Rapid separation of acetophenone and its monohydroxy isomers by capillary electrophoresis

This work describes the development of a capillary electrophoresis （CE） method for the simultaneous separation of acetophenone （AP）, 2-hydroxyacetophenone （2-HAP）, 3-hydroxyacetophenone （3-HAP） and 4-hydroxyacetophenone （4-HAP） in synthetic mixtures using 10 mmol/L of sodium tetraborate buffer （pH 9.5）. The aim of this work is to demonstrate the effectiveness of CE to separate AP and its monohydroxy isomers and to define how the separations are affected by buffers, buffer pH, sample matrices and separation voltage. This method was successfully used for the trace level separation and determination of 2-HAP, 3-HAP and 4-HAP in synthetic mixture and 4-HAP in spiked plasma samples.

毛细管电泳法分析维甲酸异构体

采用毛细管电泳分析方法对维甲酸异构体进行了分离。比较了区带电泳和胶束电动色谱两种模式对维甲酸异构体的分离效果。考察了缓冲液组成、pH值、乙腈含量以及十二烷基硫酸钠(SDS)浓度等对分离的影响。采用胶束电动色谱法可完全分离13 顺 和全反式 维甲酸,最佳的缓冲溶液为(20mmol/LTris H3BO3(pH8 5) 25mmol/LSDS) 乙腈(体积比为80∶20)。该法首次应用于加标血浆中的维甲酸分析,获得了较好的结果。