full spectrum illumination, 2) UV-A (~390 nm) at ~66 PPFD in addition to control lighting, and 3) blue (~450 nm) and red (~660 nm) at ~158 PPFD in addition to control lighting. Light treatments were initiated during the final two weeks of flowering to assess secondary metabolite concentration (cannabinoids and terpenes) and yield of three cannabis cultivars (Type I (Larry OG), Type II (Pootie Tang), and Type III (Super White)). With two of the three cultivars used in this study responding with enhanced production of THC our results support the potential beneficial effects of attributed light-mediated treatments on cannabinoid production. On the other hand, our results show contrasting trends for terpene production and yield of dry weight flower.

栽培密度和收获期对工业大麻“云麻7号”主要农艺性状及花叶产量的影响

为了探明花叶用工业大麻在云南地区种植的最佳栽培密度和收获期,以云南主栽的工业大麻品种“云麻7号”为材料,分别于2019、2020年在云南省曲靖市沾益区进行大田随机区组试验。两年均设置4个栽培密度(0.5、1、2、3万株/hm^(2))和4个收获期(140、150、160、170 d),在收获期测定株高、茎粗、花叶产量、茎秆产量以及CBD含量等指标。于170 d收获时,两年的株高均在密度2万株/hm^(2)下达到最大值,分别为346.8 cm和267.1 cm,两年的茎粗均在密度0.5万株/hm^(2)下达到最大值,分别为44.3 mm和49.3 mm。2019年在140 d收获和3万株/hm^(2)密度条件下,花叶产量达到最大值3375 kg/hm^(2),2020年在170 d收获和2万株/hm^(2)密度条件下,花叶产量达到最大值5426 kg/hm^(2)。2019年在150 d收获和3万株/hm^(2)密度条件下,茎秆产量和地上部总产量均达到最大值,分别为7560 kg/hm^(2)和11014 kg/hm^(2),2020年在170 d收获2万株/hm^(2)密度条件下,茎秆产量达到最大值5632 kg/hm^(2),在170 d收获和3万株/hm^(2)密度下,地上部总产量达到最大值11001 kg/hm^(2)。大麻CBD含量两年均在160 d收获和1万株/hm^(2)密度条件下达到最大值,分别为1.2%和2.4%。栽培密度与收获期能影响工业大麻的花叶产量和CBD含量,栽培密度为3万株/hm^(2)和170 d时收获是“云麻7号”获得高产的重要栽培措施,同时在160 d收获和1万株/hm^(2)密度下栽培是获得高CBD含量的关键因素。

工业大麻中大麻二酚酸脱羧转化体系研究

为揭示工业大麻中大麻二酚酸(cannabidiolic acid,CBDA)脱羧转化机理,本研究以工业大麻(Cannabis sativa L.)为原料,采用基团贡献法和Watson公式获得工业大麻中CBDA脱羧转化生成大麻二酚(cannabidiol,CBD)过程中各组分基本热力学参数。在此基础上,根据经典热力学公式确定40~140℃温度范围内工业大麻中CBDA转化生成CBD反应吉布斯自由能,并得出反应平衡常数及平衡转化率。通过热脱羧转化实验,分析温度及工业大麻花叶含水量对CBDA脱羧转化的影响,结合CBD转化生成率最终确定大麻CBDA热脱羧转化机理函数模型及转化活化能。研究结果表明:在40~140℃温度范围内,CBDA热脱羧转化可自发进行,且随着温度升高自发进行趋势越大。在热脱羧转化过程中,CBD转化率增长与温度和含水量呈正相关。通过动力学函数模型拟合,工业大麻花叶中CBDA脱羧转化最概然模型符合F 1模型,转化活化能为83.77 kJ/mol。综上所述,在40~140℃温度范围内工业大麻中CBDA可自主脱羧转化生成CBD,研究结果可为工业大麻产业化加工获得高含量CBD条件选择提供理论支持和实验支撑。

工业大麻内生真菌HDM07挥发性成分分析

旨在开发利用工业大麻内生真菌HDM07挥发性成分,探索工业大麻内生真菌HDM07次生代谢产物生物合成途径。以工业大麻内生真菌HDM07为研究对象,采用GC-MS法和检索质谱数据库分析鉴定内生真菌HDM07发酵液和菌丝体中的挥发性成分,采用形态学观察法和18S rDNA序列分析法鉴定菌种。结果表明,经GC-MS法和质谱数据库检索分析,从内生真菌HDM07发酵液中鉴定出11种挥发性成分,在内生真菌HDM07菌丝体中鉴定出14种挥发性成分,工业大麻叶中鉴定了12种成分,空白对照品溶液(PDB培养基)中鉴定了7种成分;经鉴定内生真菌HDM07为链格孢菌(Alternaria alternate)。上述结果表明,HDM07发酵液挥发性成分与菌丝体中挥发性成分两者的合成途径既有联系又有区别。内生真菌HDM07的发酵液及菌丝体中均含有与工业大麻相同或相似的挥发性成分,也含有其特有的挥发性成分,说明该菌株与宿主植物工业大麻在合成挥发性成分方面具有一定的相关性。

不同品种紫花苜蓿(Medicago sativa)的化感效应研究

用砂培法研究紫花苜蓿(Medicago sativa)的六个品种苗期地上部分水浸提液对鸭茅(Dactylis glomerataL.)的化感效应。结果表明,紫花苜蓿六个品种茎叶水浸提液对鸭茅的化感效应在供试品种间差异显著。游客、苜蓿对鸭茅的种子发芽及幼苗生长有抑制作用,但游客却对鸭茅的苗干重有促进作用;三得利和赛迪对鸭茅的种子发芽、根长及根干重有抑制作用,却对苗生长的影响无显著差异。此外,供试紫花苜蓿浸提液对鸭茅的化感作用,随着浓度的升高,抑制作用随之增强。

内生真菌RP4发酵对工业大麻三种主要大麻素含量的影响

本研究旨在通过甘草内生真菌RP4菌株对工业大麻进行发酵,以高效生产大麻二酚(CBD)原料。选取其3种主要大麻素:CBD、CBDA和THC为研究对象,并使用高效液相色谱法分析了发酵过程中这3种大麻素的含量动态变化。研究发现,在发酵过程中,CBD和THC的含量均呈现先增加后降低的趋势,当发酵时间为3 d时,CBD和THC的含量分别达到最大值5.8643±0.0726和0.4878±0.0002 mg/g,且THC的增加始终保持在国家管控的范围内(THC<0.3%),而CBDA含量持续下降,尤其在发酵第1天内降低幅度最大。因工业大麻产地差异以及选取的实验部位差异,经发酵后其大麻素成分含量增加也有所不同。上述结果说明,采用微生物发酵技术,可以特定提高工业大麻中CBD的含量,从而提高工业大麻资源的利用率,并为CBD以及其他次生代谢产物的提取分离奠定了基础。

外源硅对工业大麻生长及生理特性的影响

为了明确外源硅对工业大麻生长的影响,研究以工业大麻品种云麻7号为材料,采用盆栽的方法,研究5个不同浓度(4、8、12、16、20 g/L)硅酸钠(Na_(2)SiO_(3)9H_(2)O)叶面喷施处理对工业大麻农艺性状、生理特性的影响以及硅在大麻植株中的含量分布。结果表明:施用硅酸钠可不同程度提高工业大麻的株高、茎粗和生物量;同时,硅酸钠能够增强大麻叶片的光合作用,提高其超氧化物歧化酶(SOD)、过氧化物酶(POD)的活性以及渗透调节物质的积累。其中,以12 g/L硅酸钠处理促进工业大麻生长的效果最佳。最后,通过对大麻植株各部位硅含量分析发现,叶面喷施硅酸钠处理后,硅主要富集于大麻叶片,且随着硅酸钠处理浓度的增加,硅在大麻各部位的含量也呈增加趋势。以上结果表明,外源硅能够影响工业大麻生长与生理活动。研究结果将为工业大麻合理地施用硅肥提供科学依据。

Overcoming the Bell‐Shaped Dose‐Response of Cannabidiol by Using Cannabis Extract Enriched in Cannabidiol

Cannabidiol (CBD), a major constituent of Cannabis, has been shown to be a powerful anti-inflammatory and anti-anxiety drug, without exerting a psychotropic effect. However, when given either intraperitoneally or orally as a purified product, a bell-shaped dose-response was observed, which limits its clinical use. In the present study, we have studied in mice the anti-inflammatory and anti-nociceptive activities of standardized plant extracts derived from the Cannabis sativa L., clone 202, which is highly enriched in CBD and hardly contains any psychoactive ingredients. In stark contrast to purified CBD, the clone 202 extract, when given either intraperitoneally or orally, provided a clear correlation between the anti-inflammatory and anti-nociceptive responses and the dose, with increasing responses upon increasing doses, which makes this plant medicine ideal for clinical uses. The clone 202 extract reduced zymosan-induced paw swelling and pain in mice, and prevented TNFα production in vivo. It is likely that other components in the extract synergize with CBD to achieve the desired anti-inflammatory action that may contribute to overcoming the bell-shaped dose-response of purified CBD. We therefore propose that Cannabis clone 202 (Avidekel) extract is superior over CBD for the treatment of inflammatory conditions.

Allelopathic Potential of Rice （Oryza sativa L.） Cultivars on Barnyard Grass （Echinochloa crus-galh~

Laboratory and greenhouse studies were conducted to assess the allelopathic potential of 12 rice cultivars on barnyard grass. Polyethylene glycol （PEG） was used to determine the influence of osmotic potential on the bioassay materials. Effect of different concentrations of stem, root, and leaf extracts of rice cultivars on seed germination, radicle and primary shoot length of barnyard grass seedlings, and rate of respiration of root pieces were investigated in the laboratory experiments. Shoot height and dry weight of weed stands were studied in the greenhouse, Also total peroxidase activity, chlorophyll pigment and mitotic index were determined. Results indicated that, among rice cultivars, Mehr, Tarom-mahali, G3, Nemat, and Shahpasand caused the most inhibition effects on investigated factors. Amol-3 showed the least negative effects on growth of seedlings and stands of barnyard grass. In laboratory, the Mehr cultivar demonstrated the maximum inhibitory effects by reducing barnyard grass seed germination percentage （88%）, radicle length （100%）, primary shoot length （83%）, and root respiration （85%） Cell division, expressed as mitotic index, was significantly reduced in the presence of rice aqueous extracts. Mehr cultivar had higher inhibitory effect on mitosis compared to Amol-3. In greenhouse, the same cultivar showed the maximum inhibitory effect by reducing barnyard grass height （45%） and dry weight （64%）. With increase in extract concentration, the inhibitory effect increased. Leaf extract from rice plants was more effective compared with the root and stem extracts. Comparison of rice cultivars and PEG indicated that any reduction in germination and growth of barnyard grass using rice extract concentrations of 5% and 10% must have been the result of allelochemicals in the extracts. Lower extract concentrations showed a more pronounced inhibitory effect in the laboratory as compared to the greenhouse studies. These results suggest that rice leaf extracts may be a source of natural herbicide.

Folic Acid Reverses the Effects of Cannabis on the Brain of New Born Wistar Rats

There are conflicting reports on the effect of Cannabis on brain cells, some reports support damage to brain cells while others do not. This work was carried out at the neuroscience unit of the Department of Veterinary Anatomy, University of Ibadan Oyo State, Nigeria. It investigated the neurodegenerative effect of extract of Cannabis sativa plant on brain regions in newborn Wistar rats exposed to Cannabis in utero and also combining the antioxidant and cell proliferative properties of folic acid to reverse these changes particularly in the Purkinje layer of the cerebellum in the group that received folic acid and cannabis. Four groups of pregnant Wistar rats were treated from Day 5 to 20 of pregnancy as follows: group A received 2ml of normal saline solution per os, group B received 2.0 mg /kg body weight of ethanolic extract of Cannabis sativa plant dissolved in normal saline per os, group C received 2.0 mg /kg body weight of Cannabis sativa extract and 40 mg/kg body weight of folic acid tablets (Emzor&#174;) and group D received 2 mg/kg body weight of folic acid per os. The rat pups from group B showed deficit in locomotor function with spongiosis and astrogliosis in the corpus callosum, cerebellum and hippocampal regions of the brain. While pups from group A showed normal locomotor activities with no visible lesions in regions of the brain such as the cerebrum, cerebellum and the hippocampus. Group C pups were presented with normal locomotor activities with congestion of vessels in cerebrum and meninges and scattered areas of cell loss in the cerebrum, cerebellum hippocampus and corpus callosum and group D pups showed normal locomotor function, with vascular congestion in the meninges and cerebrum. We, therefore, concluded that folic acid which is a crucial factor in cell division, neurotransmitter production and an antioxidant in the nervous system could have a reversal of behavioural and locomotor deficits on nervous tissues exposed to Cannabis sativa extract by reducing the rate of cell death and increasing cellular stability and integrity in brain.

影响大麻素含量的主要因素

大麻素是大麻植株中的主要活性物质,含有大麻素的产品已应用于药品、食品及化妆品等行业,而这类成分主要存在于植株的雌花、幼叶、种子苞片等部位的腺毛中。大麻素的累积不仅受自身特性的影响,还受种植环境的影响,科学地调节影响大麻素含量的主要因素,可为获得高含量大麻素的工业大麻植株提供科学的依据。

汉麻Alfin-like转录因子家族鉴定及表达分析

对汉麻Alfin-like转录因子家族进行鉴定和生物信息学分析,为Alfin-like转录因子调控汉麻生长发育及抗性奠定基础。通过利用拟南芥Alfin-like转录因子蛋白序列作为模板,利用TBtools、MEGA等生物信息学工具分析汉麻全基因组数据。在汉麻基因中共鉴定出5个CsALs基因,对其进行了理化性质分析、亚细胞定位、保守基序、基因结构、染色体定位、顺式元件、共线性分析、进化分析及聚类分析等基础分析。结果表明:鉴定的5个汉麻AL转录因子均含有DUF3594结构域(PAL结构域)和PHD手指状结构域;经理化性质分析得出,CsALs蛋白质长度在239~256 aa之间,等电点在4.97~5.31之间,亲水性为–0.809~–0.623,属于酸性亲水蛋白;CsALs基因分布在4条染色体上,亚细胞定位发现AL转录因子均定位在细胞核;上游2000 bp启动子元件分析与光响应、胁迫响应和激素相关;根据与拟南芥、大豆、水稻、玉米、烟草等系统发育分析共分为B、E、F 3个亚族;热图聚类分析表明CsALs基因在汉麻中具有较高表达。

大麻(Cannabis sativa L.)内生细菌的分离与鉴定

为分离与鉴定大麻(Cannabis sativa L.)雌株及雄株3个部位的叶片(从植株茎基向茎尖方向第二轮叶,第五轮叶,雄花周围叶)和花的内生细菌,本研究采用微生物培养和分子生物学鉴定方法,探讨其种类及其分布特征。结果显示,从大麻雄株中分离到6属35株内生细菌,大麻雌株中分离到11属39株内生细菌。内生细菌种属在大麻雌雄植株不同部位的叶和花中的分布存在显著差异,大麻雌株与雄株的内生细菌种类只共有葡萄球菌属(Staphylococcus sp.)、芽孢杆菌属(Bacillus sp.)和马赛菌属(Massilia sp.),其余均不共有。大麻雄株3个部位叶片中共有阿尔莱特葡萄球菌和溶血葡萄球菌而雄花中不存在;在雄花中分离到地衣芽孢杆菌、木糖葡萄球菌和贝莱斯芽孢杆菌而叶中不存在。大麻雌株3个部位叶片共有贝莱斯芽孢杆菌和特基拉芽孢杆菌而雌花中不存在;在雌花中分离到粪短波单胞菌、谷粒申氏菌、农杆菌、香料葡萄球菌、缺陷短波单胞菌、地衣芽胞杆菌和弗留明拜叶林克氏菌而叶中不存在。结果表明大麻内生细菌在不同植株、叶和花中的多样性以及不同内生细菌的分布差异。本研究结果为进一步研究大麻内生细菌的多样性提供科学依据,同时为未来大麻内生菌功能性促生菌种的筛选提供基础。

工业大麻转录组学研究进展

工业大麻作为多用途经济作物,其相关产业及创新性应用蓬勃发展,对其种质资源提出更高的要求,因此根据不同产业需求培育多功能、抗逆性强的品种至关重要。转录组测序技术发展迅速,已被广泛应用于农业、医学及生命科学等领域。利用转录组测序技术精准鉴定、深度发掘优异基因并解析分子机理,对工业大麻品种遗传改良及产业创新发展具有重要意义。因此,研究阐述了转录组测序技术在工业大麻的基因组组装、新基因挖掘、抗胁迫机制、分子育种及其纤维发育调控等领域的研究成果,以期为工业大麻分子育种研究提供参考。

外源H_(2)O_(2)处理对药用大麻中大麻素含量的影响

目的:通过外源活性氧调节大麻的次生代谢,提高大麻二酚(CBD)含量,为优质药用大麻生产提供新的技术。方法:在大麻始果期采摘大麻植株顶端苞片,分别采用清水和20、200、2000μmol·L^(-1)过氧化氢(H_(2)O_(2))对大麻苞片进行喷洒处理,连续4 d分析大麻体内活性氧含量、抗氧化酶活性、大麻素生物合成途径关键基因表达量及大麻素类成分含量的变化规律。结果:外源H_(2)O_(2)使大麻苞片中的H_(2)O_(2)、O_(2)^(·–)及丙二醛(MDA)含量均有所提升。20μmol·L^(-1) H_(2)O_(2)处理使大麻超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力提升最大,在处理第1~2天达到峰值。过氧化物酶(POD)在H_(2)O_(2)处理第3天达到峰值。外源H_(2)O_(2)能够明显提升聚酮合酶、四氢大麻酚酸合成酶、大麻二酚酸合成酶的基因表达量。20μmol·L^(-1) H_(2)O_(2)处理第1天大麻苞片中CBD含量比对照组提高了72.9%。结论:外源活性氧能够诱导出植物的逆境生理状态,20μmol·L^(-1) H_(2)O_(2)处理能够极显著提高大麻CBD含量。

工业大麻发酵后其CBD及CBDA含量的动态变化

旨在研究工业大麻发酵后其CBD和CBDA含量的动态变化,以阐明CBD及CBDA之间的相关性,为高效生产大麻二酚(CBD)原料提供参考。以CBD和CBDA为指标,采用酿酒酵母(Saccharomyces cerevisiae)、植物乳杆菌(Lactobacillus plantarum)、大肠杆菌(Escherichia coli)以及五味子内生细菌WF17(Bacillus subtilis)对工业大麻进行发酵,用HPLC法分析工业大麻发酵前后其CBD及CBDA含量的动态变化。酿酒酵母发酵工业大麻后CBD含量在1 d时达到最大值(3.1161 mg/g)。植物乳杆菌发酵工业大麻后CBD含量在第9天时达到最大值(3.7786 mg/g)。大肠杆菌发酵工业大麻后CBD含量在发酵3 d时达到最大值(3.5502 mg/g)。内生细菌WF17发酵工业大麻后CBD含量在发酵时间为7 d时达到最大值(3.9182 mg/g)。而CBDA含量均在发酵第1天内降低幅度最大。4种发酵菌株均能在一定程度上使发酵工业大麻中CBD的含量显著提高,CBDA含量显著下降,且在工业大麻在发酵过程中可能存在CBDA转化为CBD的过程。因此,发酵技术可以用于工业大麻中CBD的高效生产。